GGT5 (NM_004121) Human Untagged Clone

CAT#: SC117572

GGT5 (untagged)-Human gamma-glutamyltransferase 5 (GGT5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GGT5
• Synonyms: GGL; GGT-REL; GGT 5; GGTLA1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117572 sequence for NM_004121 edited (data generated by NextGen Sequencing)
  ATGGCCCGGGGCTACGGGGCCACGGTCAGCCTAGTCCTGCTGGGTCTGGGGCTGGCGCTG
  GCTGTCATTGTGCTGGCTGTGGTCCTCTCTCGACACCAGGCCCCATGTGGCCCCCAGGCC
  TTTGCCCACGCTGCTGTTGCCGCCGACTCCAAGGTCTGCTCGGATATTGGACGAGCCATC
  CTCCAGCAGCAGGGCTCACCCGTGGATGCCACCATCGCGGCTCTGGTCTGCACCAGCGTC
  GTCAACCCTCAGAGCATGGGCCTGGGCGGAGGGGTCATCTTCACCATCTACAATGTGACA
  ACAGGGAAGGTGGAGGTCATCAATGCCCGGGAGACGGTGCCGGCCAGCCACGCCCCGAGC
  CTGCTGGACCAGTGTGCACAGGCTCTGCCACTGGGCACAGGGGCCCAGTGGATCGGGGTG
  CCCGGGGAGCTCCGTGGCTATGCCGAGGCCCACCGCCGCCATGGCCGCCTGCCCTGGGCG
  CAGCTGTTCCAGCCCACCATCGCGCTGCTCCGAGGGGGGCATGTGGTGGCCCCTGTCCTC
  AGCCGTTTCCTGCACAACAGCATCCTGCGGCCTTCCTTGCAGGCGTCAACCCTGCGCCAG
  CTCTTCTTCAACGGGACAGAACCCCTGAGGCCTCAGGACCCACTCCCATGGCCTGCACTG
  GCCACCACCCTGGAGACCGTGGCCACAGAGGGCGTGGAGGTCTTCTACACGGGGAGGCTG
  GGCCAGATGCTGGTGGAGGACATTGCCAAGGAAGGGAGCCAGCTGACGCTGCAGGACCTG
  GCCAAGTTCCAGCCCGAGGTGGTGGATGCCCTGGAGGTGCCCCTGGGGGACTATACCCTG
  TACTCACCACCGCCGCCTGCAGGGGGTGCCATTCTCAGCTTTATCCTCAACGTGCTAAGA
  GGGTTCAACTTCTCAACAGAGTCTATGGCCAGGCCTGAAGGGAGGGTGAACGTGTACCAC
  CACCTTGTAGAGACGCTCAAGTTTGCCAAGGGGCAGAGGTGGAGGCTGGGGGACCCTCGA
  AGCCACCCGAAGCTCCAGAATGCCTCCCGGGACCTGCTGGGGGAGACCCTGGCCCAGCTC
  ATCCGCCAACAGATCGATGGCCGGGGGGACCACCAGCTCAGCCACTACAGCTTGGCCGAG
  GCCTGGGGCCACGGGACAGGCACGTCCCATGTGTCTGTGCTGGGGGAGGATGGCAGCGCC
  GTGGCTGCCACCAGCACCATCAACACACCCTTTGGAGCGATGGTGTATTCACCACGGACA
  GGCATCATCCTCAACAACGAGCTCCTGGACTTATGCGAGCGATGCCCCCGGGGTTCCGGC
  ACCACCCCCTCACCTGTGAGTGGAGACAGGGTGGGTGGAGCTCCCGGAAGGTGCTGGCCC
  CCAGTTCCAGGCGAGCGTTCCCCATCCTCCATGGTGCCCTCCATCTTGATCAACAAAGCC
  CAGGGGTCGAAGCTAGTGATTGGCGGGGCTGGCGGGGAGCTCATCATCTCTGCTGTGGCC
  CAGGCCATCATGAGCAAGCTGTGGCTTGGCTTTGACCTGAGAGCGGCCATTGCAGCCCCC
  ATCCTGCATGTCAACAGCAAGGGCTGTGTGGAGTACGAGCCCAACTTCAGCCAGGAGGTG
  CAGAGGGGACTCCAAGACCGTGGCCAGAACCAGACCCAGAGGCCCTTCTTCCTGAACGTG
  GTCCAGGCTGTGTCCCAGGAGGGGGCCTGTGTGTACGCCGTCTCGGACCTGAGGAAGAGT
  GGGGAGGCCGCAGGCTACTAA
  
