CKMT1B (NM_020990) Human Untagged Clone

CAT#: SC113062

CKMT1B (untagged)-Human creatine kinase, mitochondrial 1B (CKMT1B), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CKMT1B
• Synonyms: CKMT; CKMT1; UMTCK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020990, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGGTCCCTTCTCCCGTCTGCTGTCCGCCCGCCCGGGACTCAGGCTCCTGGCTTTGGCCGGAGCGG
  GGTCTCTAGCCGCTGGGTTTCTGCTCCGACCGGAACCTGTACGAGCTGCCAGTGAACGACGGAGGCTGTA
  TCCCCCGAGCGCTGAGTACCCAGACCTCCGAAAGCACAACAACTGCATGGCCAGTCACCTGACCCCAGCA
  GTCTATGCACGGCTCTGCGACAAGACCACACCCACTGGTTGGACGCTAGATCAGTGTATCCAGACTGGCG
  TGGACAACCCTGGCCACCCCTTCATCAAGACTGTGGGCATGGTGGCTGGAGATGAGGAGACCTATGAGGT
  ATTTGCTGACCTGTTTGACCCTGTGATCCAAGAGCGACACAATGGATATGACCCCCGGACAATGAAGCAC
  ACCACGGATCTAGATGCCAGTAAAATCCGTTCTGGCTACTTTGATGAGAGGTATGTATTGTCCTCTAGAG
  TCAGAACTGGCCGAAGCATCCGAGGACTCAGTCTGCCTCCAGCTTGCACTCGAGCAGAGCGACGAGAGGT
  GGAACGTGTTGTGGTGGATGCACTGAGTGGCCTGAAGGGTGACCTGGCTGGACGTTACTATAGGCTCAGT
  GAGATGACAGAGGCTGAACAGCAGCAGCTTATTGATGACCACTTTCTGTTTGATAAGCCTGTGTCCCCGT
  TGCTGACTGCAGCAGGAATGGCTCGAGACTGGCCAGATGCTCGTGGAATTTGGCACAACAATGAGAAGAG
  CTTCCTGATCTGGGTGAATGAGGAGGATCATACACGGGTGATCTCCATGGAGAAGGGTGGTAACATGAAG
  AGAGTGTTTGAAAGATTCTGCCGAGGCCTCAAAGAGGTGGAGAGACTTATCCAAGAACGTGGCTGGGAGT
  TCATGTGGAATGAGCGTTTGGGATACATCTTGACCTGTCCATCTAACCTGGGCACTGGACTTCGGGCAGG
  AGTGCACATCAAACTGCCCCTGCTAAGCAAAGATAGCCGCTTCCCAAAGATCCTGGAGAACCTAAGACTC
  CAAAAACGTGGTACTGGAGGAGTGGACACTGCTGCTACAGGCGGTGTCTTTGATATTTCTAATTTGGACC
  GACTAGGCAAATCAGAGGTGGAGCTGGTGCAACTGGTCATCGATGGAGTAAACTATTTGATTGATTGTGA
  ACGGCGTCTGGAGAGAGGCCAGGATATCCGCATCCCCACACCTGTCATCCACACCAAGCATTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020990 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCCATTCCGGCTCC
  AGCCTCCAATCCGACCCCCATTTCGGCTGCAGCCTCGGACCTAGCTCCGGCCCTCGGTCT
  ATCCGGTTGCATCCTCCCTCCCTGTTCCGGATCTTATCTTGCGCCAGCGCCTACTCCAGG
  ATCCCGTAGCCAGACCTCAAGCCATGGCTGGTCCCTTCTCCCGTCTGCTGTCCGCCCGCC
  CGGGACTCAGGCTCCTGGCTTTGGCCGGAGCGGGGTCTCTAGCCGCTGGGTTTCTGCTCC
  GACCGGAACCTGTACGAGCTGCCAGTGAACGACGGAGGCTGTATCCCCCGAGCGCTGAGT
  ACCCAGACCTCCGAAAGCACAACAACTGCATGGCCAGTCACCTGACCCCAGCAGTCTATG
  CACGGCTCTGCGACAAGACCACACCCACTGGTTGGACGCTAGATCAGTGTATCCAGACTG
  GCGTGGACAACCCTGGCCACCCCTTCATCAAGACTGTGGGCATGGTGGCTGGAGATGAGG
  AGACCTATGAGGTATTTGCTGACCTGTTTGACCCTGTGATCCAAGAGCGACACAATGGAT
  ATGACCCCCGGACAATGAAGCACACCACGGATCTAGATGCCAGTAAAATCCGTTCTGGCT
  ACTTTGATGAGAGGTATGTATTGTCCTCTAGAGTCAGAACTGGCCGAAGCATCCGAGGAC
  TCAGTCTGCCTCCAGCTTGCACTCGAGCAGAGCGACGAGAGGTGGAACGTGTTGTGGNTG
  ATGCACTGAGTGGCCTGAAGGGTGACCTGGCTGGNACGTACTATAGGCTCAGTGAGATGA
  CAGAGGGCTGACAGCAGCAGCTTATTGATGACCACTNTCTNTNGATAAGCCTGTGTNCCC
  CGTGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020990 unedited
  CTATGGACCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAACCAAAACAGGGGGTTT
  TTACTAGGATGGGGGCAGGGAATGCGGGGCCAGGCCCAAACCAAGAAAAAGGGCCCTTTT
  TTAAAAGGACCAAACCCCCGGGAAATTTTGAGCCATCACCTGGCAAGGGGGATTAAATGC
  TTGGGGGGGAAAACAGGGGGGGGAATGCGAATTTCCGGGCCTTTTTCCAAACCCCGTTAA
  AAATAAATAAAATAGTTAACTCCTTGGAGGACCAGTTGCCCCACCCCCCCCTTTGATTTG
  CCTAGCCGGCCCAAATTAAAAATATAAAAAACCCCCCCTGTACAAAAAGGGCCCACTCCT
  CCAAACCCACTTTTTGGGAGTTTAAGGTTCCCCAGAATTTTTGGAAAACGGTTTTTTTTT
  TTTACCAGGGGCATTTTGATGGCCCCTCCTGCCCAAATCCCAGGGCCCAGGTTAAAGGGA
  CAGGGAAAAATGTATCCCAAACGCTATTTCCAAATGAACTCCCCACCCCGTTCTTGGAAA
  AGTCTTTCCCACCTCTTGGGGCCCCCGCAGAATTTTTAAAACTCTCTTTTAATGGTACCC
  CCCCTTTTCATGGGAAAACACCGGGTATTGACCCTCCTAATTCCCCCAAAACAGGAAGTT
  TTTTTAATTGTGTGGCCAAATTCCACGACCATGTGGCCAGTCTTAGACCCATTCTGGTGG
  AATAAGCACCGGGAACCCAGTTTTAACAAACAAAAGGGGCCATAAAAGACTGGTGGTGTA
  AACCCTGTTTTTCCCTGGGCCTTAAGAAACGGCCAGCCGGGCCCCTTTTAGGCCATTAGG
  GGATCACCCCACACAGTCCCCCTTTTTNCCTTTGTTAAAGAGGAAAGGGGGCAAACTGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020990
• Insert Size: 1460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020990.3, NP_066270.1
• RefSeq Size: 1779 bp
• RefSeq ORF: 1254 bp
• Locus ID: 1159
• UniProt ID: P12532
• Cytogenetics: 15q15.3
• Domains: ATP-gua_Ptrans
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]