ABCB8 (NM_007188) Human Untagged Clone

CAT#: SC115672

ABCB8 (untagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 8 (ABCB8), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABCB8
• Synonyms: EST328128; M-ABC1; MABC1; MITOSUR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115672 sequence for NM_007188 edited (data generated by NextGen Sequencing)
  ATGCTGGTGCATTTATTTCGGGTCGGGATTCGGGGTGGCCCATTCCCAGGCAGGCTGCTA
  CCGCCCCTCCGCTTCCAGACATTCTCAGCTGTCAGGTACTCTGATGGCTACCGCAGCTCC
  TCCCTCCTCCGGGCCGTGGCCCACCTGCGGTCCCAGCTCTGGGCCCACCTCCCTCGAGCC
  CCCCTAGCTCCCAGATGGAGCCCCTCTGCCTGGTGCTGGGTTGGGGGAGCCCTGCTAGGC
  CCCATGGTACTGAGTAAGCATCCCCACCTCTGCCTTGTGGCCCTGTGTGAGGCAGAAGAG
  GCCCCTCCTGCCAGCTCCACACCCCATGTCGTGGGGTCTCGCTTTAACTGGAAGCTCTTC
  TGGCAGTTTCTGCACCCCCACCTGCTGGTCCTGGGGGTAGCCGTCGTGCTGGCCTTGGGT
  GCGGCACTCGTGAATGTACAGATCCCCCTGCTCCTGGGCCAGCTGGTAGAGGTCGTGGCC
  AAGTACACAAGGGACCACGTAGGGAGTTTCATGACTGAGTCCCAGAATCTCAGCACCCAC
  CTGCTTATCCTCTATGGTGTCCAGGGACTGCTGACCTTCGGGTACCTGGTGCTGCTGTCC
  CACGTTGGCGAGCGCATGGCTGTGGACATGCGGAGGGCCCTCTTCAGCTCCCTGCTCCGA
  CAAGACATCACCTTCTTTGACGCCAATAAGACAGGGCAGCTGGTGAGCCGCTTGACAACT
  GACGTGCAGGAGTTTAAGTCATCCTTCAAGCTTGTCATCTCCCAGGGGCTGCGAAGCTGC
  ACCCAGGTGGCAGGCTGCCTGGTGTCCCTGTCCATGCTGTCGACACGCCTCACGCTGCTG
  CTGATGGTGGCCACACCAGCCCTGATGGGAGTGGGCACCCTGATGGGCTCAGGCCTCCGA
  AAATTGTCTCGCCAGTGTCAGGAGCAGATCGCCAGGGCAATGGGCGTAGCAGACGAGGCC
  CTGGGCAATGTGCGGACTGTGCGAGCCTTCGCCATGGAGCAACGGGAAGAGGAGCGCTAT
  GGGGCAGAGCTGGAAGCCTGCCGCTGCCGGGCAGAGGAGCTGGGCCGCGGCATCGCCTTG
  TTCCAAGGGCTTTCCAACATCGCCTTCAACTGCATGGTCTTGGGTACCCTATTTATTGGG
  GGCTCCCTTGTGGCCGGACAGCAGCTGACAGGGGGAGACCTCATGTCCTTCCTGGTGGCC
  TCCCAGACAGTGCAAAGGTCCATGGCCAACCTCTCTGTCCTGTTTGGGCAGGTGGTCCGG
  GGGCTGAGTGCAGGTGCCCGGGTCTTTGAGTACATGGCCCTGAACCCCTGCATCCCACTG
  TCTGGGGGCTGCTGCGTCCCCAAAGAGCAGCTGCGTGGCTCCGTTACATTTCAGAACGTC
  TGCTTCAGCTACCCCTGCCGCCCCGGCTTCGAGGTGCTGAAAGACTTCACCCTGACGCTG
  CCCCCTGGCAAGATCGTGGCCCTCGTGGGCCAGTCTGGCGGAGGAAAGACCACCGTGGCT
  TCCCTGCTGGAGCGCTTCTACGACCCCACGGCAGGCGTGGTGATGCTGGATGGGCGGGAC
  CTGCGCACCCTTGACCCCTCCTGGCTCCGGGGCCAGGTTGTCGGCTTCATCAGCCAGGAG
  CCCGTCCTGTTTGGGACGACCATCATGGAAAACATCCGCTTTGGGAAGCTGGAAGCTTCC
  GATGAAGAGGTGTACACAGCCGCCCGGGAAGCGAATGCTCACGAGTTCATCACCAGCTTC
  CCCGAGGGCTACAACACGGTCGTCGGTGAACGGGGCACTACCCTGTCTGGGGGCCAGAAG
  CAGCGCCTGGCCATCGCCCGAGCCCTTATCAAGCAGCCCACGGTGCTGATACTGGATGAA
  GCTACCAGCGCGCTGGATGCAGAGTCCGAGCGGGTTGTACAGGAGGCCCTGGACCGGGCC
  AGTGCAGGCCGCACGGTGCTGGTAATTGCCCACCGGCTCAGCACTGTCCGTGGGGCCCAC
  TGCATTGTCGTCATGGCCGATGGCCGTGTCTGGGAGGCTGGGACACATGAAGAGCTCCTG
  AAGAAAGGCGGGCTATACGCCGAGCTCATCCGGAGGCAGGCCCTGGATGCCCCGAGGACA
  GCGGCCCCACCGCCCAAAAAGCCAGAAGGCCCCAGGAGCCACCAGCACAAGTCCTGA
  
