SUCLG1 (NM_003849) Human Untagged Clone

CAT#: SC117713

SUCLG1 (untagged)-Human succinate-CoA ligase, alpha subunit (SUCLG1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SUCLG1
• Synonyms: GALPHA; MTDPS9; SUCLA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117713 sequence for NM_003849 edited (data generated by NextGen Sequencing)
  ATGACCGCAACCCTTGCCGCTGCCGCTGACATCGCTACCATGGTCTCCGGCAGCAGCGGC
  CTCGCCGCCGCCCGTCTCCTGTCGCGCAGCTTCCTCCTGCCGCAGAATGGAATTCGGCAT
  TGTTCCTACACAGCTTCTCGGCAACATCTCTATGTTGATAAAAATACAAAGATTATTTGC
  CAGGGTTTCACTGGCAAACAGGGCACCTTTCACAGCCAGCAGGCATTGGAATATGGCACC
  AAACTCGTTGGAGGAACCACTCCAGGGAAAGGAGGCCAGACACATCTGGGCTTACCTGTC
  TTTAATACTGTGAAGGAGGCCAAAGAACAGACAGGAGCAACGGCTTCTGTCATTTATGTT
  CCTCCGCCTTTTGCTGCTGCTGCCATTAATGAAGCTATTGAGGCAGAAATTCCCTTGGTT
  GTGTGTATCACTGAAGGAATTCCCCAGCAGGACATGGTACGAGTCAAGCACAAACTGCTG
  CGCCAGGAAAAGACAAGGCTAATTGGGCCCAACTGCCCTGGAGTCATCAATCCTGGAGAA
  TGTAAAATTGGCATCATGCCTGGCCATATTCACAAAAAAGGAAGGATTGGCATTGTGTCC
  AGATCTGGCACCCTGACTTATGAAGCAGTTCACCAAACAACGCAAGTTGGATTGGGGCAG
  TCTTTGTGCGTTGGCATTGGAGGTGATCCTTTTAATGGAACAGATTTTATTGACTGCCTC
  GAAATCTTTTTGAACGATTCTGCCACAGAAGGCATCATATTGATTGGTGAAATTGGTGGT
  AATGCAGAAGAGAATGCTGCAGAATTTTTGAAGCAACATAATTCAGGTCCAAATTCCAAG
  CCTGTAGTGTCCTTCATTGCTGGTTTAACTGCTCCTCCTGGGAGAAGAATGGGTCATGCC
  GGGGCAATTATTGCTGGAGGAAAAGGTGGAGCTAAAGAGAAGATCTCTGCCCTTCAGAGT
  GCAGGAGTTGTGGTCAGTATGTCTCCTGCACAGCTGGGAACCACGATCTACAAGGAATTT
  GAAAAGAGGAAGATGCTATGA
  
  Clone variation with respect to NM_003849.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003849 unedited
  TTTTGTATACGACTCCTATAGGCGGCCGCGAATTCGGCACGAGGGAGAGAGAGAGAGAGA
  GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
  GAGAGATGACCGCAACCCTTGCCGCTGCCGCTGACATCGCTACCATGGTCTCCGGCAGCA
  GCGGCCTCGCCGCCGCCCGTCTCCTGTCGCGCAGCTTCCTCCTGCCGCAGAATGGAATTC
  GGCATTGTTCCTACACAGCTTCTCGGCAACATCTCTATGTTGATAAAAATACAAAGATTA
  TTTGCCAGGGTTTCACTGGCAAACAGGGCACCTTTCACAGCCAGCAGGCATTGGAATATG
  GCACCAAACTCGTTGGAGGAACCACTCCAGGGAAAGGAGGCCAGACACATCTGGGCTTAC
  CTGTCTTTAATACTGTGAAGGAGGCCAAAGAACAGACAGGAGCAACGGCTTCTGTCATTT
  ATGTTCCTCCGCCTTTTGCTGCTGCTGCCATTAATGAAGCTATTGAGGCAGAAATTCCCT
  TGGTTGTGTGTATCACTGAAGGAATTCCCCAGCAGGACATGGTACGAGTCAAGCACAAAC
  TGCTGCGCCAGGAAAAGACAAGGCTAATTGGGCCCAACTGCCCTGGAGTCATCAATCCTG
  GAGAATGTAAAATTGGCATCATGCCTGGCCATATTCACAAAANAGGAAGGATTGGCATTG
  TGTCCAGATCTGGCACCCTGACTTATGAAGCAGNTCACCANNACAACGCAGTTGGATTGG
  GGCAGTCTTTGTGCGTTGGCATTGGNAGTGATCCTTTNTATGGAAACAGATTTATTGACT
  GCCTCCAAATCTTTTTGACGATNCTGCCACAGAAGCATCATATTGATTGTGAAAATGGTG
  GC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003849 unedited
  GACCGCGGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGAAATAAATAGTAGA
  TTTATTGGCTGTCTCTGTAATACAATGTGGTGAAAACGTCTTAATTCAGGACATCTTCCA
  CCTTGTTTTGGCTTCCAGTTGTACTGCAAGACCAGTGTCAGGCACATAGGCTGATTAATC
  AGTGGACAACAGAAGCAAACTGCTGCTGGGTTACATGTCTACGTGATCCATTCCACAGTT
  TTAGGAATTTTTTTTTTCTTTCATAGCATCTTCCTCTTTTCAAATTCCTTGTAGATCGTG
  GTTCCCAGCTGTGCAGGAGACATACTGACCACAACTCCTGCACTCTGAAGGGCAGAGATC
  TTCTCTTTAGCTCCACCTTTTCCTCCAGCAATAATTGCCCCGGCATGACCCATTCTTCTC
  CCAGGAGGAGCAGTTAAACCAGCAATGAAGGACACTACAGGCTTGGAATTTGGACCTGAA
  TTATGTTGCTTCAAAAATTCTGCAGCATTCTCTTCTGCATTACCACCAATTTCACCAATC
  AATATGATGCCTTCTGTGGCAGAATCGTTCAAAAAGATTTCGAGGCAGTCAATAAAATCT
  GTCCCATTAAAAGATCACCTTCCATGCCAACGCACAAAGACTGCCCCAATCCAACTTGCG
  TTGTTTGGTGAACTGCTTCATAAGTCAAGGTGCCAGATCTGGACACAATGCCAATCCTTC
  CTTTTTTGTGAAAATGGCCAGCCATGATGCCCATTTTTACATTTTCCCAGATTGATGACT
  CCAGGGCAGTTTGGGCCCAATAACCTTGTCTTTTCCTGCCCCACAAGTTGGGCTTGATTC
  TACCATGTCCTGCTGGGGAATTCTTTAGGATACCCCAAACCAAGAAATTCTGCCTCAATA
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003849
• Insert Size: 1390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003849.1, NP_003840.1
• RefSeq Size: 1227 bp
• RefSeq ORF: 1002 bp
• Locus ID: 8802
• UniProt ID: P53597
• Cytogenetics: 2p11.2
• Domains: CoA_binding, ligase-CoA
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Propanoate metabolism
• Gene Summary: This gene encodes the alpha subunit of the heterodimeric enzyme succinate coenzyme A ligase. This enzyme is targeted to the mitochondria and catalyzes the conversion of succinyl CoA and ADP or GDP to succinate and ATP or GTP. Mutations in this gene are the cause of the metabolic disorder fatal infantile lactic acidosis and mitochondrial DNA depletion. [provided by RefSeq, Feb 2010]