Argininosuccinate Lyase (ASL) (NM_000048) Human Untagged Clone

CAT#: SC114664

ASL (untagged)-Human argininosuccinate lyase (ASL), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASL
• Synonyms: ASAL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000048, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCTCGGAGAGTGGGAAGCTTTGGGGTGGCCGGTTTGTGGGTGCAGTGGACCCCATCATGGAGAAGT
  TCAACGCGTCCATTGCCTACGACCGGCACCTTTGGGAGGTGGATGTTCAAGGCAGCAAAGCCTACAGCAG
  GGGCCTGGAGAAGGCAGGGCTCCTCACCAAGGCCGAGATGGACCAGATACTCCATGGCCTAGACAAGGTG
  GCTGAGGAGTGGGCCCAGGGCACCTTCAAACTGAACTCCAATGATGAGGACATCCACACAGCCAATGAGC
  GCCGCCTGAAGGAGCTCATTGGTGCAACGGCAGGGAAGCTGCACACGGGACGGAGCCGGAATGACCAGGT
  GGTCACAGACCTCAGGCTGTGGATGCGGCAGACCTGCTCCACGCTCTCGGGCCTCCTCTGGGAGCTCATT
  AGGACCATGGTGGATCGGGCAGAGGCGGAACGTGATGTTCTCTTCCCGGGGTACACCCATTTGCAGAGGG
  CCCAGCCCATCCGCTGGAGCCACTGGATTCTGAGCCACGCCGTGGCACTGACCCGAGACTCTGAGCGGCT
  GCTGGAGGTGCGGAAGCGGATCAATGTCCTGCCCCTGGGGAGTGGGGCCATTGCAGGCAATCCCCTGGGT
  GTGGACCGAGAGCTGCTCCGAGCAGAACTCAACTTTGGGGCCATCACTCTCAACAGCATGGATGCCACTA
  GTGAGCGGGACTTTGTGGCCGAGTTCCTGTTCTGGGCTTCGCTGTGCATGACCCATCTCAGCAGGATGGC
  CGAGGACCTCATCCTCTACTGCACCAAGGAATTCAGCTTCGTGCAGCTCTCAGATGCCTACAGCACGGGA
  AGCAGCCTGATGCCCCAGAAGAAAAACCCCGACAGTTTGGAGCTGATCCGGAGCAAGGCTGGGCGTGTGT
  TTGGGCGGTGTGCCGGGCTCCTGATGACCCTCAAGGGACTTCCCAGCACCTACAACAAAGACTTACAGGA
  GGACAAGGAAGCTGTGTTTGAAGTGTCAGACACTATGAGTGCCGTGCTCCAGGTGGCCACTGGCGTCATC
  TCTACGCTGCAGATTCACCAAGAGAACATGGGACAGGCTCTCAGCCCCGACATGCTGGCCACTGACCTTG
  CCTATTACCTGGTCCGCAAAGGGATGCCATTCCGCCAGGCCCACGAGGCCTCCGGGAAAGCTGTGTTCAT
  GGCCGAGACCAAGGGGGTCGCCCTCAACCAGCTGTCACTGCAGGAGCTGCAGACCATCAGCCCCCTGTTC
  TCGGGCGACGTGATCTGCGTGTGGGACTACGGGCACAGTGTGGAGCAGTATGGTGCCCTGGGCGGCACTG
  CGCGCTCCAGCGTCGACTGGCAGATCCGCCAGGTGCGGGCGCTACTGCAGGCACAGCAGGCCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000048 unedited
  GTGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGANAGAGAGAGA
  GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
  GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCGGAGGTGAGTGCGCGGCGG
  CCGGATGGGCGGGACGGGCGTGGAGGACGCCGAGCACCGTGGCGCGCGCTCACGTCCGCG
