IARS2 (NM_018060) Human Untagged Clone

CAT#: SC113746

IARS2 (untagged)-Human isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IARS2
• Synonyms: CAGSSS; ILERS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018060, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGTTGGGGGCTGCGCCCTCGCGGGCCGGGCGCGGCCGCCCTGGCCACTGCCCGAAGTTTGTGGGGGA
  CGCCCCGCCTTCCCTGCAGCCCGGGATGGCAAGGGGCGACGAAGAGGCTTCTGGTGCGGTCGGTCTCCGG
  GGCCAGTAACCACCAGCCGAACTCGAATAGTGGCAGATACCGGGACACGGTGCTGCTGCCGCAGACGAGC
  TTCCCCATGAAGCTGCTGGGCCGCCAGCAGCCGGACACGGAGCTGGAGATCCAGCAGAAATGTGGATTTT
  CAGAACTTTATTCATGGCAAAGAGAAAGAAAAGTAAAGACAGAATTTTGCCTTCATGATGGACCTCCTTA
  TGCAAACGGTGACCCTCATGTTGGACATGCTTTAAATAAGATTTTGAAAGACATAGCCAATCGATTCCAT
  ATGATGAATGGCTCCAAAATACATTTTGTGCCCGGCTGGGATTGTCATGGGTTGCCCATTGAAATAAAAG
  TATTATCAGAACTTGGTAGAGAAGCTCAGAATCTTTCAGCTATGGAAATTAGAAAGAAAGCTAGATCATT
  TGCTAAAGCAGCCATTGAGAAACAGAAATCAGCATTTATTCGTTGGGGAATAATGGCAGATTGGAATAAT
  TGCTACTATACATTTGATGGGAAGTATGAAGCCAAACAGTTGAGAACTTTTTACCAAATGTATGATAAGG
  GCTTGGTTTATCGATCTTACAAACCTGTGTTTTGGTCTCCGTCATCTAGGACTGCATTGGCTGAAGCAGA
  ACTTGAATATAATCCTGAGCATGTCAGTCGTTCAATATATGTAAAATTTCCTCTCTTAAAGCCTTCTCCA
  AAATTGGCATCTCTTATAGATGGTTCATCTCCTGTTAGTATTTTGGTCTGGACCACACAACCTTGGACGA
  TTCCAGCCAATGAAGCTGTTTGCTATATGCCTGAATCAAAGTATGCTGTTGTGAAATGTTCTAAGTCTGG
  AGACCTCTACGTACTGGCGGCAGATAAAGTAGCATCTGTTGCTTCTACTTTGGAAACAACATTTGAGACT
  ATTTCAACACTTTCAGGTGTAGATTTGGAAAATGGTACTTGCAGTCATCCATTAATTCCTGATAAAGCCT
  CTCCTCTTTTACCTGCAAATCATGTGACCATGGCAAAAGGAACGGGATTGGTTCACACAGCCCCAGCTCA
  TGGTATGGAAGACTACGGTGTAGCGTCTCAGCACAACCTGCCCATGGATTGTCTAGTGGACGAAGATGGA
  GTTTTCACAGATGTTGCAGGTCCTGAACTTCAAAACAAGGCTGTCCTTGAAGAGGGAACTGATGTGGTTA
  TAAAGATGCTTCAGACTGCAAAGAATTTGTTGAAAGAGGAGAAATTGGTGCATAGCTATCCGTATGACTG
  GAGGACCAAGAAACCTGTGGTTATTCGTGCCAGCAAGCAGTGGTTTATAAACATCACGGATATTAAGACT
  GCAGCCAAGGAATTGTTAAAAAAGGTGAAATTTATTCCTGGATCAGCACTGAATGGCATGGTTGAAATGA
  TGGACAGGCGGCCATATTGGTGTATATCAAGGCAAAGAGTTTGGGGTGTTCCAATTCCTGTGTTTCATCA
