PPP3CB (NM_021132) Human Untagged Clone

CAT#: SC112977

PPP3CB (untagged)-Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP3CB
• Synonyms: CALNA2; CALNB; CNA2; PP2Bbeta
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021132, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGCCCCGGAGCCGGCCCGGGCTGCACCGCCCCCACCCCCGCCCCCGCCGCCCCCTCCCGGGGCTG
  ACCGCGTCGTCAAAGCTGTCCCTTTCCCCCCAACACATCGCTTGACATCTGAAGAAGTATTTGATTTGGA
  TGGGATACCCAGGGTTGATGTTCTGAAGAACCACTTGGTGAAAGAAGGTCGAGTAGATGAAGAAATTGCG
  CTTAGAATTATCAATGAGGGTGCTGCCATCCTTCGGAGAGAGAAAACCATGATAGAAGTAGAAGCTCCAA
  TCACAGTGTGTGGTGACATCCATGGCCAATTTTTTGATCTGATGAAACTTTTTGAAGTAGGAGGATCACC
  TGCTAATACACGATACCTTTTTCTTGGCGATTATGTGGACAGAGGTTATTTTAGTATAGAGTGTGTCTTA
  TATTTATGGGTTCTGAAGATTCTATACCCAAGCACATTATTTCTTCTGAGAGGCAACCATGAATGCAGAC
  ACCTTACTGAATATTTTACCTTTAAGCAGGAATGTAAAATTAAGTATTCGGAAAGAGTCTATGAAGCTTG
  TATGGAAGCTTTTGATAGTTTGCCTCTTGCTGCACTTTTAAACCAACAGTTTCTTTGTGTTCATGGTGGA
  CTTTCACCAGAAATACACACACTGGATGATATTAGGAGATTAGATAGATTCAAAGAGCCACCTGCATTTG
  GACCAATGTGTGACTTGTTATGGTCCGATCCTTCTGAAGATTTTGGAAATGAAAAATCACAGGAACATTT
  TAGTCACAATACAGTTCGAGGATGTTCTTATTTTTATAACTATCCAGCAGTGTGTGAATTTTTGCAAAAC
  AATAATTTGTTATCGATTATTAGAGCTCATGAAGCTCAAGATGCAGGCTATAGAATGTACAGAAAAAGTC
  AAACTACAGGGTTCCCTTCATTAATAACAATTTTTTCGGCACCTAATTACTTAGATGTCTACAATAATAA
  AGCTGCTGTATTAAAGTATGAAAATAATGTGATGAATATTCGACAGTTTAACTGTTCTCCACATCCTTAC
  TGGTTGCCTAATTTTATGGATGTCTTCACGTGGTCTTTACCGTTTGTTGGAGAAAAAGTGACAGAAATGT
  TGGTAAATGTTCTGAGTATTTGCTCTGATGATGAACTAATGACTGAAGGTGAAGACCAGTTTGATGGTTC
  AGCTGCAGCCCGGAAAGAAATCATAAGAAACAAAATTCGAGCAATTGGCAAGATGGCAAGAGTCTTCTCT
  GTTCTCAGGGAGGAGAGTGAAAGTGTGCTGACACTCAAGGGCCTGACTCCCACAGGGATGTTGCCTAGTG
  GAGTGTTAGCTGGAGGACGGCAGACCCTGCAAAGTGCCACAGTTGAGGCTATTGAGGCTGAAAAAGCAAT
  ACGAGGATTCTCTCCACCACATAGAATCTGCAGTTTTGAAGAGGCAAAGGGTTTGGATAGGATCAATGAG
  AGAATGCCACCTCGGAAAGATGCTGTACAGCAAGATGGTTTCAATTCTCTGAACACCGCACATGCCACTG
  AGAACCACGGGACGGGCAACCATACTGCCCAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021132 unedited
  NGGGGTCGAATATTTGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGAAGC
  CGAGCCCCGGGCCCAGCATGGCCGCCCCGGAGCCGGCCCGGGCTGCACCGCCCCCACCCC
  CGCCCCCGCCGCCCCCTCCCGGGGCTGACCGCGTCGTCAAAGCTGTCCCTTTCCCCCCAA
  CACATCGCTTGACATCTGAAGAAGTATTTGATTTGGATGGGATACCCAGGGTTGATGTTC