  Clone variation with respect to NM_007188.3
  984 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007188 unedited
  NGGGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCAACAT
  AGAGCCCTCAGTGGGATGAGGGTGAAACTGCTATTGCCGGCGGCTCCTGTTTTACCGCGT
  CAGCATGCTGGTGCATTTATTTCGGGTCGGGATTCGGGGTGGCCCATTCCCAGGCAGGCT
  GCTACCGCCCCTCCGCTTCCAGACATTCTCAGCTGTCAGGTACTCTGATGGCTACCGCAG
  CTCCTCCCTCCTCCGGGCCGTGGCCCACCTGCGGTCCCAGCTCTGGGCCCACCTCCCTCG
  AGCCCCCCTAGCTCCCAGATGGAGCCCCTCTGCCTGGTGCTGGGTTGGGGGAGCCCTGCT
  AGGCCCCATGGTACTGAGTAAGCATCCCCACCTCTGCCTTGTGGCCCTGTGTGAGGCAGA
  AGAGGCCCCTCCTGCCAGCTCCACACCCCATGTCGTGGGGTCTCGCTTTAACTGGAAGCT
  CTTCTGGCAGTTTCTGCACCCCCACCTGCTGGTCCTGGGGGTAGCCGTCGTGCTGGCCTT
  GGGTGCGGCACTCGTGAATGTACAGATCCCCCTGCTCCTGGGCCAGCTGGTAGAGGTCGT
  GGCCAAGTACACAAGGGACCACGTAGGGAGTTTCATGACTGAGTCCCAGAATCTCAGCAC
  CCACCTGCTTATCCTCTATGGTGTCCAGGGACTGCTGACCTTCGGGTACCTGGTGCTGCT
  GTCCCACGTTGGCGAGCGCATGGCTGTGGACATGCGGAGGGCCCTCTTCAGCTCCCTGCT
  CCGACNAGACATCACCTTCTTTGACGCCAATAAGACAGGGCAGCTGGTGAGCCGCTTGAC
  AACTGACGTGCANGAGTTTTAAGTCATCCTTCAAGCTTGTCATCTCCCAGGGNCTGCNGA
  ACTGCACCCAGNT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007188 unedited
  CACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTGGTGGGAGGGTAGGCAGCCGG
  CAGGACTGGGGAGGGGAGGGAGGGATTGGCCTCCCCTGCCAGCTGCTCGGTCCCGGCTTT
  ATTGTGAGCAGGAGCAGTCGCATCGACTCTACACATGCTGAGTCCTCCTGGGGGCTCATT
  TCTTATTAGGCTCTTCCAGGCTGAGTCCCATCCCTAACACTGATGCTTGGTAGTGACCAT
  CCTTAGGGGGTCCTTTTCATGACTTGTGCTGCTGGTTACTGGGGTCTTCTGGTTTTTCGG
  CTCGGTGGGGTTCGATGTCCTTGGGGTATTCTGGGCCTGTCTCCGGATGATCCCGACGCA
  CATCCCGCCTCTCTTCAGGCAGCTCCTTTTGTTGCTTTATCTCTTTCACTCGGTCTTTGT
  CTTTGTAAACATTGATTTTGGTTTTTTTCGCTCCCTGCTTGTATTCTCTGGTTACTTCAT
  TCTTCACTCCATCGGTTCCGCTCTGCTACTGTATCTTGTTCTTTCGCTTATATTTCCCTT
  CTCTTTTCTCTTCTCCTCTCTGGTTTCTTCTTACTTACCATTCCTCCTCTCTCTCCTCCC
  ACTGGCCTCTTCTTCCGCTATTTTTCTCCCTCCTCCCTCCTCCTTTTGTTTCTCCCCTTC
  TCTTCTTTCTTCCCCACATCCTACTACTTCGCACCCATCTCTTCGTGTTTTTTTCAGTTC
  TTTCCTCCCCTCTTCTCTCTTTCCCCCCTCTCCTCCCTTTTCCTCTCTCTCTGTTTTCTT
  TTATTTCCTTTCTCATTTACTTACTGCTTCTCCTCACTTTCGATATCATTTACTTTTCTA
  CTCCCCCACTTTCTCTGTCTTATCTACTCTCCTTCCTTCCTACTTACTTGTTACCTCTTT
  ACTTCCCCTTTCTTCCCCTTCACCCCTTCTTCTTTCTCTCTCCTACATTCTCTCTCTCCT
  CTCACTGCTCCTTTTTTAACTCTCTCTCTCTTTTCCCCTCTTTTTTTTCTCTTCTTCTTT
  ATACCACGCCCCTCTATCNCCACTCACTCTCCTTCTACGCAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007188
• Insert Size: 2560 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007188.2, NP_009119.1
• RefSeq Size: 2439 bp
• RefSeq ORF: 2157 bp
• Locus ID: 11194
• UniProt ID: Q9NUT2
• Cytogenetics: 7q36.1
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: This nuclear gene encodes a multi-pass membrane protein that is targeted to the mitochondrial inner membrane. The encoded protein is an ATP-dependent transporter that may mediate the passage of organic and inorganic molecules out of the mitochondria. Loss of function of the related gene in mouse results in a disruption of iron homeostasis between the mitochondria and cytosol. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.