  TAAGACCAAGGATGAATACTTGATCAACAGCCAAACCACTGAGCATATTGTTAAACTAGTGGAACAACAC
  GGCAGTGATATCTGGTGGACTCTTCCCCCTGAACAACTTCTTCCAAAAGAAGTCTTATCTGAGGTTGGTG
  GCCCTGATGCCTTGGAATATGTGCCAGGTCAGGATATTTTGGACATCTGGTTTGATAGCGGAACTTCATG
  GTCTTATGTTCTTCCAGGTCCTGACCAAAGAGCAGATTTGTACTTGGAAGGAAAAGACCAGCTCGGGGGT
  TGGTTTCAGTCATCCTTATTAACAAGTGTGGCAGCAAGGAAGAGAGCACCTTATAAGACAGTGATTGTTC
  ATGGATTTACCCTTGGAGAAAAGGGAGAAAAGATGTCCAAGTCTCTTGGGAATGTCATTCATCCTGATGT
  TGTCGTTAATGGAGGACAAGATCAAAGCAAAGAGCCTCCGTATGGTGCTGATGTCCTTCGCTGGTGGGTA
  GCTGATTCCAATGTCTTCACCGAAGTTGCAATTGGCCCATCCGTGCTCAATGCTGCCAGAGATGATATTA
  GCAAGCTTAGGAATACACTTCGCTTTCTTTTGGGAAATGTGGCTGATTTCAACCCAGAAACAGATTCCAT
  CCCTGTAAACGATATGTATGTCATAGACCAGTACATGCTACACTTACTGCAGGATTTGGCAAACAAGATT
  ACCGAATTATACAAACAATATGATTTTGGAAAAGTTGTTCGGCTGTTACGGACGTTTTATACCAGAGAGC
  TCTCTAACTTTTATTTCAGTATAATCAAAGATAGGCTCTATTGTGAAAAGGAAAATGACCCCAAACGACG
  CTCTTGTCAGACTGCATTAGTTGAAATTTTGGATGTAATAGTTCGTTCTTTTGCTCCCATTCTTCCTCAC
  CTGGCTGAAGAGGTGTTCCAGCACATACCTTATATTAAAGAGCCCAAGAGTGTTTTCCGTACTGGGTGGA
  TTAGTACTAGTTCTATCTGGAAAAAGCCCGGGTTGGAAGAAGCTGTGGAGAGTGCGTGTGCAATGCGAGA
  CTCATTTCTTGGAAGCATCCCTGGCAAAAATGCAGCTGAGTACAAGGTTATCACTGTGATAGAACCTGGA
  CTGCTTTTTGAGATAATAGAGATGCTGCAGTCTGAAGAGACTTCCAGCACCTCTCAGTTGAATGAATTAA
  TGATGGCTTCTGAGTCAACTTTACTGGCTCAGGAACCACGAGAGATGACTGCAGATGTAATCGAGCTTAA
  AGGGAAATTCCTCATCAACTTAGAAGGTGGTGATATTCGTGAAGAGTCTTCCTATAAAGTAATTGTCATG
  CCGACTACGAAAGAAAAATGCCCCCGTTGTTGGAAGTATACAGCGGAGTCTTCAGATACACTGTGTCCTC
  GATGTGCAGAAGTTGTCAGTGGAAAATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018060 unedited
  AGGGCGGCCACGACATTCGCACGAGGTCTTACTCGGCTCCCCTTGGTTTCCTGGGGTCCT
  GCCCCTTCAAGCTGGGGCGGGAGCGGAGGACCCCGCTCTCAGGGGTTGCCGGACCATGCG
  TTGGGGGCTGCGCCCTCGCGGGCCGGGCGCGGCCGCCCTGGCCACTGCCCGAAGTTTGTG
  GGGGATCGCTCCTCCTTCCCTGCAGCCCGGGATGGCAAGGGGCGACGAAGAGGCTTCTGG
  TGCGGTCGGTCTCCGGGGCCAGTAACCACCAGCCGAACTCGAATAGTGGCAGATACCGGG
  ACACGGTGCTGCTGCCGCAGACGAGCTTCCCCATGAAGCTGCTGGGCCGCCAGCAGCCGG
  ACACGGAGCTGGAGATCCAGCAGAAATGTGGATTTTCAGAACTTTATTCATGGCAAAGAG
