HLA-DRA (NM_019111) Human Untagged Clone

CAT#: SC113252

HLA (untagged)-Human major histocompatibility complex, class II, DR alpha (HLA-DRA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA-DRA
• Synonyms: HLA-DRA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_019111, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCATAAGTGGAGTCCCTGTGCTAGGATTTTTCATCATAGCTGTGCTGATGAGCGCTCAGGAATCAT
  GGGCTATCAAAGAAGAACATGTGATCATCCAGGCCGAGTTCTATCTGAATCCTGACCAATCAGGCGAGTT
  TATGTTTGACTTTGATGGTGATGAGATTTTCCATGTGGATATGGCAAAGAAGGAGACGGTCTGGCGGCTT
  GAAGAATTTGGACGATTTGCCAGCTTTGAGGCTCAAGGTGCATTGGCCAACATAGCTGTGGACAAAGCCA
  ACCTGGAAATCATGACAAAGCGCTCCAACTATACTCCGATCACCAATGTACCTCCAGAGGTAACTGTGCT
  CACAAACAGCCCTGTGGAACTGAGAGAGCCCAACGTCCTCATCTGTTTCATAGACAAGTTCACCCCACCA
  GTGGTCAATGTCACGTGGCTTCGAAATGGAAAACCTGTCACCACAGGAGTGTCAGAGACAGTCTTCCTGC
  CCAGGGAAGACCACCTTTTCCGCAAGTTCCACTATCTCCCCTTCCTGCCCTCAACTGAGGACGTTTACGA
  CTGCAGGGTGGAGCACTGGGGCTTGGATGAGCCTCTTCTCAAGCACTGGGAGTTTGATGCTCCAAGCCCT
  CTCCCAGAGACTACAGAGAACGTGGTGTGTGCCCTGGGCCTGACTGTGGGTCTGGTGGGCATCATTATTG
  GGACCATCTTCATCATCAAGGGATTGCGCAAAAGCAATGCAGCAGAACGCAGGGGGCCTCTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019111 unedited
  GTTAGATTTGTATACGACTTACTATAGGCGGCCGCGACTTCGGCACGAGGCCTCGTGCCG
  AATTCGGCACGAGGCCTCACTCCCGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGA
  AAATGGCCATAAGTGGAGTCCCTGTGCTAGGATTTTTCATCATAGCTGTGCTGATGAGCG
  CTCAGGAATCATGGGCTATCAAAGAAGAACATGTGATCATCCAGGCCGAGTTCTATCTGA
  ATCCTGACCAATCAGGCGAGTTTATGTTTGACTTTGATGGTGATGAGATTTTCCATGTGG
  ATATGGCAAAGAAGGAGACGGTCTGGCGGCTTGAAGAATTTGGACGATTTGCCAGCTTTG
  AGGCTCAAGGTGCATTGGCCAACATAGCTGTGGACAAAGCCAACCTGGAAATCATGACAA
  AGCGCTCCAACTATACTCCGATCACCAATGTACCTCCAGAGGTAACTGTGCTCACGAACA
  GCCCTGTGGAACTGAGAGAGCCCAACGTCCTCATCTGTTTCATAGACAAGTTCACCCCAC
  CAGTGGTCAATGTCACGTGGCTTCGAAATGGAAAACCTGTCACCACAGGAGTGTCAGAGA
  CAGTCTTCCTGCCCAGGGAAGACCACCTTTTCCGCAAGTTCCACTATCTCCCCTTCCTGC
  CCTCAACTGAGGACGTTTACGACTGCAGGNTGGAGCACTGGGGCTTGGATGAGCCTCTTC
  TAAGCACTGGGAGTTTGATGCTCCAAGCCCTCTCCCAGAGACTACAGAGAACGTGGNTGT
  GTGCCCTGGGCCTGACTGGGGGTCTGCTGGGCATCATTATTGGGACCCATCTCATCATCA
  GGGGATTGCGCAAAAGCATGCAGCAGACGCAGGGGCCTCTGTAGGACCATGAGTGGATAG
  GGTTTCTTAAAAAGAGATACTGGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019111 unedited
  CGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTCCACCCAAAATCTTCAAA
  ATAGTTTATTGTGTTCAGAAAAATCACAGTTTGAGGAAACCTTAAACAATAAGTACACAA
  GAGATGCCCCATGGGGCATTCCATAGCAGAGACAGACTCCTGTATGTTTTATTCCAGAGG
  CATTGCATGGTGATAATAAAATGATAGGAAATAGAGGAAAATAGATACAGGAAAAGGCAA
  TAGACAGGGAAGCCAGCTAGATGTTAGAGTATGGAGCAATCGAGGAGGCATAACCACTCT
  TGGGGTAGCTATAGGGCTGGAAAATGCTGAAGATGACTGCTTTCACTGAGGTCAAGGATT
  GTAATATTGCCAGCTTTGTAAAGCCATTAAAGCAGAAGTTTCTTCAGTGATCTTCTCTCT
  AAGAAACACCATCACCTCCATGTGCCTTACAGAGGCCCCCTGCGTTCTGCTGCATTGCTT
  TTGCGCAATCCCTTGATGATGAAGATGGTCCCAATAATGATGCCCACCAGACCCACAGTC
  AGGCCCAGGGCACACACCACGTTCTCTGTAGTCTCTGGGAGAGGGCTTGGAGCATCAAAC
  TCCCAGTGCTTGAGAAGAGGCTCATCCAAGCCCCAGTGCTCCACCCTGCAGTCGTAAACG
  TCCTCAGTTGAGGGCAGGAAGGGGAGATAGTGGAACTTGCGGAAAAGGTGGTCTTCCCTG
  GGCAGGAAGACTGTCTCTGACACTCCTGTGGTGACAGGTTTTCCATTTCGAAGCCACGTG
  ACATTGACCACTGGTGGGNGTGAACCTGTCTATGAAACAGATGAGGACGTTGGGCTCTCT
  CAGTCCACAGGGNCTGTCGTGAGACAGNTACCTCTGGAGTACATTGGTGGATCGAGTATA
  TTGGAGCGCTTGTCATGATTCAGTTGGCTTGTCCCACTTATGTGCCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_019111
• Insert Size: 1340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019111.3, NP_061984.2
• RefSeq Size: 1267 bp
• RefSeq ORF: 765 bp
• Locus ID: 3122
• UniProt ID: P01903
• Cytogenetics: 6p21.32
• Domains: MHC_II_alpha, ig, IGc1
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Hematopoietic cell lineage, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq, Aug 2020]