Frizzled 10 (FZD10) (NM_007197) Human Untagged Clone

CAT#: SC115678

FZD10 (untagged)-Human frizzled family receptor 10 (FZD10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Frizzled 10
• Synonyms: CD350; FZ-10; Fz10; FzE7; hFz10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007197 edited
  GAATTCGGCACGAGCCAGGCCGGGCGGGCATGGGCGGGGGCCCGAGCAGGGGTGGAGAGC
  CGGGGCCAGCAGCAGCCCGTGCCCGGGAGCGGCGGCGCTGAGGGGCGCGGAGCTCCCCGC
  GAGGACACGTCCAACGCCAGCATGCAGCGCCCGGGCCCCCGCCTGTGGCTGGTCCTGCAG
  GTGATGGGCTCGTGCGCCGCCATCAGCTCCATGGACATGGAGCGCCCGGGCGACGGCAAA
  TGCCAGCCCATCGAGATCCCGATGTGCAAGGACATCGGCTACAACATGACTCGTATGCCC
  AACCTGATGGGCCACGAGAACCAGCGCGAGGCAGCCATCCAGTTGCACGAGTTCGCGCCG
  CTGGTGGAGTACGGCTGCCACGGCCACCTCCGCTTCTTCCTGTGCTCGCTGTACGCGCCG
  ATGTGCACCGAGCAGGTCTCTACCCCCATCCCCGCCTGCCGGGTCATGTGCGAGCAGGCC
  CGGCTCAAGTGCTCCCCGATTATGGAGCAGTTCAACTTCAAGTGGCCCGACTCCCTGGAC
  TGCCGGAAACTCCCCAACAAGAACGACCCCAACTACCTGTGCATGGAGGCGCCCAACAAC
  GGCTCGGACGAGCCCACCCGGGGCTCGGGCCTGTTCCCGCCGCTGTTCCGGCCGCAGCGG
  CCCCACAGCGCGCAGGAGCACCCGCTGAAGGACGGGGGCCCCGGGCGCGGCGGCTGCGAC
  AACCCGGGCAAGTTCCACCACGTGGAGAAGAGCGCGTCGTGCGCGCCGCTCTGCACGCCC
  GGCGTGGACGTGTACTGGAGCCGCGAGGACAAGCGCTTCGCAGTGGTCTGGCTGGCCATC
  TGGGCGGTGCTGTGCTTCTTCTCCAGCGCCTTCACCGTGCTCACCTTCCTCATCGACCCG
  GCCCGCTTCCGCTACCCCGAGCGCCCCATCATCTTCCTCTCCATGTGCTACTGCGTCTAC
  TCCGTGGGCTACCTCATCCGCCTCTTCGCCGGCGCCGAGAGCATCGCCTGCGACCGGGAC
  AGCGGCCAGCTCTATGTCATCCAGGAGGGACTGGAGAGCACCGGCTGCACGCTGGTCTTC
  CTGGTCCTCTACTACTTCGGCATGGCCAGCTCGCTGTGGTGGGTGGTCCTCACGCTCACC
  TGGTTCCTGGCCGCCGGCAAGAAGTGGGGCCACGAGGCCATCGAAGCCAACAGCAGCTAC
  TTCCACCTGGCAGCCTGGGCCATCCCGGCGGTGAAGACCATCCTGATCCTGGTCATGCGC
  AGGGTGGCGGGGGACGAGCTCACCGGGGTCTGCTACGTGGGCAGCATGGACGTCAACGCG
  CTCACCGGCTTCGTGCTCATTCCCCTGGCCTGCTACCTGGTCATCGGCACGTCCTTCATC
  CTCTCGGGCTTCGTGGCCCTGTTCCACATCCGGAGGGTGATGAAGACGGGCGGCGAGAAC
  ACGGACAAGCTGGAGAAGCTCATGGTGCGTATCGGGCTCTTCTCTGTGCTGTACACCGTG
  CCGGCCACCTGTGTGATCGCCTGCTACTTTTACGAACGCCTCAACATGGATTACTGGAAG
  ATCCTGGCGGCGCAGCACAAGTGCAAAATGAACAACCAGACTAAAACGCTGGACTGCCTG