  TGAAGAACCACTTGGTGAAAGAAGGTCGAGTAGATGAAGAAATTGCGCTTAGAATTATCA
  ATGAGGGTGCTGCCATCCTTCGGAGAGAGAAAACTATGATAGAAGTAGAAGCTCCAATCA
  CAGTGTGTGGTGACATCCATGGCCAATTTTTTGATCTGATGAAACTTTTTGAAGTAGGAG
  GATCACCTGCTAATACACGATACCTTTTTCTTGGCGATTATGTGGACAGAGGTTATTTTA
  GTATAGAGTGTGTCTTATATTTATGGGTTCTGAAGATTCTATACCCAAGCACATTATTTC
  TTCTGAGAGGCAACCATGAATGCAGACACCTTACTGAATATTTTACCTTTAAGCAGGAAT
  GTAAAATTAAGTATTCGGAAAGAGTCTATGAAGCTTGTATGGAAGCTTTTGATAGTTTGC
  CTCTTGCTGCACTNTTAAACCAACAGTTTCTTTGTGTTCATGGTGGACTCTCACCAGAAA
  TACACACACTGGATGATATTAGGAGATTAGATAGATTCAAAGAGCCACCTGCATTTGGAC
  CAATGTGTGACTTGTTATGGTCCGATCCTTTCTGAGATTTGGAAATGAANAATCACAGGA
  CATTTTAGTCCAATACAGTTCGAGGAGTTCCTATTTTTATACTATCCAGCAGGGTGTGAA
  TT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021132 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGGAGCAGCGGCCCATGCA
  TGGAATTTATTGTGTGCTACTGTTTATAAAATACTCGAATAGTCCTGCACATGCATAATA
  TTTCCAACTTAGACAGGAGACCATACAGGGTGCACTTTCTGGCAAACAAAACAATAGATG
  GTTCCGCTGCCTGGAGCTCTGAGTTGTATTCCAGGGCATGAGGGAAGCAGGCCACCAAAG
  TAAAGGGAAATACCAAACTACAGTGGCAATCAATACAGGTCAATAATTGTGAAAAATTAG
  CACATGGTTCCATTTAGTTTAACCAAGCAGTTCAGTAACTATCAAAAGAAAGGTTTCAAC
  ATGCAGTCTTGACTTTTATGCTTCCACTAACATCTGAATGATTGAACAGAACCATTGCTT
  CTAGGCTGCATTGCTTAATCAAAAACACTTCCAGAACATTTTAAAATTTCCTTTGCATTA
  AAACATAAAAATGATAGAAAGCCAGCAGCAAATCACCCTGGTGGCTCTCTAAGCAACTTG
  AGTACTCACAATAAACTAAAATTTCTCATAACATCTAGGTAACCTATACATGTCGTACCT
  GTACATCATAGGTCTATATATTAAATATTTGCAAAATGAAAACATGCATACACAAAATAC
  GTCAAGTTTACAGACATGACTTGAATTAAAATTTACTTTGACAATGTACAAACTTCTTTT
  AAAGTACCTTAAATGAGCAAATTCTGTAATTCTTCATTAATTTGAAAATTTAGAGGTTTT
  CTTTTTTAAATCTCTTGGGTGTAAGGCCTTAAAGAAATGGGCCCAAAAAAACTCTAATTA
  ACTTTTGGGCTTGCGTTCAAGTAAGAAAACCTGCCTGAAAATAGGATTCCTTTCTTATTA
  AAAAATCCCCACTTTGTCCCAAATACCCCCTTTAG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_021132
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021132.1, NP_066955.1
• RefSeq Size: 3079 bp
• RefSeq ORF: 1575 bp
• Locus ID: 5532
• UniProt ID: P16298
• Cytogenetics: 10q22.2
• Domains: Metallophos, PP2Ac
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:19154138, PubMed:26794871). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform b which is shorter than isoform a.