HSD17B12 (NM_016142) Human Untagged Clone

CAT#: SC114479

HSD17B12 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 12 (HSD17B12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSD17B12
• Synonyms: KAR; SDR12C1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114479 sequence for NM_016142 edited (data generated by NextGen Sequencing)
  ATGGAGAGCGCTCTCCCCGCCGCCGGCTTCCTGTACTGGGTCGGCGCGGGCACCGTGGCC
  TACCTAGCCCTGCGTATTTCGTACTCGCTCTTCACGGCCCTCCGGGTCTGGGGAGTGGGG
  AATGAGGCGGGGGTCGGCCCGGGGCTCGGAGAATGGGCAGTTGTCACAGGTAGTACTGAT
  GGAATTGGAAAATCATATGCAGAAGAGTTAGCAAAGCATGGAATGAAGGTTGTCCTTATC
  AGCAGATCAAAGGATAAACTTGACCAGGTTTCCAGTGAAATAAAAGAAAAATTCAAAGTG
  GAGACAAGAACCATTGCTGTTGACTTTGCATCAGAAGATATTTATGATAAAATTAAAACA
  GGCTTGGCTGGTCTTGAAATCGGCATCTTAGTGAACAACGTGGGAATGTCGTATGAGTAT
  CCTGAATACTTTTTGGATGTTCCTGACTTGGACAATGTGATCAAGAAAATGATAAATATT
  AATATTCTTTCTGTTTGTAAGATGACACAATTGGTACTGCCTGGCATGGTGGAAAGATCC
  AAAGGGGCTATTCTGAACATTTCATCTGGCAGTGGCATGCTCCCTGTCCCACTCTTGACC
  ATCTATTCTGCAACCAAGACTTTTGTAGATTTCTTCTCTCAGTGCCTCCATGAGGAGTAT
  AGGAGCAAGGGCGTCTTTGTGCAGAGTGTCCTGCCATACTTCGTAGCTACAAAACTGGCT
  AAAATCCGGAAGCCAACTTTGGATAAGCCCTCTCCGGAGACGTTTGTGAAGTCTGCAATT
  AAAACAGTCGGCCTGCAATCCCGAACCAATGGATACCTGATCCATGCTCTTATGGGCTCG
  ATAATCTCAAACCTGCCTTCTTGGATTTATTTGAAAATAGTCATGAATATGAACAAGTCT
  ACACGGGCTCACTATCTGAAGAAAACCAAGAAGAACTAA
  
  Clone variation with respect to NM_016142.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016142 unedited
  NGGTAACGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGGCACGAGGG
  GAATGGCCCCGCGGGCGGGGTTTAGGCCCAAATGGTGTCGGAGCAGCGCCTATTAGTGTC
  ATCCTCACCGTCACGGCCGGCGCCTCCTCCTGGATTCATTCACTCGCTCTTTTCATTCAC
  GAAGGTAGTGAGGCCTAGTGGAAAGCCATGGAGAGCGCTCTCCCCGCCGCCGGCTTCCTG
  TACTGGGTCGGCGCGGGCACCGTGGCCTACCTAGCCCTGCGTATTTCGTACTCGCTCTTC
  ACGGCCCTCCGGGTCTGGGGAGTGGGGAATGAGGCGGGGGTCGGCCCGGGGCTCGGAGAA
  TGGGCAGTTGTCACAGGTAGTACTGATGGAATTGGAAAATCATATGCAGAAGAGTTAGCA
  AAGCATGGAATGAAGGTTGTCCTTATCAGCAGATCAAAGGATAAACTTGACCAGGTTTCC
  AGTGAAATAAAAGAAAAATTCAAAGTGGAGACAAGAACCATTGCTGTTGACTTTGCATCA
  GAAGATATTTATGATAAAATTAANACAGGCTTGGCTGGTCTTGAAATCGGCATCTTAGTG
  AACAACGTGNGAATGTCGTATGAGTATCCTGAATACTTTTTGGATGTTCCTGACTTGGAC
  AATGTGATCAAGGAAATGATAAATATTAATATTCTTTCTGTTTGTAAGATGACACAATTG
  GTACTGCCTGGCATGGTGGAAAGATCCCAAGGGGCTATTCTGAACATTTCATCTGGCAGT
  GGCATGCGTCCTGTCCCACTCTTGACCATCTATTCTGCAACCAAAGACTTTTGTAAATTT
  CTTCTCTCAGTGCCTTCCATGAGGAGTATAGNACCAAGGGGCGTCTTTTGTGCCGAATGT
  CCCTGCCTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016142 unedited
  GCCCATTACTATGNACCGCGGCACGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTCA
  TAAAGCTATATATTTATTCAACTTAAATGACAACCTTTATTCTGAGATTACATTCTTCCT
  AATTTTAGGGGCTAAAATATATTTTCTTTTACATAATGATGCTGATAGCAGATGGCTATT
  TGTTTTTGTTTGTTTATTTTTGTTTTATGTCCTTAGCTGGCAAAATTAAAAGCCAGAAGC
  TAAGGCCCCATTTTTTTTTCTTTTAATACAAATCTACTGGTGCTGAAAACTCAAAGCTTA
  GGAAACACAGCCTAGGTAAAGACCAATCTTCTTGCTGCATATTTCACAGTATTGAATTCT
  TTCTTGGCGAGTTCTCCATACAAGTTATGAAGCAGGATAAAAGTCACTCTTATATTAAGC
  CATTGTAAATACGGCCTTAATTTCACTCACCACACAACTGTATTTTTGTTGTACACCATG
  GTCGCACCATGTTTTATTAAACCTAACTCTATCACTAGCTACCTGCACAGTATCAACTTA
  CAAATTATACTGCCATTTCTATGAAGCCATCACTTTTATAGGACTACCCCGTGCCATATG
  TGTGACCAATATTGGCTTAATGCATAAACAAAGGCAGCTTGTGTGTCCAAGCAAGCCATT
  TTTCTGGGCCACGGTCTTAGTGCCACCCTATATGGCTGCCAGTCTCCATGTACACTCTGG
  CCCATTTATTTTTTATAACCATCCCATATAGCCCCGACTTCCCCCTACCTTTACACTGAA
  CACAACCTTCCTCTATAGAACGCTTACTCTAACGGCGCTCCATGGGCCACAAATACTTTC
  ATATTTACACTTATTTCCTTGCCTCTCACTCCCTTCGAAAACAACATTCCCTCATACCGC
  CCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016142
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016142.1, NP_057226.1
• RefSeq Size: 2393 bp
• RefSeq ORF: 939 bp
• Locus ID: 51144
• UniProt ID: Q53GQ0
• Cytogenetics: 11p11.2
• Domains: adh_short
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Biosynthesis of unsaturated fatty acids, Metabolic pathways
• Gene Summary: This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. [provided by RefSeq, Oct 2011]