HMGCS2 (NM_005518) Human Untagged Clone

CAT#: SC116704

HMGCS2 (untagged)-Human 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HMGCS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005518 edited
  GCGGCCGCGAATTCGGCACGAGGCTGAACTGCTGGGTTTCTGCTTGCTCCTCTGGAGATG
  CAGCGTCTGTTGACTCCAGTGAAGCGCATTCTGCAACTGACAAGAGCGGTGCAGGAAACC
  TCCCTCACACCTGCTCGCCTGCTCCCAGTAGCCCACCAAAGGTTTTCTACAGCCTCTGCT
  GTCCCCCTGGCCAAAACAGATACTTGGCCAAAGGACGTGGGCATCCTGGCCCTGGAGGTC
  TACTTCCCAGCCCAATATGTGGACCAAACTGACCTGGAGAAGTATAACAATGTGGAAGCA
  GGAAAGTATACAGTGGGCTTGGGCCAGACCCGTATGGGCTTCTGCTCAGTCCAAGAGGAC
  ATCAACTCCCTGTGCCTGACGGTGGTGCAACGGCTGATGGAGCGCATACAGCTCCCATGG
  GACTCTGTGGGCAGGCTGGAAGTAGGCACTGAGACCATCATTGACAAGTCCAAAGCTGTC
  AAAACAGTGCTCATGGAACTCTTCCAGGATTCAGGCAATACTGATATTGAGGGCATAGAT
  ACCACCAATGCCTGCTACGGTGGTACTGCCTCCCTCTTCAATGCTGCCAACTGGATGGAG
  TCCAGTTCCTGGGATGGTCGTTATGCCATGGTGGTCTGTGGAGACATTGCCGTCTATCCC
  AGTGGTAATGCTCGTCCCACAGGTGGGGCCGGAGCTGTGGCTATGCTGATTGGGCCCAAG
  GCCCCTCTGGCCCTGGAGCGAGGGCTGAGGGGAACCCATATGGAGAATGTGTATGACTTC
  TACAAACCAAATTTGGCCTCGGAGTACCCAATAGTGGATGGGAAGCTTTCCATCCAGTGC
  TACTTGCGGGCCTTGGATCGATGTTACACATCATACCGTAAAAAAATCCAGAATCAGTGG
  AAGCAAGCTGGCAGCGATCGACCCTTCACCCTTGACGATTTACAGTACATGATCTTTCAT
  ACACCCTTTTGCAAGATGGTCCAGAAGTCTCTGGCTCGCCTGATGTTCAATGACTTCCTG
  TCAGCCAGCAGTGACACACAAACCAGCTTATATAAGGGGCTGGAGGCTTTCGGGGGGCTA
  AAGCTGGAAGACACCTACACCAACAAGGACCTGGATAAAGCACTTCTAAAGGCCTCTCAG
  GACATGTTCGACAAGAAAACCAAGGCTTCCCTTTACCTCTCCACTCACAATGGGAACATG
  TACACCTCATCCCTGTACGGGTGCCTGGCCTCGCTTCTGTCCCACCACTCTGCCCAAGAA
  CTGGCTGGCTCCAGGATTGGTGCCTTCTCTTATGGCTCTGGTTTAGCAGCAAGTTTCTTT
  TCATTTCGAGTATCCCAGGATGCTGCTCCAGGCTCTCCCCTGGACAAGTTGGTGTCCAGC
  ACATCAGACCTGCCAAAACGCCTAGCCTCCCGAAAGTGTGTGTCTCCTGAGGAGTTCACA
  GAAATAATGAACCAAAGAGAGCAATTCTACCATAAGGTGAATTTCTCCCCACCTGGTGAC
  ACAAACAGCCTTTTCCCAGGTACTTGGTACCTGGAGCGAGTGGACGAGCAGCATCGCCGA
  AAGTATGCCCGGCGTCCCGTCTAAAGGTGTTCTGCAGATCCATGGAAAGCTTCCTGGGAA
  ACGTATGCTAGCAGAGCTTCTCCCCGTGAATCATATTTTTAAGATCCCACTCTTAGCTGG
  TAAATGAATTTGAATCGACATAGTAGCCCCATAAGCATCAGCCCTGTAGAGTGAGGAGCC
  ATCTCTAGCGGGCCCTTCATTCCTCTCCATGCTGCAATCACTGTCCTGGGCTTATGGTGC
  TATGGACTAGGGGTCCTTTGTGAAAGAGCAAGATGGAGCAATGGAGAGAAGACCTCTTCC
  TGAATCACTGGACTCCAGAAATGTGCATGCAGATCAGCTGTTGCCTTCAAGATCCAGATA
  AACTTTCCTGTCATGTGTTAGAACTTTATTATTATTAATATTGTTAAACTTCTGTGCTGT
  TCCTGTGAATCTCCAAATTTTGTACCTTGTTTTAAGGCTAATATATAGCAATTAAAAAGA
  GAGAAAGAGGAAAAAAAAAAAAAAAAAACTCGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005518 unedited
  TTGTTTCANNATATTTGTNNAATACGACNCACTATAGGGCGGCCGCGAATTCGGCACGAG
