LEF1 (NM_016269) Human Untagged Clone

CAT#: SC114370

LEF1 (untagged)-Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LEF1
• Synonyms: LEF-1; TCF1ALPHA; TCF7L3; TCF10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016269 edited
  GAATTCGGCACCAGGCTGCACGAACCCTTCCAACTCTCCTTTCCTCCCCCACCCTTGAGT
  TACCCCTCTGTCTTTCCTGCTGTTGCGCGGGTGCTCCCACAGCGGAGCGGAGATTACAGA
  GCCGCCGGGATGCCCCAACTCTCCGGAGGAGGTGGCGGCGGCGGGGGGGACCCGGAACTC
  TGCGCCACGGACGAGATGATCCCCTTCAAGGACGAGGGCGATCCTCAGAAGGAAAAGATC
  TTCGCCGAGATCAGTCATCCCGAAGAGGAAGGCGATTTAGCTGACATCAAGTCTTCCTTG
  GTGAACGAGTCTGAAATCATCCCGGCCAGCAACGGACACGAGGTGGCCAGACAAGCACAA
  ACCTCTCAGGAGCCCTACCACGACAAGGCCAGAGAACACCCCGATGACGGAAAGCATCCA
  GATGGAGGCCTCTACAACAAGGGACCCTCCTACTCGAGTTATTCCGGGTACATAATGATG
  CCAAATATGAATAACGACCCATACATGTCAAATGGATCTCTTTCTCCACCCATCCCGAGA
  ACATCAAATAAAGTGCCCGTGGTGCAGCCATCCCATGCGGTCCATCCTCTCACCCCCCTC
  ATCACTTACAGTGACGAGCACTTTTCTCCAGGATCACACCCGTCACACATCCCATCAGAT
  GTCAACTCCAAACAAGGCATGTCCAGACATCCTCCAGCTCCTGATATCCCTACTTTTTAT
  CCCTTGTCTCCGGGTGGTGTTGGACAGATCACCCCACCTCTTGGCTGGCAAGGTCAGCCT
  GTATATCCCATCACGGGTGGATTCAGGCAACCCTACCCATCCTCACTGTCAGTCGACACT
  TCCATGTCCAGGTTTTCCCATCATATGATTCCCGGTCCTCCTGGTCCCCACACAACTGGC
  ATCCCTCATCCAGCTATTGTAACACCTCAGGTCAAACAGGAACATCCCCACACTGACAGT
  GACCTAATGCACGTGAAGCCTCAGCATGAATAGAGAAAGGAGCAGGAGCCAAAAAGACCT
  CACATTAAGAAGCCTCTGAATGCTTTTATGTTATACATGAAAGAAATGAGAGCGAATGTC
  GTTGCTGAGTGTACTCTAAAAGAAAGTGCAGCTATCAACCAGATTCTTGGCAGAAGGTGG
  CATGCCCTCTCCCGTGAAGAGCAGGCTAAATATTATGAATTAGCACGGAAAGAAAGACAG
  CTACATATGCAGCTTTATCCAGGCTGGTCTGCAAGAGACAATTATGGTAAGAAAAAGAAG
  AGGAAGAGAGAGAAACTACAGGAATCTGCATCAGGTACAGGTCCAAGAATGACAGCTGCC
  TACATCTGAAACATGGTGGAAAACGAAGCTCATTCCCAATGTGCAAAGCCAAGGCAGCGA
  CCCCAGGACCTCTTCTGGAGATGGAAGCTTGTTGAAAACCCAGACTGTCTCCACGGCCTG
  CCCAGTCGACCCCAAAGGAACACTGACATCAATTTTACCCTGAGGTCACTGCTAGAGACG
  CTGATCCATAAAGACAATCACTGCCAACCCCTCTTTCGTCTACTGCAAGAGCCAAGTTCC
  AAAATAAAGCATAAAAAGGTTTTTTAAAAGGAAATGTAAAAGCACATGAGAATGCTAGCA
  GGCTGTGGGGCAGCTGAGCAGCTTTTCTCCCCCCATATCTGCGTGCACTTCCCAGAGCAT
  CTTGCATCCAAACCTGTAACCTTTCGGCAAGGACGGTAACTTGGCTGCATTTGCCTGTCA
  TGCGCAACTGGAGCCAGCAACCAGCACATCCATCAGCACCCCAGTGGAGGAGTTCATGGA
  AGAGTTCCCTCTTTGTTTCTGCTTCATTTTTCTTTCTTTTCTTTTCTCCTAAAGCTTTTA
  TTTAACAGTGCAAAAGGATCGTTTTTTTTTGCTTTTTTAAACTTGAATTTTTTTAATTTA
  CACTTTTTAGTTTTAATTTTCTTGTATATTTTGCTAGCTATGAGCTTTTAAATAAAATTG
  AAAGTTCTGGAAAAGTTTGAAATAATGACATAAAAAGAAGCCTTCTTTTTCTGAGACAGC
  TTGTCTGGTAAGTGGCTTCTCTGTGAATTGCCTGTAACACATAGTGGCTTCTCCGCCCTT
  GTAAGGTGTTCAGTAGAGCTAAATAAATGTAATAGCCAAACCCACTCTGTTGGTAGCAAT
  TGGCAGCCCTATTTCAGTTTATTTTTTCTTCTGTTTTCTTCTTTTCTTTTTTTAAACAGT
  AAACCTTAACAGATGCGTTCAGCAGACTGGTTTGCAGTGAATTTTCATTTCTTTCCTTAT
  CACCCCCTTGTTGTAAAAAGCCCAGCACTTGAATTGTTATTACTTTAAATGTTCTGTATT