  ATGGCCGCCTCCATCCCCGCCGTGGAGATCTTCATGGTGAAGATCTTTATGCTGCTGGTG
  GTGGGGATCACCAGCGGGATGTGGATTTGGACCTCCAAGACTCTGCAGTCCTGGCAGCAG
  GTGTGCAGCCGTAGGTTAAAGAAGAAGAGCCGGAGAAAACCGGCCAGCGTGATCACCAGC
  GGTGGGATTTACAAAAAAGCCCAGCATCCCCAGAAAACTCACCACGGGAAATATGAGATC
  CCTGCCCAGTCGCCCACCTGCGTGTGAACAGGGCTGGAGGGAAGGGCACAGGGGCGCCCG
  GAGCTAAGATGTGGTGCTTTTCTTGGTTGTGTTTTTCTTTCTTCTTCTTCTTTTTTTTTT
  TTTATAAAAGCAAAAGAGAAATACATAAAAAAGTGTTTACCCTGAAATTCAGGATGCTGT
  GATACACTGAAAGGAAAAATGTACTTAAAGGGTTTTGTTTTGTTTTGGTTTTCCAGCGAA
  GGGAAGCTCCTCCAGTGAAGTAGCCTCTTGTGTAACTAATTTGTGGTAAAGTAGTTGATT
  CAGCCCTCAGAAGAAAACTTTTGTTTAGAGCCCTCCGTAAATATACATCTGTGTATTTGA
  GTTGGCTTTGCTACCCATTTACAAATAAGAGGACAGATAACTGCTTTGCAAATTCAAGAG
  CCTCCCCTGGGTTAACAAATGAGCCATCCCCAGGGCCCACCCCCAGGAAGGCCACAGTGC
  TGGGCGGCATCCCTGCAGAGGAAAGACAGGACCCGGGGCCCGCCTCACACCCCAGTGGAT
  TTGGAGTTGCTTAAAATAGACTCTGGCCTTCACCAATAGTCTCTCTGCAAGACAGAAACC
  TCCATCAAACCTCACATTTGTGAACTCAAACGATGTGCAATACATTTTTTTCTCTTTCCT
  TGAAAATAAAAAGAGAAACAAGTATTTTGCTATATATAAAGACAACAAAAGAAATCTCCT
  AACAAAAGAACTAAGAGGCCCAGCCCTCAGAAACCTTTCAGTGCTACATTTTGTGGCTTT
  TTAATGGAAACCAAGCCAATGTTATAGACCTTTGGACTGATTTGTGGAAAGGAGGGGGGA
  AGAGGGAGAAGGATCATTCAAAAGTTACCCAAAGGGCTTATTGACTCTTTCTATTGTTAA
  ACAAATGATTTCCACAAACAGATCAGGAAGCACTAGGTTGGCAGAGACACTTTGTCTAGT
  GTATTCTCTTCACAGTGCCAGGAAAGAGTGGTTTCTGCGTGTGTATATTTGTAATATATG
  ATATTTTTCATGCTCCACTATTTTATTAAAAATAAAATATGTTCTTTAAAAAAAAAAAAA
  AAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007197 unedited
  CGGTTTTTTATTTTTTCCGCCGGNCCCGTTCAGATTTGTATACGACTCATATAGGCGGCC
  GCGNAATTCGCACGAGCCAGGCCGGGCGGGCATGGGCGGGGGCCCGAGCAGGGTGGAGAG
  CCGGGGCCAGCAGCAGCCCGTGCCCGGGAGCGGCGGCGCTGAGGGGCGCGGAGCTCCCCG
  CGAGGACACGTCCAACGCCAGCATGCAGCGCCCGGGCCCCCGCCTGTGGCTGGTCCTGCA
  GGTGATGGGCTCGTGCGCCGCCATCAGCTCCATGGACATGGAGCGCCCGGGCGACGGCAA
  ATGCCAGCCCATCGAGATCCCGATGTGCAAGGACATCGGCTACAACATGACTCGTATGCC
  CAACCTGATGGGCCACGAGAACCAGCGCGAGGCAGCCATCCAGTTGCACGAGTTCGCGCC
  GCTGGTGGAGTACGGCTGCCACGGCCACCTCCGCTTCTTCCTGTGCTCGCTGTACGCGCC
