HPGDS (NM_014485) Human Untagged Clone

CAT#: SC114983

HPGDS (untagged)-Human hematopoietic prostaglandin D synthase (HPGDS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HPGDS
• Synonyms: GSTS; GSTS1; GSTS1-1; PGD2; PGDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114983 sequence for NM_014485 edited (data generated by NextGen Sequencing)
  ATGCCAAACTACAAACTCACTTATTTTAATATGAGGGGGAGAGCAGAAATTATTCGTTAC
  ATATTTGCTTATTTGGACATACAGTATGAAGACCACAGAATAGAACAAGCTGACTGGCCT
  GAAATCAAATCAACTCTCCCATTTGGAAAAATCCCCATTTTGGAAGTTGATGGACTTACT
  CTTCACCAGAGCCTAGCAATAGCAAGATATTTGACCAAAAACACAGATTTGGCTGGAAAC
  ACAGAAATGGAACAATGTCATGTTGATGCTATTGTGGACACTCTGGATGATTTCATGTCA
  TGTTTTCCTTGGGCAGAGAAAAAGCAAGATGTGAAAGAGCAGATGTTCAATGAGCTGCTC
  ACGTATAATGCGCCTCATCTTATGCAAGACTTGGACACATATTTAGGGGGGAGAGAATGG
  CTTATTGGTAACTCTGTAACTTGGGCAGACTTCTACTGGGAGATTTGCAGTACCACACTT
  TTGGTCTTTAAGCCTGACCTGTTAGACAACCATCCAAGGCTGGTGACTTTACGGAAGAAA
  GTCCAAGCCATTCCTGCCGTCGCTAACTGGATAAAACGAAGGCCCCAAACCAAACTCTAG
  
  Clone variation with respect to NM_014485.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014485 unedited
  TGCGGATTTGTATACGACTCATATAGGGCGGCCGCGAATCGGCACGAGGACTTACTCGTC
  ATATAAACCCAAGGCACAGTCACATACCCAGGGATACAAGACACTGCAGACTCCCGAGAG
  ACATAACACAGAATTGCACCATGCCAAACTACAAACTCACTTATTTTAATATGAGGGGGA
  GAGCAGAAATTATTCGTTACATATTTGCTTATTTGGACATACAGTATGAAGACCACAGAA
  TAGAACAAGCTGACTGGCCTGAAATCAAATCAACTCTCCCATTTGGAAAAATCCCCATTT
  TGGAAGTTGATGGACTTACTCTTCACCAGAGCCTAGCAATAGCAAGATATTTGACCAAAA
  ACACAGATTTGGCTGGAAACACAGAAATGGAACAATGTCATGTTGATGCTATTGTGGACA
  CTCTGGATGATTTCATGTCATGTTTTCCTTGGGCAGAGAAAAAGCAAGATGTGAAAGAGC
  AGATGTTCAATGAGCTGCTCACGTATAATGCGCCTCATCTTATGCAAGACTTGGACACAT
  ATTTAGGGGGGAGAGAATGGCTTATTGGTAACTCTGTAACTTGGGCAGACTTCTACTGGG
  AGATTTGCAGTACCACACTTTTGGTCTTTAAGCCTGACCTGTTAGACAACCATCCAAGGC
  TGGTGACTTTACGGAAGNAAGTCCAAGCCATTCCTGCCGTCGCTAACTGGATAAAACGAA
  GGCCCCAAACCAAACTCTAGCTGATCCATGTTGCCCTCAAGTTTTGTTTTCTTCGGGGCA
  TCTCTCTTATCAGATAGACAGCTACATCAGCCTGCCAGATATCCACATGCTCCCTCCCCA
  GCGTCACTAAGATTTTCACTTTTACCATATTCTGATTTTTAAAAGGAAAAAAAAACAAAT
  CTTTTTTAGTAAAAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014485
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014485.2, NP_055300.1
• RefSeq Size: 1615 bp
• RefSeq ORF: 600 bp
• Locus ID: 27306
• UniProt ID: O60760
• Cytogenetics: 4q22.3
• Domains: GST_N, GST_C
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: Prostaglandin-D synthase is a sigma class glutathione-S-transferase family member. The enzyme catalyzes the conversion of PGH2 to PGD2 and plays a role in the production of prostanoids in the immune system and mast cells. The presence of this enzyme can be used to identify the differentiation stage of human megakaryocytes. [provided by RefSeq, Jul 2008]