  TGTATCTGTTTTTATTAGCCAATTAGTGGGATTTTATGCCAGTTGTTAAAATGAGCATTG
  ATGTACCCATTTTTTAAAAAAGCAAGGCACAGCCTTTGCCCAAAACTGTCATCCTAACGT
  TTGTCATTCCAGTTTGAGTTAATGTGCTGAGCATTTTTTTAAAAGAAGCTTTGTAATAAA
  ACATTTTTAAAAATTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016269 unedited
  NGTAACGTCAAATATTTGTATACGACTCACTNATAGGGCGGCCGCGAATTCGCACCAGGC
  TGCACGAACCCTTCCAACTCTCCTTTCCTCCCCCACCCTTGAGTTACCCCTCTGTCTTTC
  CTGCTGTTGCGCGGGTGCTCCCACAGCGGAGCGGAGATTACAGAGCCGCCGGGATGCCCC
  AACTCTCCGGAGGAGGTGGCGGCGGCGGGGGGGACCCGGAACTCTGCGCCACGGACGAGA
  TGATCCCCTTCAAGGACGAGGGCGATCCTCAGAAGGAAAAGATCTTCGCCGAGATCAGTC
  ATCCCGAAGAGGAAGGCGATTTAGCTGACATCAAGTCTTCCTTGGTGAACGAGTCTGAAA
  TCATCCCGGCCAGCAACGGACACGAGGTGGCCAGACAAGCACAAACCTCTCAGGAGCCCT
  ACCACGACAAGGCCAGAGAACACCCCGATGACGGAAAGCATCCAGATGGAGGCCTCTACA
  ACAAGGGACCCTCCTACTCGAGTTATTCCGGGTACATAATGATGCCAAATATGAATAACG
  ACCCATACATGTCAAATGGATCTCTTTCTCCACCCATCCCGAGAACATCAAATAAAGTGC
  CCGTGGTGCAGCCATCCCATGCGGTCCATCCTCTCACCCCCCTCATCACTTACAGTGACG
  AGCACTTTTCTCCAGGATCACACCCGTCACACATCCCATCAGATGTCAACTCCAAACAAG
  GCATGTCCAGACATCCTCCAGCTCCTGATATCCCTACTTTTTATCCCTTGTCTCCGGGTG
  GTGTTGGACAGATCACCCCACCTCTTGGCTGGCAAGGTCAGCCTGTATATCCCATCACGG
  GGTGGATTCAGGCAACCCTACCCATCCTCACTGTCAGTCGACACTTCCATGTTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016269 unedited
  TGTACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTAATTTTTAAAAAT
  GTTTTATTACAAAGCTTCTTTTAAAAAATGCTCAGCACATTAACTCAAACTGGAATGACA
  AACGTTAGGATGACAGTTTTGGGCAAAGGCTGTGCCTTGCTTTTTTAAAAAATGGGTACA
  TCAATGCTCATTTTAACAACTGGCATAAAATCCCACTAATTGGCTAATAAAAACAGATAC
  AAATACAGAACATTTAAAGTAATAACAATTCAAGTGCTGGGCTTTTTACAACAAGGGGGT
  GATAAGGAAAGAAATGAAAATTCACTGCAAACCAGTCTGCTGAACGCATCTGTTAAGGTT
  TACTGTTTAAAAAAAGAAAAGAAGAAAACAGAAGAAAAAATAAACTGAAATAGGGCTGCC
  AATTGCTACCAACAGAGTGGGTTTGGCTATTACATTTATTTAGCTCTACTGAACACCTTA
  CAAGGGCGGAGAAGCCACTATGTGTTACAGGCAATTCACAGAGAAGCCACTTACCAGACA
  AGCTGTCTCAGAAAAAGAAAGCTTCTTTTTATGTCATTATTTCAAACTTTTCCAGAACTT
  TCAATTTTATTTAAAAGCTCATAGCTAGCAAAATATACAAGATAATTAAAACTAAAAAGT
  GTAAATTAAAAAAATTCAAGCTTAAAAAAGCAAAAAAAAACGATCCTTTTGCACTGCTAA
  ATAAAAGCTTTACGAGAAAAGATAAGAAAGATAAATGAAGCCGAAACCAAGAAGGAACTC
  TTCCATGAACTCCTNCACTGGAGTGCTGATGGATGTGCTAGGTGCTGGGCTCCATTGCGC
  ATGACAGGCAATGCAGCCAAGTTACCGTCCTTGCCGAAGGTTACAAGTTTGGATGCANAA
  TGCTCCTGGAAGTGCACCCATTATGGGGGGAGATAGCTGCTCANCTGCCCACAGCCTGCT
  GCATTCTCATGGGCTTTAATTNNTCTTTAAAAAACCTTTTATGCTNAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016269
• Insert Size: 2660 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016269.2, NP_057353.1
• RefSeq Size: 3084 bp
• RefSeq ORF: 1200 bp
• Locus ID: 51176
• UniProt ID: Q9UJU2
• Cytogenetics: 4q25
• Domains: HMG
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway
• Gene Summary: This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).