CLOCK (NM_004898) Human Untagged Clone

CAT#: SC117088

CLOCK (untagged)-Human clock homolog (mouse) (CLOCK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLOCK
• Synonyms: bHLHe8; KAT13D
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117088 sequence for NM_004898 edited (data generated by NextGen Sequencing)
  ATGTTGTTTACCGTAAGCTGTAGTAAAATGAGCTCGATTGTTGACAGAGATGACAGTAGT
  ATTTTTGATGGGTTGGTGGAAGAAGATGACAAGGACAAAGCGAAAAGAGTATCTAGAAAC
  AAATCTGAAAAGAAACGTAGAGATCAATTTAATGTTCTCATTAAAGAACTGGGATCCATG
  CTTCCTGGTAATGCTAGAAAGATGGACAAATCTACTGTTCTGCAGAAAAGCATTGATTTT
  TTACGAAAACATAAAGAAATCACTGCACAGTCAGATGCTAGTGAAATTCGACAGGACTGG
  AAACCTACATTCCTTAGTAATGAAGAGTTTACACAATTAATGTTAGAGGCTCTTGATGGT
  TTTTTTTTAGCAATCATGACAGATGGAAGCATAATATATGTGTCTGAGAGTGTAACTTCA
  TTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTTATCCCAGAA
  GGGGAACATTCAGAGGTTTATAAAATACTCTCTACTCATCTGCTGGAAAGTGATTCATTA
  ACCCCAGAATATTTAAAATCAAAAAATCAGTTAGAATTCTGTTGTCACATGCTGCGAGGA
  ACAATAGACCCAAAGGAGCCATCTACCTATGAATATGTAAAATTTATAGGAAATTTCAAA
  TCTTTAAACAGTGTATCCTCTTCAGCACACAATGGTTTTGAAGGAACTATACAACGCACA
  CATAGGCCATCTTATGAAGATAGAGTTTGTTTTGTAGCTACTGTCAGGTTAGCTACACCT
  CAGTTCATCAAGGAAATGTGCACTGTTGAAGAACCCAATGAAGAGTTTACATCTAGACAT
  AGTTTAGAATGGAAGTTTCTGTTTCTAGATCACAGGGCACCACCCATAATAGGGTATTTG
  CCATTTGAAGTTCTGGGAACATCAGGCTATGATTACTATCATGTGGATGACCTAGAAAAT
  TTGGCAAAATGTCATGAGCACTTAATGCAATATGGGAAAGGCAAATCATGTTATTATAGG
  TTCCTGACTAAGGGGCAACAGTGGATTTGGCTTCAGACTCATTATTATATCACTTACCAT
  CAGTGGAATTCAAGGCCAGAGTTTATTGTTTGTACTCACACTGTAGTAAGTTATGCAGAA
  GTTAGGGCTGAAAGACGACGAGAACTTGGCATTGAAGAGTCTCTTCCTGAGACAGCTGCT
  GACAAAAGCCAAGATTCTGGGTCAGATAATCGTATAAACACAGTCAGTCTCAAGGAAGCA
  TTGGAAAGGTTTGATCACAGCCCAACCCCTTCTGCCTCTTCTCGGAGTTCAAGAAAATCA
  TCTCACACGGCCGTCTCAGACCCTTCCTCAACACCAACCAAGATCCCGACGGATACGAGC
  ACTCCACCCAGGCAGCATTTACCAGCTCATGAGAAGATGGTGCAAAGAAGGTCATCATTT
  AGTAGTCAGTCCATAAATTCCCAGTCTGTTGGTTCATCATTAACACAGCCAGTGATGTCT
  CAAGCTACAAATTTACCAATTCCACAAGGCATGTCCCAGTTTCAGTTTTCAGCTCAATTA
  GGAGCCATGCAACATCTGAAAGACCAATTGGAACAACGGACACGCATGATAGAAGCAAAT
  ATTCATCGGCAACAAGAAGAACTAAGAAAAATTCAAGAACAACTTCAGATGGTCCATGGT
  CAGGGGCTGCAGATGTTTTTGCAACAATCAAATCCTGGGTTGAATTTTGGTTCCGTTCAA
  CTTTCTTCTGGAAATTCATCTAACATCCAGCAACTTGCACCTATAAATATGCAAGGCCAA
  GTTGTTCCTACTAACCAGATTCAAAGTGGAATGAATACTGGACACATTGGCACAACTCAG
  CACATGATACAACAACAGACTTTACAGAGTACATCAACTCAGAGTCAACAAAATGTACTG
  AGTGGGCACAGTCAGCAAACATCTCTACCCAGTCAGACACAGAGCACTCTTACAGCCCCA
  CTGTATAACACTATGGTGATTTCTCAGCCTGCAGCCGGAAGCATGGTCCAGATTCCATCT
  AGTATGCCACAAAACAGCACCCAGAGTGCTGCAGTAACTACATTCACTCAGGACAGGCAG
  ATAAGATTTTCTCAAGGTCAACAACTTGTGACCAAATTAGTGACTGCTCCTGTAGCTTGT
  GGGGCAGTCATGGTACCTAGTACTATGCTTATGGGCCAGGTGGTGACTGCATATCCTACT
  TTTGCTACACAACAGCAACAGTCACAGACATTGTCAGTAACGCAGCAGCAGCAGCAGCAG
  AGCTCCCAGGAGCAGCAGCTCACTTCAGTTCAGCAACCATCTCAGGCTCAGCTGACCCAG
  CCACCGCAACAATTTTTACAGACTTCTAGGTTGCTCCATGGGAATCCCTCAACTCAACTC
  ATTCTCTCTGCTGCATTTCCTCTACAACAGAGCACCTTCCCTCAGTCACATCACCAGCAA
  CATCAGTCTCAGCAACAGCAGCAACTCAGCCGGCACAGGACTGACAGCTTGCCCGACCCT
  TCCAAGGTTCAACCACAGTAG
  
