Dishevelled 3 (DVL3) (NM_004423) Human Untagged Clone

CAT#: SC117370

DVL3 (untagged)-Human dishevelled, dsh homolog 3 (Drosophila) (DVL3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Dishevelled 3
• Synonyms: DRS3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004423 edited
  GAATTCGGCACGAGGCCGGTAGCGGCCGGAGAACAAGGGAGCTGGCGCCGCCAGCAGCCG
  CCGAGCTGGGTTGAGCCGCTGGGCCGCGCCGCGCGCCGCCGCCGTCTGGGAGGCTCGGCC
  CGGCCGCCCGAGCAGGCCGCGCGCGGGCCGCCGGGCCCGAGGCCAGAGCCATGGGCGAGA
  CCAAGATCATCTACCACTTGGATGGGCAGGAGACGCCGTACCTTGTGAAGCTGCCCCTGC
  CCGCCGAGCGCGTCACCTTGGCGGACTTTAAGGGCGTTTTGCAGCGACCCAGCTATAAGT
  TCTTCTTCAAGTCTATGGACGACGATTTCGGAGTGGTGAAGGAGGAGATCTCGGATGACA
  ATGCCAAGCTACCATGCTTCAATGGCCGGGTGGTGTCCTGGCTGGTGTCAGCTGAGGGCT
  CACACCCAGACCCAGCCCCCTTCTGTGCTGATAACCCATCGGAGCTGCCACCACCTATGG
  AGCGCACGGGAGGCATCGGGGACTCCCGACCCCCATCCTTCCACCCTCATGCTGGTGGGG
  GCAGCCAGGAGAACCTGGACAATGACACAGAGACGGACTCTTTGGTGTCTGCCCAGCGAG
  AGCGGCCACGCCGGAGGGATGGCCCAGAGCATGCAACCCGGCTAAATGGAACTGCGAAGG
  GGGAACGGCGGCGAGAACCAGGGGGTTATGATAGCTCATCCACCCTTATGAGCAGTGAGC
  TGGAGACCACCAGCTTCTTTGACTCAGATGAGGATGACTCCACCAGCAGGTTCAGCAGCT
  CCACAGAACAGAGCAGTGCCTCACGCCTGATGAGAAGACACAAGCGGCGGCGGCGGAAGC
  AGAAGGTTTCTCGGATTGAGCGGTCCTCGTCCTTCAGCAGCATCACGGACTCCACCATGT
  CACTCAACATCATCACGGTCACTCTCAACATGGAAAAATATAACTTCTTGGGCATCTCCA
  TTGTGGGCCAAAGCAACGAGCGTGGTGACGGCGGCATCTACATTGGCTCTATCATGAAGG
  GTGGGGCCGTGGCTGCTGATGGACGCATCGAGCCAGGAGATATGTTGTTACAGGTAAACG
  AGATCAACTTTGAGAACATGAGTAATGACGATGCAGTCCGGGTACTGCGGGAGATTGTGC
  ACAAACCGGGGCCCATCACCCTGACTGTAGCCAAGTGCTGGGACCCAAGTCCACGTGGTT
  GCTTCACATTGCCCAGGAGCGAGCCCATCCGGCCCATTGACCCTGCGGCCTGGGTCTCCC
  ACACTGCAGCCATGACCGGCACCTTCCCTGCATACGGCATGAGCCCCTCCCTGAGCACCA
  TCACCTCCACCAGCTCCTCCATCACCAGTTCCATCCCTGACACAGAGCGCCTAGACGACT
  TCCACTTGTCCATCCACAGTGACATGGCTGCCATCGTAAAAGCCATGGCCTCCCCTGAAT
  CAGGGTTGGAGGTCCGTGACCGCATGTGGCTCAAGATTACCATCCCTAATGCTTTCATCG
  GCTCAGATGTGGTGGACTGGCTGTACCACAATGTGGAAGGCTTCACGGACCGGAGGGAGG
  CCCGCAAGTATGCCAGCAACCTGCTGAAAGCTGGCTTCATCCGCCATACCGTCAACAAGA
  TCACCTTCTCCGAGCAGTGCTACTACATCTTCGGTGACCTCTGCGGCAACATGGCCAACC
  TGTCTCTCCACGATCACGATGGCTCCAGTGGCGCCTCTGACCAGGACACACTGGCCCCTT
  TGCCGCACCCGGGGGCCGCCCCTTGGCCCATGGCTTTCCCGTACCAGTACCCGCCACCCC
  CGCACCCATACAACCCGCACCCGGGCTTCCCGGAGCTGGGCTACAGCTACGGCGGGGGCA
  GCGCCAGCAGTCAGCACAGCGAAGGCAGTCGGAGCAGTGGCTCCAACCGTAGCGGCAGCG
  ATCGGAGGAAGGAGAAGGACCCGAAGGCCGGGGACTCCAAGTCCGGGGGCAGCGGCAGCG
  AATCGGACCACACCACACGCAGCAGCCTGCGGGGGCCGCGGGAGCGGGCGCCCAGCGAGC
  GCTCAGGGCCGGCGGCCAGCGAGCACAGCCACCGCAGCCACCATTCCCTGGCCAGCAGCC
  TTCGCAGCCACCACACACACCCGAGCTACGGTCCTCCCGGAGTGCCCCCTCTCTACGGCC
  CCCCCATGCTGATGATGCCCCCGCCGCCCGCGGCCATGGGGCCCCCAGGAGCCCCTCCGG
  GCCGCGACCTGGCCTCAGTGCCCCCGGAACTGACCGCCAGCAGACAGTCCTTCCGCATGG
  CCATGGGAAACCCCAGTGAGTTCTTTGTGGATGTGATGTGAGCAGGGCCCCTCCCCCAGC
