Complement Component 6 (C6) (NM_000065) Human Untagged Clone

CAT#: SC115886

C6 (untagged)-Human complement component 6 (C6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Complement Component 6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115886 sequence for NM_000065 edited (data generated by NextGen Sequencing)
  ATGGCCAGACGCTCTGTCTTGTACTTCATCCTGCTGAATGCTCTGATCAACAAGGGCCAA
  GCCTGCTTCTGTGATCACTATGCATGGACTCAGTGGACCAGCTGCTCAAAAACTTGCAAT
  TCTGGAACCCAGAGCAGACACAGACAAATAGTAGTAGATAAGTACTACCAGGAAAACTTT
  TGTGAACAGATTTGCAGCAAGCAGGAGACTAGAGAATGTAACTGGCAAAGATGCCCCATC
  AACTGCCTCCTGGGAGATTTTGGACCATGGTCAGACTGTGACCCTTGTATTGAAAAACAG
  TCTAAAGTTAGATCTGTCTTGCGTCCCAGTCAGTTTGGGGGACAGCCATGCACTGAGCCT
  CTGGTAGCCTTTCAACCATGCATTCCATCTAAGCTCTGCAAAATTGAAGAGGCTGACTGC
  AAGAATAAATTTCGCTGTGACAGTGGCCGCTGCATTGCCAGAAAGTTAGAATGCAATGGA
  GAAAATGACTGTGGAGACAATTCAGATGAAAGGGACTGTGGGAGGACAAAGGCAGTATGC
  ACACGGAAGTATAATCCCATCCCTAGTGTACAGTTGATGGGCAATGGGTTTCATTTTCTG
  GCAGGAGAGCCCAGAGGAGAAGTCCTTGATAACTCTTTCACTGGAGGAATATGTAAAACT
  GTCAAAAGCAGTAGGACAAGTAATCCATACCGTGTTCCGGCCAATCTGGAAAATGTCGGC
  TTTGAGGTACAAACTGCAGAAGATGACTTGAAAACAGATTTCTACAAGGATTTAACTTCT
  CTTGGACACAATGAAAATCAACAAGGCTCATTCTCAAGTCAGGGGGGGAGCTCTTTCAGT
  GTACCAATTTTTTATTCCTCAAAGAGAAGTGAAAATATCAACCATAATTCTGCCTTCAAA
  CAAGCCATTCAAGCCTCTCACAAAAAGGATTCTAGTTTTATTAGGATCCATAAAGTGATG
  AAAGTCTTAAACTTCACAACGAAAGCTAAAGATCTGCACCTTTCTGATGTCTTTTTGAAA
  GCACTTAACCATCTGCCTCTAGAATACAACTCTGCTTTGTACAGCCGAATATTCGATGAC
  TTTGGGACTCATTACTTCACCTCTGGCTCCCTGGGAGGCGTGTATGACCTTCTCTATCAG
  TTTAGCAGTGAGGAACTAAAGAACTCAGGTTTAACCGAGGAAGAAGCCAAACACTGTGTC
  AGGATTGAAACAAAGAAACGCGTTTTATTTGCTAAGAAAACAAAAGTGGAACATAGGTGC
  ACCACCAACAAGCTGTCAGAGAAACATGAAGGTTCATTTATACAGGGAGCAGAGAAATCC
  ATATCCCTGATTCGAGGTGGAAGGAGTGAATATGGAGCAGCTTTGGCATGGGAGAAAGGG
  AGCTCTGGTCTGGAGGAGAAGACATTTTCTGAGTGGTTAGAATCAGTGAAGGAAAATCCT
  GCTGTGATTGACTTTGAGCTTGCCCCCATCGTGGACTTGGTAAGAAACATCCCCTGTGCA
  GTGACAAAACGGAACAACCTCAGGAAAGCTTTGCAAGAGTATGCAGCCAAGTTCGATCCT
  TGCCAGTGTGCTCCATGCCCTAATAATGGCCGACCCACCCTCTCAGGGACTGAATGTCTG
  TGTGTGTGTCAGAGTGGCACCTATGGTGAGAACTGTGAGAAACAGTCTCCAGATTATAAA