  Clone variation with respect to NM_004898.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004898 unedited
  NNTGGTTCGNATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGCACCGC
  AGCGGGAGACCTGGGTGGGGGAGGGAAGAAGCCGGAGCCGCCGCAAGCCACACGAATTCT
  GAGACTTATGGTTGGTCATATAGAAGAGTACCTTGAACCTATAGTTTCCTGAAGAATCAG
  TTTAAAAGATCCAAGGAGTACAAAAGGAGAAGTACAAATGTCTACTACAAGACGAAAACG
  TAGTATGTTATGTTGTTTACCGTAAGCTGTAGTAAAATGAGCTCGATTGTTGACAGAGAT
  GACAGTAGTATTTTTGATGGGTTGGTGGAAGAAGATGACAAGGACAAAGCGAAAAGAGTA
  TCTAGAAACAAATCTGAAAAGAAACGTAGAGATCAATTTAATGTTCTCATTAAAGAACTG
  GGATCCATGCTTCCTGGTAATGCTAGAAAGATGGACAAATCTACTGTTCTGCAGAAAAGC
  ATTGATTTTTTACGAAAACATAAAGAAATCACTGCACAGTCAGATGCTAGTGAAATTCGA
  CAGGACTGGAAACCTACATTCCTTAGTAATGAAGAGTTTACACAATTAATGTTAGAGGCT
  CTTGATGGTTTTTTTTTAGCAATCATGACAGATGGAAGCATAATATATGTGTCTGAGAGT
  GTAACTTCATTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTT
  ATCCCAGAAGGGGAACATTCAGAGGTTTATAAAATACTCTCTACTCATCTGCTGGAAAGT
  GATTCATTAACCCCAGAATATTTNAAAATCAAAAATCAGTTAGAATTCTGTTGTCACATG
  CTGCGAGGNAACATAGACCCAAAGGAGCCATCTACCTATGAATATGNNTAAATTATAGGN
  AAATTNCANATCTTTAACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004898 unedited
  AGCTTGGACCGCGCTCCAATTTANGATCGGTTTTTTTTCTTTTTTTTTTTAAAGCTGGAA
  GGTGTTTCTATTTGGTTTCTTTAAATGCTCTAGATTTGGGATTTTAACTAACAGCTTTGG
  CTATAGAAATGAAGAACAGACACTGCTGAATCTTCATTTGAATGCTCAAGTTTCATGGGG
  CAGGGTGGGGGGCGGGATCGCTTTGCAACCCATTTTTTTGAGAATGCGGAGGAAAACATT
  TCTTCCAGAGGCCAGTGGTGCTAGCTACCTCGGCCCGTGCCCGCGTGCGCGCTGCCCCGT
  CTCTGCGTACCCTGTTCTTTCTCCTCACTCCCCCTTGCACCCTCCTCTCCCCCCCCTCCC
  CCCCCCCCCCCCGTCCCCCCCCCCCCCCCCCCCGCCCTCCCACCTCCCCCGCTCTGTCTT
  TCCCTCGCCTTGACCCTCCTTTCCAATCTTCCCTCCGTATTTCCCTCCCCCACCCCCCCC
  CTGCTCCCCTCCTCGCCTCCCCGTCTCTCCCCTCCCCGTCCGCTCCCCCCCTCCCCCTCC
  TTGGCCCCCCGTCCCCTGTCTCCTCCGCCCTCGCCTAGTGCTCTCCCGCCGCCTCCCGCC
  CCCCCCCCCCCCCCCCCCCCCCCCCACGCGTCCTTCGGGGTGGCCTTTCTCCCCCGTCCC
  GCTCTATCCCACCCCTCCCCCGTCCTTGCCGCCCTCCTGCTCCGCTTCCTCGCCGGCCCC
  CCTTACCTCCTCGCCCTGCGCCGCCCCCGCCCCCTCCCCCCCCCCGTTGCCCCCGCCCCC
  GCCCCATGCCCGTGCCGTCCCGCCAGCTTTCCTTTCACCGATCCCCCCCCCACGTACGTG
  TCGCGCGTCGCCTGCCGCAGCCCCCGAGCGCCTCCCTATCTACTTCACCCCGCGCCCCCC
  TCCCCACACCCCCCCCGACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004898
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004898.2, NP_004889.1
• RefSeq Size: 5801 bp
• RefSeq ORF: 2541 bp
• Locus ID: 9575
• UniProt ID: O15516
• Cytogenetics: 4q12
• Domains: PAS, HLH, PAC
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.