DDX58 (NM_014314) Human Untagged Clone

CAT#: SC115033

DDX58 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX58
• Synonyms: RIG-I; RIG1; RIGI; RLR-1; SGMRT2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014314 edited
  GAATTCGGCACGAGGAAAGTTCCTATGCAGCTCCGCCTCGCGTCCGGCCTCATTTCCTCG
  GAAAATCCCTGCTTTCCCCGCTCGCCACGCCCTCCTCCTACCCGGCTTTAAAGCTAGTGA
  GGCACAGCCTGCGGGGAACGTAGCTAGCTGCAAGCAGAGGCCGGCATGACCACCGAGCAG
  CGACGCAGCCTGCAAGCCTTCCAGGATTATATCCGGAAGACCCTGGACCCTACCTACATC
  CTGAGCTACATGGCCCCCTGGTTTAGGGAGGAAGAGGTGCAGTATATTCAGGCTGAGAAA
  AACAACAAGGGCCCAATGGAGGCTGCCACACTTTTTCTCAAGTTCCTGTTGGAGCTCCAG
  GAGGAAGGCTGGTTCCGTGGCTTTTTGGATGCCCTAGACCATGCAGGTTATTCTGGACTT
  TATGAAGCCATTGAAAGTTGGGATTTCAAAAAAATTGAAAAGTTGGAGGAGTATAGATTA
  CTTTTAAAACGTTTACAACCAGAATTTAAAACCAGAATTATCCCAACCGATATCATTTCT
  GATCTGTCTGAATGTTTAATTAATCAGGAATGTGAAGAAATTCTACAGATTTGCTCTACT
  AAGGGGATGATGGCAGGTGCAGAGAAATTGGTGGAATGCCTTCTCAGATCAGACAAGGAA
  AACTGGCCCAAAACTTTGAAACTTGCTTTGGAGAAAGAAAGGAACAAGTTCAGTGAACTG
  TGGATTGTAGAGAAAGGTATAAAAGATGTTGAAACAGAAGATCTTGAGGATAAGATGGAA
  ACTTCTGACATACAGATTTTCTACCAAGAAGATCCAGAATGCCAGAATCTTAGTGAGAAT
  TCATGTCCACCTTCAGAAGTGTCTGATACAAACTTGTACAGCCCATTTAAACCAAGAAAT
  TACCAATTAGAGCTTGCTTTGCCTGCTATGAAAGGAAAAAACACAATAATATGTGCTCCT
  ACAGGTTGTGGAAAAACCTTTGTTTCACTGCTTATATGTGAACATCATCTTAAAAAATTC
  CCACAAGGACAAAAGGGGAAAGTTGTCTTTTTTGCGAATCAGATCCCAGTGTATGAACAG
  CAGAAATCTGTATTCTCAAAATACTTTGAAAGACATGGGTATAGAGTTACAGGCATTTCT
  GGAGCAACAGCTGAGAATGTCCCAGTGGAACAGATTGTTGAGAACAATGACATCATCATT
  TTAACTCCACAGATTCTTGTGAACAACCTTAAAAAGGGAACGATTCCATCACTATCCATC
  TTTACTTTGATGATATTTGATGAATGCCACAACACTAGTAAACAACACCCGTACAATATG
  ATCATGTTTAATTATCTAGATCAGAAACTTGGAGGATCTTCAGGCCCACTGCCCCAGGTC
  ATTGGGCTGACTGCCTCGGTTGGTGTTGGGGATGCCAAAAACACAGATGAAGCCTTGGAT
  TATATCTGCAAGCTGTGTGCTTCTCTTGATGCGTCAGTGATAGCAACAGTCAAACACAAT
  CTGGAGGAACTGGAGCAAGTTGTTTATAAGCCCCAGAAGTTTTTCAGGAAAGTGGAATCA
  CGGATTAGCGACAAATTTAAATACATCATAGCTCAGCTGATGAGGGACACAGAGAGTCTG
  GCAAAGAGAATCTGCAAAGACCTCGAAAACTTATCTCAAATTCAAAATAGGGAATTTGGA
  ACACAGAAATATGAACAATGGATTGTTACAGTTCAGAAAGCATGCATGGTGTTCCAGATG
