AHCYL1 (NM_006621) Human Untagged Clone

CAT#: SC115987

AHCYL1 (untagged) - Homo sapiens adenosylhomocysteinase-like 1 (AHCYL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AHCYL1
• Synonyms: DCAL; IRBIT; PPP1R78; PRO0233; XPVKONA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115987 sequence for NM_006621 edited (data generated by NextGen Sequencing)
  ATGTCGATGCCTGACGCGATGCCGCTGCCCGGGGTCGGGGAGGAGCTGAAGCAGGCCAAG
  GAGATCGAGGACGCCGAGAAGTACTCCTTCATGGCCACCGTCACCAAGGCGCCCAAGAAG
  CAAATCCAGTTTGCTGATGACATGCAGGAGTTCACCAAATTCCCCACCAAAACTGGCCGA
  AGATCTTTGTCTCGCTCGATCTCACAGTCCTCCACTGACAGCTACAGTTCAGCTGCATCC
  TACACAGATAGCTCTGATGATGAGGTTTCTCCCCGAGAGAAGCAGCAAACCAACTCCAAG
  GGCAGCAGCAATTTCTGTGTGAAGAACATCAAGCAGGCAGAATTTGGACGCCGGGAGATT
  GAGATTGCAGAGCAAGACATGTCTGCTCTGATTTCACTCAGGAAACGTGCTCAGGGGGAG
  AAGCCCTTGGCTGGTGCTAAAATAGTGGGCTGTACACACATCACAGCCCAGACAGCGGTG
  TTGATTGAGACACTCTGTGCCCTGGGGGCTCAGTGCCGCTGGTCTGCTTGTAACATCTAC
  TCAACTCAGAATGAAGTAGCTGCAGCACTGGCTGAGGCTGGAGTTGCAGTGTTCGCTTGG
  AAGGGCGAGTCAGAAGATGACTTCTGGTGGTGTATTGACCGCTGTGTGAACATGGATGGG
  TGGCAGGCCAACATGATCCTGGATGATGGGGGAGACTTAACCCACTGGGTTTATAAGAAG
  TATCCAAACGTGTTTAAGAAGATCCGAGGCATTGTGGAAGAGAGCGTGACTGGTGTTCAC
  AGGCTGTATCAGCTCTCCAAAGCTGGGAAGCTCTGTGTTCCGGCCATGAACGTCAATGAT
  TCTGTTACCAAACAGAAGTTTGATAACTTGTACTGCTGCCGAGAATCCATTTTGGATGGC
  CTGAAGAGGACCACAGATGTGATGTTTGGTGGGAAACAAGTGGTGGTGTGTGGCTATGGT
  GAGGTAGGCAAGGGCTGCTGTGCTGCTCTCAAAGCTCTTGGAGCAATTGTCTACATTACC
  GAAATCGACCCCATCTGTGCTCTGCAGGCCTGTATGGATGGGTTCAGGGTGGTAAAGCTA
  AATGAAGTCATCCGGCAAGTCGATGTCGTAATAACTTGCACAGGAAATAAGAATGTAGTG
  ACACGGGAGCACTTGGATCGCATGAAAAACAGTTGTATCGTATGCAATATGGGCCACTCC
  AACACAGAAATCGATGTGACCAGCCTCCGCACTCCGGAGCTGACGTGGGAGCGAGTACGT
  TCTCAGGTGGACCATGTCATCTGGCCAGATGGCAAACGAGTTGTCCTCCTGGCAGAGGGT
  CGTCTACTCAATTTGAGCTGCTCCACAGTTCCCACCTTTGTTCTGTCCATCACAGCCACA
  ACACAGGCTTTGGCACTGATAGAACTCTATAATGCACCCGAGGGGCGATACAAGCAGGAT
  GTGTACTTGCTTCCTAAGAAAATGGATGAATACGTTGCCAGCTTGCATCTGCCATCATTT
  GATGCCCACCTTACAGAGCTGACAGATGACCAAGCAAAATATCTGGGACTCAACAAAAAT
  GGGCCATTCAAACCTAATTATTACAGATACTAA
  