  TCCCCAAGGGCTGCGCTCCCTCAAGCGCAGTGCCCAGAACTCGGAGCCAGCCCGGCCCGG
  GGGACCCTGCTGGCCAAGGAGGTCGTCAGTCCGGTCTTGTCTTCCAGACCCGGAGGACCG
  AAGCTTCCGGACGACGAGGAACCGCCCAACATGGCCTCTGAGAGTGGGAAGCTTTGGGGT
  GGCCGGTTTGTGGGTGCAGTGGACCCCATCATGGAGAAGTTCAACGCGTCCATTGCCTAC
  GACCGGCACCTTTGGGAGGTGGATGTTCAAGGCAGCAAAGCCTACAGCTGGGGCCTGGAG
  AAGGCAGGGCTCCTCACCAAGGCCGAGATGGACCAGATACTCCATGGCCTAGACAAGGTG
  GCTGAGGAGTGGGCCCAGGGCACCTTCAAACTGAACTCCAATGATGAGGACATCCACACA
  GCCAATGAGCGCCGCCTGAAGGAGCTCATTGGTGCAACGGCAGGGAAGCTGCTCACGGGA
  CGGAGCCGNGATGACCAGGTGGTCACAGACCTCAGGCTGTGGATGCGGCAGACCTGCTTC
  ACGCTCTCGGGCCTCCTCTGGGAGCTCATTATGACCATGGTGGATCGCGCAGATGCTGGA
  CGTGATGTTCTCTTCCCGCGTACACCCATTTGCAGAGGGCCCAGCCCATCCGCTGGAGCC
  ACTGGATTCTGAGCCACGCCGTGCACTTGACCGAGACTCTGAGCGGTTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000048 unedited
  GCGGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTCCTCTCGCCCCCACTTTTATTAGGGGGCAGGTGTGGAAGGACCTAGGCCT
  GTTGTGCCTGCAATACCCCCCGCACCTGGGGGATCTGCCATTCAACCCTGGAGCGCCCAG
  TGCCCCCCAGGGCACCATACTGTTCCACACTGGGCCCGTAGTCCCACACCCAAATCACGT
  CCCCCGAAAACAGGGGGCTGATGGTCTGCAACTCCTGCAGTGACAGTTGGTTGAGGGCGA
  CCCCCTTGGTCTGGGCCATGAACACAGTTTTCCCGGAGGCCTTGGGGGCCTGGCGGAATG
  GCATCCCTTTGCGGACCAGGTAATAGGCAAGGTCAGGGGCCACCATGTCGGGGCTGAAAC
  CCTGTCCCATGTTCTCTTGGGGAATCTGCAGCGTAAAGATGACCCCAGTGGCCACCTGGA
  GCACGGCACTCATAGGGTCTGACACTTAAAACACAGTTTCCTTGGCCTCCTGTAAGTCTT
  TGTTGGAGGCGCTGGGAAATCCCTTGAGGGTCATCAGGAGCCCGGAACACCGCCCAAAAC
  ACCCCCCACCTTGCTCGGAATAGCTCCAAACTGTTGGGGTTTTTTTTTTGGGGCATAAGG
  CTGTTTCCCGTGCTGGAGCATTTGAAGCTGCCCAAACCTGAATCCTTGGGGCAATAAAGA
  AAAGGCCCCCGGCCTCCTGTTGAAAGGGTATGCCCCGGAAAACCAAACAGGACCTGGCCC
  AAAATCCCGTTACTATGGCATTCTGGCGTTGAAAGGATGGCCCAAATTGAATTTTGTTTG
  AACAACTTTGGGCCAACCAAGGGAAGGCCGGAAGGGCCCCTTCCCCAGGGAGGAAATTTA
  TCCTTTCCCACCTTAAAGCGCTAAAATTGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000048
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000048.3, NP_000039.2
• RefSeq Size: 1937 bp
• RefSeq ORF: 1395 bp
• Locus ID: 435
• UniProt ID: P04424
• Cytogenetics: 7q11.21
• Domains: lyase_1
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1).