  CCAGACAAAGATGAAGAGAGCAGGATTTGTAAAGCCCTGTTTTTATACACTTCACATTTG
  CGGAAATATAATGATGCCCTCATTATCAGTGAGCATGCACGAATGAAAGATGCTCTGGAT
  TACTTGAAAGACTTCTTCAGCAATGTCCGAGCAGCAGGATTCGATGAGATTGAGCAAGAT
  CTTACTCAGAGATTTGAAGAAAAGCTGCAGGAACTAGAAAGTGTTTCCAGGGATCCCAGC
  AATGAGAATCCTAAACTTGAAGACCTCTGCTTCATCTTACAAGAAGAGTACCACTTAAAC
  CCAGAGACAATAACAATTCTCTTTGTGAAAACCAGAGCACTTGTGGACGCTTTAAAAAAT
  TGGATTGAAGGAAATCCTAAACTCAGTTTTCTAAAACCTGGCATATTGACTGGACGTGGC
  AAAACAAATCAGAACACAGGAATGACCCTCCCGGCACAGAAGTGTATATTGGATGCATTC
  AAAGCCAGTGGAGATCACAATATTCTGATTGCCACCTCAGTTGCTGATGAAGGCATTGAC
  ATTGCACAGTGCAATCTTGTCATCCTTTATGAGTATGTGGGCAATGTCATCAAAATGATC
  CAAACCAGAGGCAGAGGAAGAGCAAGAGGTAGCAAGTGCTTCCTTCTGACTAGTAATGCT
  GGTGTAATTGAAAAAGAACAAATAAACATGTACAAAGAAAAAATGATGAATGACTCTATT
  TTACGCCTTCAGACATGGGACGAAGCAGTATTTAGGGAAAAGATTCTGCACATACAGACT
  CATGAAAAATTCATCAGAGATAGTCAAGAAAAACCAAAACCTGTACCTGATAAGGAAAAT
  AAAAAACTGCTCTGCAGAAAGTGCAAAGCCTTGGCATGTTACACAGCTGACGTAAGAGTG
  ATAGAGGAATGCCATTACACTGTGCTTGGAGATGCTTTTAAGGAATGCTTTGTGAGTAGA
  CCACATCCCAAGCCAAAGCAGTTTTCAAGTTTTGAAAAAAGAGCAAAGATATTCTGTGCC
  CGACAGAACTGCAGCCATGACTGGGGAATCCATGTGAAGTACAAGACATTTGAGATTCCA
  GTTATAAAAATTGAAAGTTTTGTGGTGGAGGATATTGCAACTGGAGTTCAGACACTGTAC
  TCGAAGTGGAAGGACTTTCATTTTGAGAAGATACCATTTGATCCAGCAGAAATGTCCAAA
  TGATATCAGGTCCTCAATCTTCAGCTACAGGGAATGAGTAACTTTGAGTGGAGAAGAAAC
  AAACATAGTGGGTATAATCATGGATCGCTTGTACCCCTGTGAAAATATATTTTTTAAAAA
  TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014314 unedited
  ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGNAAAGTTCCTATGCAGCTCCGC
  CTCGCGTCCGGCCTCATTTCCTCGGAAAATCCCTGCTTTCCCCGCTCGCCACGCCCTCCT
  CCTACCCGGCTTTAAAGCTAGTGAGGCACAGCCTGCGGGGAACGTAGCTAGCTGCAAGCA
  GAGGCCGGCATGACCACCGAGCAGCGACGCAGCCTGCAAGCCTTCCAGGATTATATCCGG
  AAGACCCTGGACCCTACCTACATCCTGAGCTACATGGCCCCCTGGTTTAGGGAGGAAGAG
  GTGCAGTATATTCAGGCTGAGAAAAACAACAAGGGCCCAATGGAGGCTGCCACACTTTTT
  CTCAAGTTCCTGTTGGAGCTCCAGGAGGAAGGCTGGTTCCGTGGCTTTTTGGATGCCCTA
  GACCATGCAGGTTATTCTGGACTTTATGAAGCCATTGAAAGTTGGGATTTCAAAAAAATT
