WNT7A (NM_004625) Human Untagged Clone

CAT#: SC117232

WNT7A (untagged)-Human wingless-type MMTV integration site family, member 7A (WNT7A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WNT7A
• Synonyms: Wnt-7a
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004625 edited
  GAATTCGGCACCAGCGCGGACGGGCGCACCGACAGACGGCCCCGGGGACGCCTCGGCTCG
  CGCCTCCCGGGCGGGCTATGTTGATTGCCCCGCCGGGGCCGGCCCGCGGGATCAGCACAG
  CCCGGCCCGCGGCCCCGGCGGCCAATCGGGACTATGAACCGGAAAGCGCGGCGCTGCCTG
  GGCCACCTCTTTCTCAGCCTGGGCATGGTCTACCTCCGGATCGGTGGCTTCTCCTCAGTG
  GTAGCTCTGGGCGCAAGCATCATCTGTAACAAGATCCCAGGCCTGGCTCCCAGACAGCGG
  GCGATCTGCCAGAGCCGGCCCGACGCCATCATCGTCATAGGAGAAGGCTCACAAATGGGC
  CTGGACGAGTGTCAGTTTCAGTTCCGCAATGGCCGCTGGAACTGCTCTGCACTGGGAGAG
  CGCACCGTCTTCGGGAAGGAGCTCAAAGTGGGGAGCCGGGAGGCTGCGTTCACCTACGCC
  ATCATTGCCGCCGGCGTGGCCCACGCCATCACAGCTGCCTGTACCCAGGGCAACCTGAGC
  GACTGTGGCTGCGACAAAGAGAAGCAAGGCCAGTACCACCGGGACGAGGGCTGGAAGTGG
  GGTGGCTGCTCTGCCGACATCCGCTACGGCATCGGCTTCGCCAAGGTCTTTGTGGATGCC
  CGGGAGATCAAGCAGAATGCCCGGACTCTCATGAACTTGCACAACAACGAGGCAGGCCGA
  AAGATCCTGGAGGAGAACATGAAGCTGGAATGTAAGTGCCACGGCGTGTCAGGCTCGTGC
  ACCACCAAGACGTGCTGGACCACACTGCCACAGTTTCGGGAGCTGGGCTACGTGCTCAAG
  GACAAGTACAACGAGGCCGTTCACGTGGAGCCTGTGCGTGCCAGCCGCAACAAGCGGCCC
  ACCTTCCTGAAGATCAAGAAGCCACTGTCGTACCGCAAGCCCATGGACACGGACCTGGTG
  TACATCGAGAAGTCGCCCAACTACTGCGAGGAGGACCCGGTGACCGGCAGTGTGGGCACC
  CAGGGCCGCGCCTGCAACAAGACGGCTCCCCAGGCCAGCGGCTGTGACCTCATGTGCTGT
  GGGCGTGGCTACAACACCCACCAGTACGCCCGCGTGTGGCAGTGCAACTGTAAGTTCCAC
  TGGTGCTGCTATGTCAAGTGCAACACGTGCAGCGAGCGCACGGAGATGTACACGTGCAAG
  TGAGCCCCGTGTGCACACCACCCTCCCGCTGCAAGTCAGATTGCTGGGAGGACTGGACCG
  TTTCCAAGCTGCGGGCTCCCTGGCAGGATGCTGAGCTTGTCTTTTCTGCTGAGGAGGGTA
  CTTTTCCTGGGTTTCCTGCAGGCATCCGTGGGGGAAAAAAAATCTCTCAGAGCCCTCAAC
  TATTCTGTTCCACACCCAATGCTGCTCCACCCTCCCCCAGACACAGCCCAGGTCCCTCCG
  CGGCTGGAGCGAAGCCTTCTGCAGCAGGAACTCTGGACCCCTGGGCCTCATCACAGCAAT
  ATTTAACAATTTATTCTGATAAAAATAATATTAATTTATTTAATTAAAAAGAATTCTTCC
  ACCTCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004625 unedited
  GGCGCGAATTTGAAACACGATTTACTATAGGGCGGCCGCGCAATTCGCACCAGCGCGGAC
  GGTCGCACCGACAGACGGCCCCGGGGACGCCTCGGCTCGCGCCTCCCGGGCGGGCTATGT
  TGATTGCCCCGCCGGGGCCGGCCCGCGGGATCAGCACAGCCCGGCCCGCGGCCCCGGCGG
