AKR1D1 (NM_005989) Human Untagged Clone

CAT#: SC116410

AKR1D1 (untagged)-Human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AKR1D1
• Synonyms: 3o5bred; CBAS2; SRD5B1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005989 edited
  ATGGATCTCAGTGCTGCAAGTCACCGCATACCTCTAAGTGATGGAAACAGCATTCCCATC
  ATCGGACTTGGTACCTACTCAGAACCTAAATCGACCCCTAAGGGAGCCTGTGCAACATCG
  GTGAAGGTTGCTATTGACACAGGGTACCGACATATTGATGGGGCCTACATCTACCAAAAT
  GAACACGAAGTTGGGGAGGCCATCAGGGAGAAGATAGCAGAAGGAAAGGTGCGGAGGGAA
  GATATCTTCTACTGTGGAAAGCTATGGGCTACAAATCATGTCCCAGAGATGGTCCGCCCA
  ACCCTGGAGAGGACACTCAGGGTCCTCCAGCTAGATTATGTGGATCTTTACATCATTGAA
  GTACCCATGGCCTTTAAGCCAGGAGATGAAATATACCCTAGAGATGAGAATGGCAAATGG
  TTATATCACAAGTCAAATCTGTGTGCCACTTGGGAGGCGATGGAAGCTTGCAAAGACGCT
  GGCTTGGTGAAATCCCTGGGAGTGTCCAATTTTAACCGCAGGCAGCTGGAGCTCATCCTG
  AACAAGCCAGGACTCAAACACAAGCCAGTCAGCAACCAGGTTGAGTGCCATCCGTATTTC
  ACCCAGCCAAAACTCTTGAAATTTTGCCAACAACATGACATTGTCATTACTGCATATAGC
  CCTTTGGGGACCAGTAGGAATCCAATCTGGGTGAATGTTTCTTCTCCACCTTTGTTAAAG
  GATGCACTTCTAAACTCATTGGGGAAAAGGTACAATAAGACAGCAGCTCAAATTGTTTTG
  CGTTTCAACATCCAGCGAGGGGTGGTTGTCATTCCTAAAAGCTTTAATCTTGAAAGGATC
  AAAGAAAATTTTCAGATCTTTGACTTTTCTCTCACTGAAGAAGAAATGAAGGACATTGAA
  GCCTTGAATAAAAATGTCCGCTTTGTAGAATTGCTCATGTGGCGCGATCATCCTGAATAC
  CCATTTCATGATGAATACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005989 unedited
  NNGGTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGCACAATGG
  ATCTCAGTGCTGCAAGTCACCGCATACCTCTAAGTGATGGAAACAGCATTCCCATCATCG
  GACTTGGTACCTACTCAGAACCTAAATCGACCCCTAAGGGAGCCTGTGCAACATCGGTGA
  AGGTTGCTATTGACACAGGGTACCGACATATTGATGGGGCCTACATCTACCAAAATGAAC
  ACGAAGTTGGGGAGGCCATCAGGGAGAAGATAGCAGAAGGAAAGGTGCGGAGGGAAGATA
  TCTTCTACTGTGGAAAGCTATGGGCTACAAATCATGTCCCAGAGATGGTCCGCCCAACCC
  TGGAGAGGACACTCAGGGTCCTCCAGCTAGATTATGTGGATCTTTACATCATTGAAGTAC
  CCATGGCCTTTAAGCCAGGAGATGAAATATACCCTAGAGATGAGAATGGCAAATGGTTAT
  ATCACAAGTCAAATCTGTGTGCCACTTGGGAGGCGATGGAAGCTTGCAAAGACGCTGGCT
  TGGTGAAATCCCTGGGAGTGTCCAATTTTAACCGCAGGCAGCTGGAGCTCATCCTGAACA
  AGCCAGGACTCAAACACAAGCCAGTCAGCAACCAGGTTGAGTGCCATCCGTATTTCACCC
  AGCCAAAACTCTTGAAATTTTGCCAACAACATGACATTGTCATTACTGCATATAGCCCTT
  TGGGGACCAGTANGAATCCAATCTGGGTGAATGTTTCTTCTCCACCTTTGTTAAAGGATG
  CACTTCTAAACTCATTGGGGAAAAGGTACAATAAGACAGCAGCTCANATTGTTTTTGCGT
  TTCAACATCCAGCGAGGGGGTGGTTGTCATTCCTAAAAGCTTTAATCTTGAAAGGATCAA
  AGAAAATTTTCAGATCTTTGACTTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005989 unedited
  TTGACGCATTGGCGATTGGCACCTTTCCAGGTCCAGTACAGTCACTGGGGAATGGGTCAC
  AGGGCTGCCCCCCGTGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTATATTC
  GAGTTTTTTTTTTTTTTTTTTTTTTTTTGATGCAACAAATCATTATACTTTATTATTTCT
  TGTGAACAAAATATTTTCCAGAGGTCAGTGTTATAAGTAATAATATTGGAATCAGTGAAA
  ACAATCAGTATAGACCAAATTTTTTAAAATGATAATTTCTCAAAAATATATGTCTTGAGG
  TCATTGAACATTATCTATAAGCAACTTATCTTGGGAAAACATAATTTTTGAAGAACTCTC
  TCATCCTATGACAGTATGACAGTGACCTAATGTGAATGAATAAAATATATTTCTTTTCTT
  TTTTTTTTTGTTTTTGAGACAGAGTCTCGCTCTTTCACCCAGGCCGGACTGCAGTGGCGC
  TATCTCGGCTCACTGCAAGCTCCTCCTCCCGGGTTCATGCCATTCTTCTGCCTCAACCTC
  CTGAGTAGCTGGGATTACAGGCCCCCGCCACCGCGCCTGGCTATTTTTTTTTAAAAAAAA
  AAAAAAACCAGNNTTNACACCTTTTTAGCCCAGAATGTCTTCGTTCTCCTGACCTCCTGA
  TCTGCCCACCTTGGCCTCCCCAAGTGCTTGTATTTTTGGCATGAGCCCCCCGCCCCCGGC
  CAAATAAATATTTTTCTGTAGTTGAATTGAAAATAGATTATTCTCTTTTGCTGAATTTCA
  ATAATCTTATTCACTCTTTAAATTGTCCCTCCCGGAAAATTTCCAAATTTCTTTTTAACT
  TGCTTTCTCAAAATTCATTTAAAATTTCCAATTTCTTTTAGCTTTTCCAATTACACTTTT
  AAATTCTTAAGTCCATTATTCTTAAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005989
• Insert Size: 981 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005989.2, NP_005980.1
• RefSeq Size: 2692 bp
• RefSeq ORF: 981 bp
• Locus ID: 6718
• UniProt ID: P51857
• Cytogenetics: 7q33
• Domains: aldo_ket_red
• Protein Families: Druggable Genome
• Protein Pathways: Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways, Primary bile acid biosynthesis
• Gene Summary: The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.