  GAAAAGTTGGAGGAGTATAGATTACTTTTAAAACGTTTACAACCAGAATTTAAAACCAGA
  ATTATCCCAACCGATATCATTTCTGATCTGTCTGAATGTTTAATTAATCAGGAATGTGAA
  GAAATTCTACAGATTTGCTCTACTAAGGGGATGATGGCAGGTGCAGAGAAATTGGTGGAA
  TGCCTTCTCAGATCAGACAAGGAANACTGGCCCAAAACTTTGAAACTTGCTTTGGAGAAA
  GAAGGAAACAGTTCAGTGAACTGTGGATTGTAGAGAAAGTATCAAAGATGTTGAAACAGA
  AGATCTTGCAGACTAGATGGGAACTTCTGACATACAGATTTTCTACCAAGAAGATTCCAG
  AATGCCGAATCCTTAGTGAGCATTCATGTTCACCCTTCAGAAGTGTCTGATCCAAACTGC
  ACAGCCCATTNAACCAGAAATACATTTACAGCCTGCCTTGCTGCTATGAAGGNAACACAC
  ATATATGGCTCCTACGGGNTGGGGAAAAACCTTGTTCACTGTTACTCGGACTCATCTAAA
  ATCCAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014314 unedited
  ACTATGAACCCGCGGCCGCAATCTAGNGATCAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTATTTTTAAAAAAATATTTTCACAGGGGTACAAGCGATCCATGATTATACC
  CACTATGTTTGTTTCTTCTCCACTCAAAGTTACTCATTCCCTGTAGCTGAAGATTGAGGA
  CCTGATATCATTTGGACATTTCTGCTGGATCAAATGGTATCTTCTCAAAATGAAAGTCCT
  TCCACTTCGAGTACAGTGTCTGAACTCCAGTTGCAATATCCTCCACCACAAAACTTTCAA
  TTTTTATAACTGGAATCTCAAATGTCTTGTACTTCACATGGATTCCCCAGTCATGGCTGC
  AGTTCTGTCGGGCACAGAATATCTTTGCTCTTTTTTCAAAACTTGAAAACTGCTTTGGCT
  TGGGATGTGGTCTACTCACAAAGCATTCCTTAAAAGCATCTCCAAGCACAGTGTAATGGC
  ATTCCTCTATCACTCTTACGTCAGCTGTGTAACATGCCAAGGCTTTGCACTTTCTGCAGA
  GCAGTTTTTTATTTTCCTTATCAGGTACAGGTTTTGGTTTTTCTTGACTATCTCTGATGA
  ATTTTTCATGAGTCTGTATGTGCAGAATCTTTTCCCTAAATACTGCTTCGTCCCATGTCT
  GAAGGCGTAAAATAGAGTCATTCATCATTTTTTCTTTGNACATGTTTATTTGTTCTTTTT
  CAATTACACCAGCATTACTAGTCAGAAGGAAGCACTTGCTACCTCTTGCTCTTCCTCTGC
  CTCTGGTTTGGATTCATTTGATGACATTGCCCACATACTCATAAAGGATGACAAGATTGC
  ACTGTGCAATGTCAATGCCTTCATCAGCAACTGGAGGTGGCATCAGAATATTTGGATCTC
  CACTGGGCTTGAATGCATCCATATACCTTCTGTGCCCGGAGGGNCNTNCCTGGGTTCTGA
  ATTGTTTTGCCCGTCCAGTCAATATGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014314
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014314.2, NP_055129.2
• RefSeq Size: 4372 bp
• RefSeq ORF: 2778 bp
• Locus ID: 23586
• UniProt ID: O95786
• Cytogenetics: 9p21.1
• Domains: DEAD, helicase_C
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2. [provided by RefSeq, Aug 2020]