MASA (ENOPH1) (NM_021204) Human Untagged Clone

CAT#: SC112927

ENOPH1 (untagged)-Human enolase-phosphatase 1 (ENOPH1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MASA
• Synonyms: E1; MASA; MST145; mtnC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112927 sequence for NM_021204 edited (data generated by NextGen Sequencing)
  ATGGTCGTGCTTTCGGTCCCCGCCGAAGTCACCGTGATCCTGTTAGATATCGAAGGTACC
  ACAACCCCGATTGCTTTCGTGAAGGACATTTTATTTCCTTACATCGAAGAAAATGTTAAA
  GAGTATCTGCAGACACATTGGGAAGAAGAGGAGTGCCAGCAGGATGTCAGTCTTTTGAGG
  AAACAGGCTGAAGAGGACGCCCACCTGGATGGGGCTGTTCCTATCCCTGCAGCATCTGGG
  AATGGAGTGGATGATCTGCAACAGATGATCCAGGCCGTGGTAGATAATGTGTGCTGGCAG
  ATGTCCCTGGATCGAAAGACCACTGCACTCAAACAGCTGCAGGGCCACATGTGGAGGGCG
  GCATTCACAGCTGGGCGCATGAAAGCAGAGTTCTTTGCAGATGTAGTTCCAGCAGTCAGG
  AAGTGGAGAGAGGCCGGAATGAAGGTGTACATCTATTCCTCAGGGAGTGTGGAGGCACAG
  AAACTGTTATTCGGGCATTCTACGGAGGGAGATATTCTTGAGCTTGTTGATGGTCACTTT
  GATACCAAGATTGGACACAAAGTAGAGAGTGAAAGTTACCGAAAGATTGCAGACAGCATT
  GGGTGCTCAACCAACAACATTTTGTTTCTGACAGATGTTACTCGAGAGGCCAGTGCTGCT
  GAGGAAGCAGATGTGCACGTAGCTGTGGTGGTGAGACCAGGCAACGCAGGATTAACAGAT
  GATGAGAAGACTTACTACAGCCTCATCACATCCTTCAGTGAACTATACCTGCCTTCCTCA
  ACCTAG
  
  Clone variation with respect to NM_021204.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021204 unedited
  AGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGGTGTGCAG
  AAGTGTCCTCTCCCCACGCGCGGCGGGCTGCACTTGGTCGCTGGCTCCGAGATCGCGCGG
  GGCCGCCGGAAGCCCAAGACGGTACCGGGGGCCGCAGCCGCAGCCGGCGCCGCCCTCCGC
  CCTCCCCAACAGCAGGCCGAGTCCCGTAGCATCCGGTAGGGAAATGGTCGTGCTTTCGGT
  CCCCGCCGAAGTCACCGTGATCCTGTTAGATATCGAAGGTACCACAACCCCGATTGCTTT
  CGTGAAGGACATTTTATTTCCTTACATCGAAGAAAATGTTAAAGAGTATCTGCAGACACA
  TTGGGAAGAAGAGGAGTGCCAGCAGGATGTCAGTCTTTTGAGGAAACAGGCTGAAGAGGA
  CGCCCACCTGGATGGGGCTGTTCCTATCCCTGCAGCATCTGGGAATGGAGTGGATGATCT
  GCAACAGATGATCCAGGCCGTGGTAGATAATGTGTGCTGGCAGATGTCCCTGGATCGAAA
  GACCACTGCACTCAAACAGCTGCAGGGCCACATGTGGAGGGCGGCATTCACAGCTGGGCG
  CATGANAGCAGAGTTCTTTGCAGATGTAGTTCCAGCAGTCANGAAGTGGAGAGAGGCCGG
  AATGAANGTGTACATCTATTCCTCAGGNAGTGNTGANNGCACAGAAACTGNNTATTCGGC
  ATTTCTACGAGGGAGATATTTCTTGAGCTGTTGATGGTCACTTTGATACCCAGATTGGAC
  ACANAGTAGAGAGTGAAAGTTACCGAAAGATGCAGACAGCATTGGGTGCTCAACCACCAC
  ATTTTGTTTCTGACAGATGTTACCTCCAAAGGCCAGTGCTGCTGAAGAAGCAAATGTGCA
  CGTAGCTGTGGTGGTGAAACCAGGCACCGCAGATTACAGATGATGAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021204 unedited
  NNNATTTTTTAGCTTGNACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTT
  TGAAGAAAAAAGCTTTTATTAATCACAACATGAAATTAACATGACATCGTATTAGAATTA
  AACCAGGATAAATACTGCTGTTATAAAATTTACATTTCTCACATCCATTCCAGAGGAAAA
  TCTTACATATTAGGGCTCATCTTAATGTTATGGACTGATTTCAGTAAAACTTTTTAAATA
  GTAAATAGCAATTAACGTATTCTCAAACTGTGCTAAGTAGTTAGAAAGGCAACTATAAAA
  TCTATAATGATAACACGTGGCAGGATTAGAACTGTTCTGTTAAACATTTAAGAACCACAT
  CTTTGTTTTAGATGTTACCTCAGTGCACCTTTTTCACTGGTAAGGCACCTGCTTGCTGTG
  GGTGCCCCATTGTTACCCAGGTTTCTTTTTAGTAAAAACCCTTGGTTGAACAAACCTAAG
  GGAGTTTTCTTTCCCATTTTGGCCTTTGAACCGAAAGGAAACCCTGGGCCTTGGTTTTTT
  AAGGGGGCCATTTGAAACCCCCTGTTTAATTTTTTAATCCACGGGGCCTAAAATTTCCTT
  TCTTTTAAAAAATTCCCCTTTAAAAATTTTATTTTTAAAAAAACGGGCCTTAAAAAAACC
  TCAAACCCCCTTTCCCCTTTTCCCCCCCTTTTTAACCCTCCCCCCCCTAAAGGGGTTTTA
  AAATTTTTTTCACCCACCCCCTTTCCCCCCCTTTGGGGGGAGAAAGATTTTAAAAAAATT
  TCCCCCAAAAAAACCTTTTAAAAAAATTGTGTGGCCCCCAAAAAAAATTTTTTTTTTTTT
  ATCCCCGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021204
• Insert Size: 1990 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021204.1, NP_067027.1
• RefSeq Size: 1992 bp
• RefSeq ORF: 786 bp
• Locus ID: 58478
• UniProt ID: Q9UHY7
• Cytogenetics: 4q21.22
• Domains: Hydrolase
• Protein Pathways: Cysteine and methionine metabolism
• Gene Summary: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).