ELOVL5 (NM_021814) Human Untagged Clone

CAT#: SC112897

ELOVL5 (untagged)-Human ELOVL fatty acid elongase 5 (ELOVL5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELOVL5
• Synonyms: dJ483K16.1; HELO1; SCA38
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC112897 representing NM_021814.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  
  AGCCACCGGTGTCTCCTTCTACATCCGCCTCTGCGCCGGCTGCCACCCGCGCTCCCTCCGCCGCCGCCG
  CCTTGCTGCTGCTCAAAGCTGCTGCCGCCCCTTGGGCTAAAAGGTTTTCAAATGGAACATTTTGATGCA
  TCACTTAGTACCTATTTCAAGGCATTGCTAGGCCCTCGAGATACTAGAGTAAAAGGATGGTTTCTTCTG
  GACAATTATATACCCACATTTATCTGCTCTGTCATATATTTACTAATTGTATGGCTGGGACCAAAATAC
  ATGAGGAATAAACAGCCATTCTCTTGCCGGGGGATTTTAGTGGTGTATAACCTTGGACTCACACTGCTG
  TCTCTGTATATGTTCTGTGAGTTAGTAACAGGAGTATGGGAAGGCAAATACAACTTCTTCTGTCAGGGC
  ACACGCACCGCAGGAGAATCAGATATGAAGATTATCCGTGTCCTCTGGTGGTACTACTTCTCCAAACTC
  ATAGAATTTATGGACACTTTCTTCTTCATCCTGCGCAAGAACAACCACCAGATCACGGTCCTGCACGTC
  TACCACCATGCCTCGATGCTGAACATCTGGTGGTTTGTGATGAACTGGGTCCCCTGCGGCCACTCTTAT
  TTTGGTGCCACACTTAATAGCTTCATCCACGTCCTCATGTACTCTTACTATGGTTTGTCGTCAGTCCCT
  TCCATGCGTCCATACCTCTGGTGGAAGAAGTACATCACTCAGGGGCAGCTGCTTCAGTTTGTGCTGACA
  ATCATCCAGACCAGCTGCGGGGTCATCTGGCCGTGCACATTCCCTCTTGGTTGGTTGTATTTCCAGATT
  GGATACATGATTTCCCTGATTGCTCTCTTCACAAACTTCTACATTCAGACCTACAACAAGAAAGGGGCC
  TCCCGAAGGAAAGACCACCTGAAGGACCACCAGAATGGGTCCATGGCTGCTGTGAATGGACACACCAAC
  AGCTTTTCACCCCTGGAAAACAATGTGAAGCCAAGGAAGCTGCGGAAGGATTGAAGTCAAAGAATTGAA
  ACCCTCCAAACCACGTCATCTGATTGTAAGCACAATATGAGTTGTGCCCCAATGCTCGTTAACAGCTGC
  TGTAACTAGTCTGGCCTACAATAGTGTGATTCATGTAGGACTTCTTTCATCAATTCAAAACCCCTAGAA
  AACGTATACAGATTATATAAGTAGGGATAAGATTTCTAACATTTCTGGGCTCTCTGACCCCTGCGCTAG
  ACTGTGGAAAGGGAGTATTATTATAGTATACAACACTGCTGTTGCCTTATTAGTTATAACATGATAGGT
  GCTGAATTGTGATTCACAATTTAAAAACACTGTAATCCAAACTTTTTTTTTTAACTGTAGATCATGCAT
  GTGATTGGAAATGTAAATTTGTACAATGTTGTTATGGTAGAGAAACACACATGCCTTAAAATTTAAAAA
  GCAGGGCCCAAAGCTTATTAGTTTAAATTAGGGTATGTTTCAAGTTTGTATTAATTTGTAATAGCTCTG
  TTTAGAAAAAATCAAAGACCATGATTTATGAAACTAATGTGACATAATTTCCAGTGACTTGTTGATGTG
  AAATCAGACACGGCACCTTCAGTTTTGTACTATTGGCTTTGAATCAAGCAGGCTCAAATCTAGTGGAAC
  AGTCAGTTTAACTTTTTAACAGATCTTATTTTTTTATTTTGAGTGCCACTATTAATGTAAAAAGGGGGG
  GGCTCTACAGCAGTCGTGATGAAACTTAAATATATATTCTTTGTCCTCGAGATTTTAGGAAGGGTGTAG
  GGTGAGTAGGCCATTTTTAATTTCTGAAGTGCTAAGTGTTTTTATACAGCAAACAAAAAGTCAATTTTG
  CTTTCCACCAGTGCGAGAGAGGATGTATACTTTTCAAGAGAGATGATTGCCTATTTACCGTTTGACAGA
  GTCCCGTAGATGAGCAATGGGGAACTTGTTGCCAGGGTCTAAATTTGGATTGATTTATGCACTGTTATC
  TGTTTTGACACAGATTTCCTTGTAAAATGTGCCTAGTTTACCAAAATTAACAAAGGGGGGGAAAGGACC
  TTAGAACTTTTTAAGGTAAAATCAAATATAGCTACAGCATAAGAGAATCGAGAAATTTGATAGAGGTAA
  CTTGTTTAATGTAAATCTAATAGTACTTGTAATTTCTTTCTGCTTAGAATCTAAAGATGTGTTTAGAAC
  CTCTTGTTTAAAAATAATAGACTGCTTATCATAAAATCACATCTCACACATTTGAGGCAGTGGTCAAAC
  AGGTAAAGCCTATGATGTGTGTCATTTTAAAGTGTCGGAATTTAGCCTCTGAATACCTTCTCCATTGGG
  GGAAAGATATTCTTGGAACCACTCATGACATATCTTAGAAGGTCATTGACAATGTATAAACTAATTGTT
  GGTTTGATATTTATGTAAATATCAGTTTACCATGCTTTAATTTTGCACATTCGTACTATAGGGAGCCTA
  TTGGTTCTCTATTAGTCTTGTGGGTTTTCTGTTTGAAAAGGAGTCATGGCATCTGTTTACATTTACCTT
  ATCAAACCTAGAATGTGTATATTTATAAATGTATGTCTTCATTGCTAGGTACTAATTTGCAGATGTCTT
  TACATATTTCAATACAGAAACTATAACATTCAATAGTGTGCTGTCAAAGTGTGCTTAGCTCACCTGGAT
  ATACCTACATTGTTAAATGTCTAAACAGTAATCATTAAAACATTTTTGATTAAAAAAAAAAAAAAAAAA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_021814
• Insert Size: 2760 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_021814.3.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021814.3
• RefSeq Size: 3003 bp
• RefSeq ORF: 2760 bp
• Locus ID: 60481
• UniProt ID: Q9NYP7
• Cytogenetics: 6p12.1
• Domains: ELO
• Protein Families: Transmembrane
• Protein Pathways: Biosynthesis of unsaturated fatty acids
• MW: 106.9 kDa
• Gene Summary: This gene belongs to the ELO family. It is highly expressed in the adrenal gland and testis, and encodes a multi-pass membrane protein that is localized in the endoplasmic reticulum. This protein is involved in the elongation of long-chain polyunsaturated fatty acids. Mutations in this gene have been associated with spinocerebellar ataxia-38 (SCA38). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Variants 1 and 5 encode the same isoform.