  TCCATTCCGCTCCCACCCCAGCCGGCTGCGTTCCTCTCTCCATCCGTCCGTCTTTTTTAC
  TTTGTCTGGTACCTGAAAGGGAAATAAAAGGAACTAAATCCAGGTGCGCTAACTGCTCGC
  AGGGTGCTGCGAGGGTGGGGTGCACCTACCGATTGGCTCTGCAGCCCCCTAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004423 unedited
  NGGTGCATATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCCGGTAGC
  GGCCGGAGAACAAGGAGCTGGCGCCGCCAGCAGCCGCCGAGCTGGGTTGAGCCGCTGGGC
  CGCGCCGCGCGCCGCCGCCGTCTGGGAGGCTCGGCCCGGCCGCCCGAGCAGGCCGCGCGC
  GGGCCGCCGGGCCCGAGGCCAGAGCCATGGGCGAGACCAAGATCATCTACCACTTGGATG
  GGCAGGAGACGCCGTACCTTGTGAAGCTGCCCCTGCCCGCCGAGCGCGTCACCTTGGCGG
  ACTTTAAGGGCGTTTTGCAGCGACCCAGCTATAAGTTCTTCTTCAAGTCTATGGACGACG
  ATTTCGGAGTGGTGAAGGAGGAGATCTCGGATGACAATGCCAAGCTACCATGCTTCAATG
  GCCGGGTGGTGTCCTGGCTGGTGTCAGCTGAGGGCTCACACCCAGACCCAGCCCCCTTCT
  GTGCTGATAACCCATCGGAGCTGCCACCACCTATGGAGCGCACGGGAGGCATCGGGGACT
  CCCGACCCCCATCCTTCCACCCTCATGCTGGTGGGGGCAGCCAGGAGAACCTGGACAATG
  ACACAGAGACGGACTCTTTGGTGTCTGCCCAGCGAGAGCGGCCACGCCGGAGGGATGGCC
  CAGAGCATGCAACCCGGCTAAATGGAACTGCGAAGGGGGAACGGCGGCGAGAACCGNGGG
  TTATGATAGCTCATCCACCCTTATGAGCAGTGAGCTGGAGACCACCAGCTTCTTTGACTC
  AGATGAGGATGACTCCACCAGCAGGTTCAGCAGCTTCACAACAAAAAGCAGTGCCTCACG
  CCTGATGAGAAGACACAAGCGGCCGCGGCGGAAGCAGAAGGTTCTCGGATTGAGCGGTTC
  TCGTCCTCAGCAGCATCACGACTCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004423 unedited
  GGGGGACCGGGGCTCAAGCCCCCCACATTAAAAANNNNGNNNGTACGTGANACGAGAGCC
  GCATCNNANATNCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTAGGGGGCTGGAAAACCAATCGGGAGGGGCACCCCACCCTTGCAACACC
  CTGGGAGCAGTTAGCGCACCTGGATTTAATTCCTTTTATTTCCCTTTCAGGGACCAAACA
  AAGTAAAAAAAACGGACGGTTTGTAAAAAAGAACCCACCCGGCTGGGGGGGGAACGGAAT
  GGAACTGGGGGAGGGGCCCTGTTTACATTACATCCACAAAAAACTCACTGGGGTTTCCCA
  TGGCCATGCGGAAGGACTGTTTGCTGGCGGTCAATTCCGGGGGCACTGAGGCCAAGTCGC
  GGCCCGGAGGGGCTCCTGGGGGCCCCATGGCCGGGGGCGGGGGGGGCATTATCAACATGG
  GGGGGCCGTAAAAAGGGGGCACTCCGGGAGGACCGTAACTCGGGTGTGTGTGGGGGCTGC
  AAAAGCTGCTGGCCAAGGAATGGGGGCTGCGGGGGCTGTGCTCCCTGGCCCCCGGCCCTG
  AACGCTTGCTGGGCGCCCGCTCCCGCGGCCCCCCAAAGCTGTTGGGTGGGGGGTGGGCCA
  ATTCGCTGCCGGTGGCCCCGGACTTGGGAATCCCGGCCTTTGGGTCCTTTTCCTTTCTTC
  CATCGCTGCCGCTACGGGTTGGAACCAATGGTTCCAATGGCTTCTCTTGGGTGACTGGTG
  GGCCTGCCCCCCCCGTAACTGTAACCCACCCTCCGGAAACCCCGGTGCCGGTTTTAATGG
  GTGCGGGGGGGGGGGGTACTCTGTCCGGAAAACCCAGTGGCAAGGGGCGCCCCCCGGGGG
  CCCAAAAGGGCCATGGGTTCTTGTAAAAGCGCCCTTGGAACCATGGAACCGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004423
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004423.3, NP_004414.3
• RefSeq Size: 5062 bp
• RefSeq ORF: 2151 bp
• Locus ID: 1857
• UniProt ID: Q92997
• Cytogenetics: 3q27.1
• Domains: DEP, DAX, PDZ, Dishevelled
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• Gene Summary: This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq, Jul 2008]