  GATGTGCACCGAGCAGGTCTCTACCCCCATCCCCGCCTGCCGGGTCATGTGCGAGCAGGC
  CCGGCTCAAGTGCTCCCCGATTATGGAGCAGTTCAACTTCAAGTGGCCCGACTCCCTGGA
  CTGCCGGNAACTCCCCACAAGAACGACCCCAACTACCTGTGCATGGAGGCGCCCAACACG
  GCTCGGACGAGCCCACCCGGNGCTCGGGCCTGTCCCCGCCGCTGTTCCGGCGCAGCGGCC
  CACAGCGCGCAGGACACCCGCTGAAGGACGGGGGCCCGGNCGCGGCGGCTGCAACAACCC
  GGNNCAGTCCACCACGTGAGAAAACGCGTGTGCGCCCGCTCTGCCGCCCCGGGTGGCAGT
  GTACTGGAGCCGCNAGGACAAGCGCTTCCAATGGTCTGCCTGGCCTCTGGCCGGGGCTGG
  GTTTTTTTTCAGCGCTTACACGGGTTACCTTCTTATGAACCGGCCCGTTTCGGTACCCGA
  GGGCCCANATNTTCCTTTCAGGGCTATGCGGTAACGCGGGGGTACCTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007197 unedited
  TCTTTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTAAAGACA
  TATTTTATTTTTAATAAAATAGTGGAGCATGAAAAAATCATATATTACAAATATACACAC
  GCAGAAACCACTCTTTCCTGGCACTGTGAAGAGAATACACTAGACAAAGTGTCTCTGCCA
  ACCTAGTGCTTCCTGATCTGTTTGTGGAAATCATTTGTTTAACAATAGAAAGAGTCAATA
  AGCCCTTTGGGTAACTTTTGAATGATCCTTCTCCCTCTTCCCCCCTCCTTTCCACAAATC
  AGTCCAAAGGTCTATAACATTGGCTTGGTTTCCATTAAAAAGCCACAAAATGTAGCACTG
  AAAGGTTTCTGAGGGCTGGGCCTCTTAGTTCTTTTGTTAGGAGATTTCTTTTGTTGTCTT
  TATATATAGCAAAATACTTGTTTCTCTTTTTATTTTCAAGGAAAGAGAAAAAAATGTATT
  GCACATCGTTTGAGTTCACAAATGTGAGGTTTGATGGAGGTTTCTGTCTTGCAGAGAGAC
  TATTGGTGAAGGCCAGAGTCTATTTTAAGCAACTCCAAATCCACTGGGGTGTGAGGCGGG
  CCCCGGGTCCTGTCTTTCCTCTGCAGGGATGCCGNCCAGCACTGTGGCCTTCCTGGGGGG
  TGGGCCTGGGATGGCTCATTTGTTAACCCNAGGGGAGCTCTTGAATTTGAAAGCAGTTAT
  CTGTCCTCTTATTTGAAATGGGTAGCAAAGCCAACTCAAAACACAGATGTATATTTACCG
  AGGGCTCAAACAAAAGTTTTCTTCTGAGGGCTGGATCAACTACTTTACCACAAATAGTTA
  CACAGAGGCTACTCACTGGGAGAGCTTCCCTCGCTGAAAACCAAACAAACAAACCCNTTA
  GACATTTTCCTTTCATGTTCACAGCTCTGATTTCAGGTAACCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007197
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007197.2, NP_009128.1
• RefSeq Size: 3260 bp
• RefSeq ORF: 1746 bp
• Locus ID: 11211
• UniProt ID: Q9ULW2
• Cytogenetics: 12q24.33
• Domains: FRI, Frizzled
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. [provided by RefSeq, Jul 2008]