  TCCAATGCAGTAGACGGACAGTGGGGTTGTTGGTCTTCCTGGAGTACCTGTGATGCTACT
  TATAAGAGATCGAGAACCCGAGAATGCAATAATCCTGCCCCCCAACGAGGAGGGAAACGC
  TGTGAGGGGGAGAAGCGACAAGAGGAAGACTGCACATTTTCAATCATGGAAAACAATGGA
  CAACCATGTATCAATGATGATGAAGAAATGAAAGAGGTCGATCTTCCTGAGATAGAAGCA
  GATTCCGGGTGTCCTCAGCCAGTTCCTCCAGAAAATGGATTTATCCGGAATGAAAAGCAA
  CTATACTTGGTTGGAGAAGATGTTGAAATTTCATGCCTTACTGGCTTTGAAACTGTTGGA
  TACCAGTACTTCAGATGCTTACCAGACGGGACCTGGAGACAAGGGGATGTGGAATGCCAA
  CGGACGGAGTGCATCAAGCCAGTTGTGCAGGAAGTCCTGACAATTACACCATTTCAGAGA
  TTGTATAGAATTGGTGAATCCATTGAGCTAACTTGCCCCAAAGGCTTTGTTGTTGCTGGG
  CCATCAAGGTACACATGCCAGGGGAATTCCTGGACACCACCCATTTCAAACTCTCTCACC
  TGTGAAAAAGATACTCTAACAAAATTAAAAGGCCATTGTCAGCTGGGACAGAAACAATCA
  GGATCTGAATGCATTTGTATGTCTCCAGAAGAAGACTGTAGCCATCATTCAGAAGATCTC
  TGTGTGTTTGACACAGACTCCAACGATTACTTTACTTCACCCGCTTGTAAGTTTTTGGCT
  GAGAAATGTTTAAATAATCAGCAACTCCATTTTCTACATATTGGTTCCTGCCAAGACGGC
  CGCCAGTTAGAATGGGGTCTTGAAAGGACAAGACTTTCATCCAACAGCACAAAGAAAGAA
  TCCTGTGGCTATGACACCTGCTATGACTGGGAAAAATGTTCAGCCTCCACTTCCAAATGT
  GTCTGCCTATTGCCCCCACAGTGCTTCAAGGGTGGAAACCAACTCTACTGTGTCAAAATG
  GGATCATCAACAAGTGAGAAAACATTGAACATCTGTGAAGTGGGAACTATAAGATGTGCA
  AACAGGAAGATGGAAATACTGCATCCTGGAAAGTGTTTGGCCTAG
  
  Clone variation with respect to NM_000065.2
  356 c=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000065 unedited
  CTAGATTTTGTATCCGACTTACTATNAGGNCGGCACGCGCAATTCGGCACGAGGCATTGC
  CTTGTGTTAGCTAGCAATAAGAAAAGAAGCTTTGTTTGGATTAACATATATACCCTCTTC
  ATTCTGCATACCTATTTTTTCCCCAATAATTTGCAGCTTATGTCCGAGGACACCACAAAC
  TCTGCTTAAAGGGCCTGGAGGCTCTCAAGGCATGGCCAGACGCTCTGTCTTGTACTTCAT
  CCTGCTGAATGCTCTGATCAACAAGGGCCAAGCCTGCTTCTGTGATCACTATGCATGGAC
  TCAGTGGACCAGCTGCTCAAAAACTTGCAATTCTGGAACCCAGAGCAGACACAGACAAAT
  AGTAGTAGATAAGTACTACCAGGAAAACTTTTGTGAACAGATTTGCAGCAAGCAGGAGAC
  TAGAGAATGTAACTGGCAAAGATGCCCCATCAACTGCCTCCTGGGAGATTTTGGACCATG
  GTCAGACTGTGACCCTTGTATTGAAAAACAGTCTAAAGTTAGATCTGTCTTGCGTCCCAG
  TCAGTTTGGGGGACAGCCATGCACTGAGCCTCTGGTAGCCTTTCAACCATGCATTCCATC
  TAAGCTCTGCAAAATTGAAGAGGCTGACTGCAAGAATAAATTTCGCTGTGACAGTGGCCG
  CTGCATTGCCAGATAGTTAGAATGCAATGGAGAAAATGACTGTGGAGACCATTCAGATGA