  CCAATCGGGACTATGAACCGGAAAGCGCGGCGCTGTCTGGGCCACCTCTTTCTCAGCCTG
  GGCATGGTCTACCTCCGGATCGGTGGCTTCTCCTCAGTGGTAGCTCTGGGCGCAAGCATC
  ATCTGTAACAAGATCCCAGGCCTGGCTCCCAGACAGCGGGCGATCTGCCAGAGCCGGCCC
  GACGCCATCATCGTCATAGGAGAAGGCTCACAAATGGGCCTGGACGAGTGTCAGTTTCAG
  TTCCGCAATGGCCGCTGGAACTGCTCTGCACTGGGAGAGCGCACCGTCTTCGGGAAGGAG
  CTCAAAGTGGGGAGCCGGGAGGCTGCGTTCACCTACGCCATCATTGCCGCCGGCGTGGCC
  CACGCCATCACAGCTGCCTGTACCCAGGGCAACCTGAGCGACTGTGGCTGCGACAAAGAG
  AAGCAAGGCCAGTACCACCGGGACGAGGGCTGGAAGTGGGGTGGCTGCTCTGCCGACATC
  CGCTACGGCATCGGCTTCGCCAAGGTCTTTGTGGATGCCCGGGAGATCAAGCAGAATGCC
  CGGACTCTCATGAACTTGCACAACAACGATGCAGGCCGAAAGATCCTGGAGGAGAACATG
  AAGCTGCAATGTAGTGCCACGGTGTGTCAGGCTCCTGCACCCCCAGACGTGCTGTACCAC
  CTGCCACAGTTCCTGCAGCTGGGCTACGTGCCCAAGACAAGTACAACGAGCCGCTCACTT
  GGACCTGTGCGGCCACCCGCAACAACGGCCAACCTTCTGTAGACCAGAAGCACTGCCGTA
  CCGCAGTCCATGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004625 unedited
  TCCATTAGGGCATTGGCTGATGGCAACTTCCCAGGNCACAGGGAAAGGCACTGGAGGCAG
  GGGCTCACAGGGCATGCCACCCGGGTATCTGTTCAGAACAGCTATGACCGCGGCCGCAAT
  CTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGGTGGAAGAATTCTTTTTAATTAAATAAA
  TTAATATTATTTTTATCAGAATAAATTGTTAAATATTGCTGAGAAGAGGCCCAGGGGTCC
  AGAGTTCCTGCTGCAGAAGGCTTCGCTCCAGCCGCGGAGGGACCTGGGCTGTGTCTGGGG
  GAGGGTGGAGCACCATTGGGTGTGGAACAGAATAGTTGAGGGCTCTGAGAGATTTTTTTT
  CCCCCACGGATGCCTGCAGGAAACCCAGGAAAAGTACCCTCCTCAACAAAAAAGACAAGC
  TCAGCATCCTGCCAGGGAGCCCGCAGCTTGGAAACGGTCCAGTCCTCCCAGCAATCTGAC
  TTGCAGCGGGAGGGTGGTGTGCACACGGGGCTCACTTGCACGTGTACATCTCCGTGCGCT
  CGCTGCACGTGTTGCACTTGACATAGCACCACCAGTGGAACTTACAGTTGCACTGCCACA
  CACGGGCGTACTGGTGGGTGTTGTAGCCACGCCCACAGCACATGAGGTCACAGCCGCTGG
  CCTGGGGAGCCGTCTTGTTGCAGGCGCGGCCCTGGGTGCCCACACTGCCCGTCACCGGGT
  CCTCCTCGCAATATTTGGGCGACTTCTCGATGTACACCAAGTCCGTGTCCATGGGCTTGC
  GGTACGACAGTGGCTTCTTGGATCTTAAAGAAAGCGGGCCCCCTGTTGCCGCCCGGCCCG
  CCCCAGGCTTCACGTGAAAGGCCCCCGTTGCACTTGTCCCTGAACCACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004625
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004625.3, NP_004616.2
• RefSeq Size: 1732 bp
• RefSeq ORF: 1050 bp
• Locus ID: 7476
• UniProt ID: O00755
• Cytogenetics: 3p25.1
• Domains: wnt
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. [provided by RefSeq, Jul 2008]