  Clone variation with respect to NM_004121.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004121 unedited
  GTTCTTTCCCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGGGGAACCCGGGGAATGGTGTGGAGGTTCCAGGGAGCCCTGCCC
  CTACCTGGCAACCGCAGTGCAGCAGGCACCAAGTTCTCCTGCACATTGCGACAGTGTGAC
  CCTGGGCTCTGGCGGGCGGTAGGTGGGGCCTTTGGACCTACTAGCAGTGAGGGAGTTAAC
  ACAGCAGCTGACTCCTCTAGGCAAGGAAAACTCCCCTCAGACGCTTTGCTGCCTGGCCTC
  CTGCCAGCAACAAGCAGGAGCTGAAAACCAGAAGTTGAGGCGTGAGTTTGGCCACTCCGT
  AGTGTGCACTTGGTGAGGGCAGCAGCTCGCCACAGCTGCCAGCCATCTGTCCATTCACCC
  ATCTGTCCATCTGGCAGCCCGCTGTTCAGACCTGTCTGTCTGTCCGCCCATCTGTAAGCC
  CATCTCTGTCCCATTGTCTATCTGACCATCTTTCTCTTACTGTCCTCTTTGTCTAGCTAT
  CTGGCCTATCTGTCGATCCATCTTCGTGTCTGTCTTCAGCCCCCACCTGTTTGTCCATCT
  GTCCAATTACCTGTGACTCTGTGCATCTTCTTGTCCATTCATCTGCCCACCCATCCGTCC
  CTCCGTCTGCCACCAGCCGNCCCTCTCCTCCTGGGCTGCAGAGCCATGGCCCGGGGCTAC
  GGNGCCACGGTCAGCCTATCCTGCNTGGTCTGNNNGCTGCGCCTGCTGTCATTGTGCTGC
  CTGTGGTCCTCTCTCGACACAGCCCCCATGTGGCCCCCAGNCCTTGCCACGCTGCTGTGC
  CGCCGATCCAAGTCTGCTCGATTTTGGACGAGCCTCCTCCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004121 unedited
  NTGGGTTTACTATGNACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGTT
  TTTTTTTTTTTTTTTTTTTCACAGTCCGCCTCTTTAATCTTGGACTGGAGGATATACAGA
  TCGAGAGGGTGGGAGAGTGGCCCCAGGCAAGAGGCCTGCGGAGGGATAGAGGGGCCGGTT
  CAGGACCACCAGGGGCTCTGGGAAAGGGGGTTAGGGATGAGGCTCAACCTCTCATCTGCC
  CAGCTGGGCCCCGCCACCTTTTCCACTCTCAGCTGGACTCCCCTGCCAGGGGTCCAGATC
  CTGCCAGAGTAGTTGGTCCCCCAGCCATGTCCGGCCTGGACACAGGACTCATGGGGGGGC
  CAGACTTTAGCTCTGGGCAAAGCAGTGTCTTAATAGCCTGGGGCCTCCCCACTCTTCCTC
  AGGTCCGAGACGGGGTACACACAGGCCCCCTCCTGGGACACAGCCTGGACCACGTTCAGG
  AAGAAAGGCCTCTGGGTCTGGTTCTGGCCACGGTCTTGGAGTCCCCTCTGCACCTCCTGT
  AATGCCAGCAATTTGGGAGGCTGAGGAAGGAGGATCACTTGAGGCCAGGAGTTTGAGACC
  AACCTGGGCAATGCAGCAAGATCCTGTTTCTGGCTGAAGTTGGGCTCGTACTCCACAAAA
  CCCTTGCTGTTTACTGCAAGATGGGGGCTGGAATGGGCGCTCTTAGGGCAAAGACAAACC
  CCAGTTTGCTTATGATGGCCCTGGGCCCACCAAAAAAAATAAGCTCCCCCCCGCCCCGCC
  AATCAATATCTTTTACCCCGGGGTTTTTTTTAACAATGGAGGGCACCCTGGAGGATGGGG
  AAACCTTCCCTGGAAATGGGGGGCCCCCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004121
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004121.1, NP_004112.1
• RefSeq Size: 2414 bp
• RefSeq ORF: 1761 bp
• Locus ID: 2687
• UniProt ID: P36269
• Cytogenetics: 22q11.23
• Domains: G_glu_transpept
• Protein Families: Protease, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Cyanoamino acid metabolism, Glutathione metabolism, Metabolic pathways, Selenoamino acid metabolism, Taurine and hypotaurine metabolism
• Gene Summary: This gene is a member of the gamma-glutamyl transpeptidase gene family, and some reports indicate that it is capable of cleaving the gamma-glutamyl moiety of glutathione. The protein encoded by this gene is synthesized as a single, catalytically-inactive polypeptide, that is processed post-transcriptionally to form a heavy and light subunit, with the catalytic activity contained within the small subunit. The encoded enzyme is able to convert leukotriene C4 to leukotriene D4, but appears to have distinct substrate specificity compared to gamma-glutamyl transpeptidase. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is one amino acid shorter than isoform 1.