  TAGGGACTGTGGGAGGACATAGGCAGTATGCACACGGAAGTATAATCCCATCCCTAGTGT
  ACAGTTGATGGGCAATGGNGTTCATTTTCTGGCAAGAGAGCCCAGAAGAGAAGTCCTTGA
  TACTCTTNCCTGGNAGAATATGTAAACTGTCAAAGCAGTAGGACAAGTAATCATACCGTG
  TNCCGCCATCTGNAAATGTCGCTTNGAGTACAACTGCAGAGAGACTGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000065 unedited
  TTCTATGNACCGCGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTTCAGAGTTCT
  GGAGATTTCTGTTTATTAACTCATTGAAAAAAATATGGGTTTTATTCTTATTTCTAATTG
  TGAGAATGCTTAATGTCACAGGCTACATAAGGGCCTCAAAAGTCACATTATTTTTGTACA
  ATGTGAACAGGAGAATTTACGAGACTGCTGTGGAAGTTGGTACCTAGGGGTATGCTACAG
  GGCGTCATGAGCTGGAACTGAATAAGACATGCCCAAACAGGAGAGTCAGGGGAGAATAAT
  GATCTCAAACTAACAGAAAATAATTTTTGTCAGTAACTTTGAGCATGCCAGTCTGCTGTT
  TGTGCAAGAATTCTCATTTGTAGGAGTTGGTTCTTCGGGATGGTAAATCTGTTCATTGTG
  CTGGGCCTAGCAGTAATTGTGCTAGGCCAAACACTTTCCAGGATGCAGTATTTCCATCTT
  CCTGTTTGCACATCTTATAGTTCCCACTTCACAGATGTTCAATGTTTTCTCACTTGTTGA
  TGATCCCATTTTGACACAGTAGAGTTGGTTTCCACCCTTGAAGCACTGTGGGGGCAATAG
  GCAGACACATTTGGAAGTGGAGGCTGAACATTNTTCCCAGTCATAGCAGGTGTCATAGCC
  ACAGGATTCTTTCTTTGTGCTGTTGGATGAAAGTCTTGTCCTTTCAGACCCCATTCTAAC
  TGGNCGCCGTCTTGGCAGGAACCCATATGTAGAANATGGGAGTGCTGATTATTTAAACAT
  TNCTCACCAAAAACTTTACAGCGGGTGAAGTAAAGTATCGTTGGAGTCTGTGTCAAACCA
  CAGAGATCTTCTGATGATGGCTACAGTCTTCTTCTGGAGAATACAAAGCATTCGATCCTG
  ANTGTTCTGTCCAGCTGACATGGCCTTTTAANTTGTAGATATCTTTTACAGTGAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000065
• Insert Size: 3110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000065.1, NP_000056.1
• RefSeq Size: 3551 bp
• RefSeq ORF: 2805 bp
• Locus ID: 729
• UniProt ID: P13671
• Cytogenetics: 5p13.1
• Domains: CCP, tsp_1, MACPF, ldl_recept_a, kazal, FIMAC
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• Gene Summary: This gene encodes a component of the complement cascade. The encoded protein is part of the membrane attack complex that can be incorporated into the cell membrane and cause cell lysis. Mutations in this gene are associated with complement component-6 deficiency. Transcript variants encoding the same protein have been described.[provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.