  GCTGAACTGCTGGGTTTCTGCTTGCTCCTCTGGAGATGCAGCGTCTGTTGACTCCAGTGA
  AGCGCATTCTGCAACTGACAAGAGCGGTGCAGGAAACCTCCCTCACACCTGCTCGCCTGC
  TCCCAGTAGCCCACCAAAGGTTTTCTACAGCCTCTGCTGTCCCCCTGGCCAAAACAGATA
  CTTGGCCAAAGGACGTGGGCATCCTGGCCCTGGAGGTCTACTTCCCAGCCCAATATGTGG
  ACCAAACTGACCTGGAGAAGTATAACAATGTGGAAGCAGGAAAGTATACAGTGGGCTTGG
  GCCAGACCCGTATGGGCTTCTGCTCAGTCCAAGAGGACATCAACTCCCTGTGCCTGACGG
  TGGTGCAACGGCTGATGGAGCGCATACAGCTCCCATGGGACTCTGTGGGCAGGCTGGAAG
  TAGGCACTGAGACCATCATTGACAAGTCCAAAGCTGTCAAAACAGTGCTCATGGAACTCT
  TCCAGGATTCAGGCAATACTGATATTGAGGGCATAGATACCACCAATGCCTGCTACGGTG
  GTACTGCCTCCCTCTTCAATGCTGCCAACTGGATGGAGTCCAGTTCCTGNGATGGTCGTT
  ATGCCATGGTGGTCTGTGGAGACATTGCCGTCTATCCCAGTGGTAATGCTCGTCCCACAG
  GTGGGGCCGGAGCTGTGGCTATGCTGATTGGGCCCAAAGCCCCTCTGGCCCTGGAGCGAG
  GGCTGAAGGGAACCCATATGGAGAATGTGTATGACTTCTACAAACCAAATTTGGCCTCGG
  AGTACCCCATAGTGGATGGGAAGCTTTTCATCCAATGCTACCTGCCGGCCTTGGTATCGA
  GTTACACATATACCGGAAAAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005518 unedited
  CGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCCTCTTTCTCTCTTTTTAATT
  GCTATATATTAGCCTTAAAACAAGGTACAAAATTTGGAGATTCACAGGAACAGCACAGAA
  GTTTAACAATATTAATAATAATAAAGTTCTAACACATGACAGGAAAGTTTATCTGGATCT
  TGAAGGCAACAGCTGATCTGCATGCACATTTCTGGAGTCCAGTGATTCAGGAAGAGGTCT
  TCTCTCCATTGCTCCATCTTGCTCTTTCACAAAGGACCCCTAGTCCATAGCACCATAAGC
  CCAGGACAGTGATTGCAGCATGGAGAGGAATGAAGGGCCCGCTAGAGATGGCTCCTCACT
  CTACAGGGCTGATGCTTATGGGGCTACTATGTCGATTCAAATTCATTTACCAGCTAAGAG
  TGGGATCTTAAAAATATGATTCACGGGGAGAAGCTCTGCTAGCATACGTTCCCCAGGAAG
  CTTTCCATGGATCTGCAAAACACCTTTAGACGGGACGCCGGGCATACTTTCGGCGATGCT
  GTTCGTCCACTCGCTCCAGGTACCAAGTACCTGCGAAAAGGCTGTTTGTGCCACCACGTG
  GGCACAAATCCACCTTATGGTACAATTGCCCTCTTTGCGTCATTATTCCTGTGAACCCCT
  TACGAGACACCCACTTTTGGGAAGGCTCCGCGCCTCGCCAGGTCTCGAGTGCTTGGACAC
  CAACTCGATCAGGGGAGACCCTGGATCCATTCCTTGGCAACTTTAATGAACACAACCCCC
  CGTTCATCCCCCCACCATATATCCCTTTTCTCTGTCCCCCCCCCCCGCCTTATTTCCCCA
  TTATTTTGTCCTCTCTCCCCCGTTGAGCTTTCTTTCACCCTGCCCCCCCGTTCCGCTATT
  CCCCCCCCCCACTCTGCTTTTTGGCCACCCCCCCCCCCTCATTTTCCCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005518
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005518.2, NP_005509.1
• RefSeq Size: 2082 bp
• RefSeq ORF: 1527 bp
• Locus ID: 3158
• UniProt ID: P54868
• Cytogenetics: 1p12
• Domains: HMG_CoA_synt
• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Metabolic pathways, PPAR signaling pathway, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Valine, leucine and isoleucine degradation
• Gene Summary: The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) encodes the longer isoform (1).