  Clone variation with respect to NM_006621.5
  1053 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006621 unedited
  GGCCGCGCGGGCAGGCGGGCGGGCGCCAGAGTGGGGANNGATGCGGGGGCGGCGGGTCAG
  CCGCTTGGCCGGGCCGGCCGGGGAATGTCGATGCCTGACGCGATGCCGCTGCCCGGGGTC
  GGGGAGGAGCTGAAGCAGGCCAAGGAGATCGAGGACGCCGAGATGTACTCCTTCATGGCC
  ACCGTCACCAAGGCGCCCAAGAAGCAAATCCAGTTTGCTGATGACATGCAGGAGTTCACC
  AAATTCCCCACCAAAACTGGCCGAAGATCTTTGTCTCGCTCGATCTCACAGTCCTCCACT
  GACAGCTACAGTTCAGCTGCATCCTACACAGATAGCTCTGATGATGAGGTTTCTCCCCGA
  GAGAAGCAGCAAACCAACTCCAAGGGCAGCAGCAATTTCTGTGTGAAGAACATCAAGCAG
  GCAGAATTTGGACGCCGGGAGATTGAGATTGCAGAGCAAGACATGTCTGCTCTGATTTCA
  CTCAGGGAAACGTGCTCAGGGGGAGAAAGCCCTTGGCTGGTGCTAAAATAGTGGGCTGTA
  CACACATCACAGCCCAGACAGCGGTGTTGATTGAGACACTCTTGTGCCCTGGGGGGCTCA
  NTGCCCGCTTGGTCTGCTTGTAACATCTACTCAACTCAGAATGAANTAGCTGCAGCACTG
  GCTGAGGCTTGAGTTTGCAGTGGTTCNTTGGGAAGGGCGAGTCAAAAAANGACTTNTGGG
  GGGNGTATTGACCCCCTGGGTGACATGGATNGGGGGGGCAGGCCACCATGATCCTGGATG
  AGGGGGGGGAAAATTAACCCNCTGGGGTTTTAAAAAATTCCAAACGGGTTAAAAAAAACC
  AGGCTTTTGGNAAAAAAGGGNNNNGGGGTTANAAGCTTTTTAACTTTCANTNGAAAAANT
  TTNTTTCCGCCCNANCAAAAAATTTTTTCCAAAAAAAATTTTAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006621 unedited
  TGGGGGATGGGCAACTTCCCAGGTCCAGGCAAAGGCCTGGGGGAGGGTCACAGGGCATGC
  CACACCGGGCATCTGTTCAGGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTT
  TTTTTTTTTTTTTTTTGGACAACAGAAATAAATCCTAGAGGACAAAATTAAACTCAATAG
  AGTGTAGTCTAGTTAAAAACTCGAAAAAGAGCAAGTCTGGTGGGAGTGGAGGAAGGGCTA
  TACTATAAATCCAAGTGGGCCTCCTGATCTTAACAAGCCATGCTCATTATACACATCTCT
  GAACTGGACATACCACCTTTACGCAGGAAACAGGGCTTGGAACTTCTAAGGGAAATTAAC
  ATGCACCACCCACATCTAACCTACCTGCCGGGTAGGTACCATCCCTGCTTCTCTGAAGAA
  GTGAAAGAGCACTGATTTCAGCAGCTAAGAAATGGGCTCTTTTAAGGCGATTTAGACATT
  GCAGATTGTTCCACAGAGAAGGGACCTTAGCATTTCCTGTTAATAAGGTACCTAATTAAG
  ACTAAAGTCCCCAGAAATGGTATAGAAAGAATCACCTTTCTGGGCTGGGAGAGTAAAATC
  CTTCTTTAGTGACTAGAGTGAGGAACCCTGAACTTTTATTTCATTTAAAGAGCCATGAAA
  CATGTTCCTGGGTGGGTCTGCAANACTTGGGATATTGAGATGAAAAAACAGGAATGAGAT
  TATAGGAAAAAAATCCAGAGGAAAGGTTAAGAACAAAATCAAGGTATCTTAAAAAATTTC
  TTTAAATGGGTGGGTCAAGTGGACTGGCCCTGGGAAAATGGGCCATTAGATCTGTAAAAT
  AGGGGTGAAGGCCCATTTTTGTGAGACCAAATTTCGTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006621
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006621.3, NP_006612.2
• RefSeq Size: 2677 bp
• RefSeq ORF: 1593 bp
• Locus ID: 10768
• UniProt ID: O43865
• Cytogenetics: 1p13.3
• Domains: AdoHcyase
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
• Gene Summary: The protein encoded by this gene interacts with inositol 1,4,5-trisphosphate receptor, type 1 and may be involved in the conversion of S-adenosyl-L-homocysteine to L-homocysteine and adenosine. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.