  AAAGAAAAGTAAAGACAGAATTTTGCCTTCATGATGGACCTCCTTATGCAAACGGTGACC
  CTCATGTTGGACATGCTTTAAATAAGATTTTGAAAGACATAGCCAATCGATTCCATATGA
  TGAATGGCTCCAAAATACATTTTGTGCCCGGCTGGGATTGTCATGGGTTGCCCATTGAAA
  TAAAAGTATTATCAGAACTTGGTAGAGAAGCTCAGAATCTTTCAGCTATGGAAATTAGAA
  AGAAAGCTAGATCATTTGCTAAAGCAGCCATTGAGAAACAGAAATCAGCATTTATTCGTT
  GGGGAATAATGGCAGATTGGAATAATTGCTACTATACATTTGATGGGAAGTATGAAGCCC
  AAACAGTTGAGACTTTTTACCAAATGTATGATAAGGGCTTGGTTTATCGATCTTACAAAC
  CTGTGTTTGTCTCCGTCATCTAGGACTGCATTGGCTGAAGCAGAACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018060 unedited
  CGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATTTTTCCAGAATGCCTTTAT
  TTTCAGTATCATAGAATTTAAATACAGAGTCAAAAGATGATTTATAAAATATAAAACATT
  TTCTGCTTGGCCGTATTTGAAGACAAGCTGAATACATATCTATGTTCTGAATAAGTCCAC
  TATGGATATATATAGGAAGAGATATACATATATCCATCCACAGATACACACACACATATA
  TATTTCTGCATGTATATATACATAATTCTTTCTATAGTTACAGGAAATACTTCTTCTATA
  ATTCTGATTTTGACTCCCATCCTCCACCATTTACTCATCCACTCATTACCTAAATCTTGG
  CTTTCTTTCCTATATTGTAAATAATCCATCCAAACTTCTAGCCAGTACTGTCAGGAGGGT
  TCTTGCTCGAGTGAGCTGTTAATACTATTTTCCACTGACAACTTCTGCACATCGAGGACA
  CAGTGTATCTGAAGACTCCGCTGTATACTTCCAACAACGGGGGCATTTTTCTTTCGTAGT
  CGGCATGACAATTACTTTATAGGAAGACTCTTCACGAATATCACCACCTTCTAAGTTGAT
  GAGGAATTTCCCTTTAAGCTCGATTACATCTGCAGTCATCTCTCGTGGTTCCTGAGCCAG
  TAAAGTTGACTCAGAAGCCATCATTAATTCATTCAACTGAGAGGTGCTGGAAGTCTCTTC
  AGACTGCAGCATCTCTATTATCTCAAAAAGCAGTCCAGGTTCTATCACAGTGATAACCTT
  GTACTCAGCTGCATTTTTGCCAGGNATGCTTCCAAGAAATGAGTTTCGCATTGCACACGC
  ACTTCTCACAGGTTCTTTCAACCCGGGCTTTTTCCAGATAGAACTAGNCCTAATCCCCCC
  ATACGGAAAACACCTCTGGGCTTTTATTAAAGGTATGTGCTGGAACACCTTTCANCCCGT
  GAGGAGAATGGAGCTAAGACGATCTTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018060
• Insert Size: 3410 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018060.3, NP_060530.3
• RefSeq Size: 3549 bp
• RefSeq ORF: 3039 bp
• Locus ID: 55699
• UniProt ID: Q9NSE4
• Cytogenetics: 1q41
• Domains: tRNA-synt_1
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• Gene Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014]