POLR3K (NM_016310) Human Untagged Clone

CAT#: SC114310

POLR3K (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR3K
• Synonyms: C11; C11-RNP3; HLD21; My010; RPC10; RPC11; RPC12.5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016310 edited
  ATGCTGCTGTTCTGCCCCGGCTGCGGGAACGGGCTGATCGTGGAGGAGGGACAACGCTGC
  CACCGCTTCGCCTGCAACACGTGCCCCTACGTGCACAACATCACCCGCAAGGTAACAAAT
  CGGAAGTACCCAAAACTGAAAGAAGTGGATGATGTGCTTGGTGGAGCAGCTGCCTGGGAG
  AATGTTGACTCTACTGCAGAGTCGTGTCCCAAATGCGAACATCCTCGTGCTTACTTCATG
  CAGCTTCAGACCCGCTCTGCAGATGAGCCGATGACCACCTTCTACAAGTGCTGCAATGCT
  CAGTGTGGACACCGCTGGAGGGATTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016310 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCCGGGAGTTGGAGCCT
  GCGGAGTTCGAGACCATGCTGCTGTTCTGCCCCGGCTGCGGGAACGGGCTGATCGTGGAG
  GAGGGACAACGCTGCCACCGCTTCGCCTGCAACACGTGCCCCTACGTGCACAACATCACC
  CGCAAGGTAACAAATCGGAAGTACCCAAAACTGAAAGAAGTGGATGATGTGCTTGGTGGA
  GCAGCTGCCTGGGAGAATGTTGACTCTACTGCAGAGTCGTGTCCCAAATGCGAACATCCT
  CGTGCTTACTTCATGCAGCTTCAGACCCGCTCTGCAGATGAGCCGATGACCACCTTCTAC
  AAGTGCTGCAATGCTCAGTGTGGACACCGCTGGAGGGATTAGGGCCAGGATGGCCCAGCT
  GCCCTAGTGTGTGCTTGCCTTGTCCCTCGGGGTAGATGCTTAGCTGGCAGTATGAGTCGT
  GTGTCCTGAGGGTCTTTGCTAGTGTGGTGGAAAGATAAACCTTTTGAGGTGAAGAGCCAG
  GGGGTCAGGAAATATGGCCTATCTGCCAGGCAGGGTGGATGAAGTCATGAATGTCTGGGA
  GTTTTTCTGTGTGGGGAGGAGACAGAGACCCATAACTAAATATGCTCTGTGTAAAGTCCT
  ATTCTTTCATCTTCCACTNTATTGGCAGTTGACATTCCCTTACTCCCAATCAACACTCTT
  AAATATTTGTACTGTTTGTAAAACTTAGTACATGTCCCCTAATATTTANNANNANNANNN
  NNNNNNANANNAAAACTCGACTCTAGATTGCGCCGCGTCATAGCTGTTCCTGACAGATCC
  GGNTGCATCCTGTGACCCTNCCAGTGCTCTCTGGCCTGAAGTGCACTCAGTGCACCANCT
  GNCTATAAAATAGTGCATATTGCTGACTAGGCTATATTTGAGGGGGGGGGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016310 unedited
  GTCCGAGGCCGCTTTCTANATCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA
  TATTTAGGGACATGTACTAAGTTTTACAAACAGTACAAATATTTAAAAGTGTTGATTGGG
  AGTAAGGGAATGTCAACTGCCAATAAAGGGGAAGATGAAAGAATAGGACTTTACACAGAG
  CATATTTAGTTATGGGTCTCTGTCTCCTCCCCACACAGAAAAACTCCCAGACATTCATGA
  CTTCATCCACCCTGCCTGGCAAATAGGCCATATTTCCTGACCCCCTGGCTCTTCACCTCA
  AAAGGTTTATCTTTCCACCACACTAGCAAAGACCCTCAGGACACACGACTCATACTGCCA
  GCTAAGCATCTACCCCGAGGGACAAGGCAAGCACACACTAGGGCAGCTGGGCCATCCTGG
  CCCTAATCCCTCCAGCGGTGTCCACACTGAGCATTGCAGCACTTGTAAAAGGTGGTCATC
  GGCTCATCTGCAGAGCGGGTCTGAAGCTGCATGAAGTAAGCACGAGGATGTTCGCATTTG
  GGACACGACTCTGCAGTAGAGTCAACATTCTCCCAGGCAGCTGCTCCACCAAGCACATCA
  TCCACTTTTTTCAGTTTTGGGTACTTCCGATTTGTTACCTTGCGGGTGATGTTGTGCACG
  TAGGGGCACGTGTTGCAGGCGAAGCGGTGGCAGCGTTGTCCCTCCTCCACGATCAGCCCG
  TTCCCGCAGCCGGGGCAGAACAGCAGCATGGTCTCGAACTCCGCAGGCTCCAACTCCCGG
  CAGCCTTGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGT
  TCACTAAACGAGCTCTGCTTTATAGACCTCCACCGTACACGCCTACCGCCATTTGGGTCA
  ACGGGCGGGGTTATACGACATTTGGGAAGTCCGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016310
• Insert Size: 820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016310.2, NP_057394.1
• RefSeq Size: 796 bp
• RefSeq ORF: 327 bp
• Locus ID: 51728
• UniProt ID: Q9Y2Y1
• Cytogenetics: 16p13.3
• Domains: TFIIS, RNA_POL_M_15KD
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010]