Fibrinogen beta chain (FGB) (NM_005141) Human Untagged Clone

CAT#: SC112673

FGB (untagged)-Human fibrinogen beta chain (FGB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Fibrinogen beta chain
• Synonyms: HEL-S-78p
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005141, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAAAGGATGGTTTCTTGGAGCTTCCACAAACTTAAAACCATGAAACATCTATTATTGCTACTATTGT
  GTGTTTTTCTAGTTAAGTCCCAAGGTGTCAACGACAATGAGGAGGGTTTCTTCAGTGCCCGTGGTCATCG
  ACCCCTTGACAAGAAGAGAGAAGAGGCTCCCAGCCTGAGGCCTGCCCCACCGCCCATCAGTGGAGGTGGC
  TATCGGGCTCGTCCAGCCAAAGCAGCTGCCACTCAAAAGAAAGTAGAAAGAAAAGCCCCTGATGCTGGAG
  GCTGTCTTCACGCTGACCCAGACCTGGGGGTGTTGTGTCCTACAGGATGTCAGTTGCAAGAGGCTTTGCT
  ACAACAGGAAAGGCCAATCAGAAATAGTGTTGATGAGTTAAATAACAATGTGGAAGCTGTTTCCCAGACC
  TCCTCTTCTTCCTTTCAGTACATGTATTTGCTGAAAGACCTGTGGCAAAAGAGGCAGAAGCAAGTAAAAG
  ATAATGAAAATGTAGTCAATGAGTACTCCTCAGAACTGGAAAAGCACCAATTATATATAGATGAGACTGT
  GAATAGCAATATCCCAACTAACCTTCGTGTGCTTCGTTCAATCCTGGAAAACCTGAGAAGCAAAATACAA
  AAGTTAGAATCTGATGTCTCAGCTCAAATGGAATATTGTCGCACCCCATGCACTGTCAGTTGCAATATTC
  CTGTGGTGTCTGGCAAAGAATGTGAGGAAATTATCAGGAAAGGAGGTGAAACATCTGAAATGTATCTCAT
  TCAACCTGACAGTTCTGTCAAACCGTATAGAGTATACTGTGACATGAATACAGAAAATGGAGGATGGACA
  GTGATTCAGAACCGTCAAGACGGTAGTGTTGACTTTGGCAGGAAATGGGATCCATATAAACAGGGATTTG
  GAAATGTTGCAACCAACACAGATGGGAAGAATTACTGTGGCCTACCAGGTGAATATTGGCTTGGAAATGA
  TAAAATTAGCCAGCTTACCAGGATGGGACCCACAGAACTTTTGATAGAAATGGAGGACTGGAAAGGAGAC
  AAAGTAAAGGCTCACTATGGAGGATTCACTGTACAGAATGAAGCCAACAAATACCAGATCTCAGTGAACA
  AATACAGAGGAACAGCCGGTAATGCCCTCATGGATGGAGCATCTCAGCTGATGGGAGAAAACAGGACCAT
  GACCATTCACAACGGCATGTTCTTCAGCACGTATGACAGAGACAATGACGGCTGGTTAACATCAGATCCC
  AGAAAACAGTGTTCTAAAGAAGACGGTGGTGGATGGTGGTATAATAGATGTCATGCAGCCAATCCAAACG
  GCAGATACTACTGGGGTGGACAGTACACCTGGGACATGGCAAAGCATGGCACAGATGATGGTGTAGTATG
  GATGAATTGGAAGGGGTCATGGTACTCAATGAGGAAGATGAGTATGAAGATCAGGCCCTTCTTCCCACAG
  CAATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005141 unedited
  NCGGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGACATGG
  CAAAGCATGGCACAGATGATGGTGTAGTATGGATGAATTGGCTGCATGACATCTATTATA
  CCACCATCCACCACCGTCTTCTTTAGAACACTGTTTTCTGGGATCTGATGTTAACCAGCC
  GTCATTGTCTCTGTCATACGTGCTGAAGAACATGCCGTTGTGAATGGTCATGGTCCTGTT
  TTCTCCCATCAGCTGAGATGCTCCATCCATGAGGGCATTACCGGCTGTTCCTCTGTATTT
  GTTCACTGAGATCTGGTATTTGTTGGCTTCATTCTGTACAGTGAATCCTCCATAGTGAGC
  CTTTACTTTGTCTCCTTTCCAGTCCTCCATTTCTATCAAAAGTTCTGTGGGTCCCATCCT
  GGTAAGCTGGCTAATTTTATCATTTCCAAGCCAATATTCACCTGGTAGGCCACAGTAATT
  CTTCCCATCTGTGTTGGTTGCAACATTTCCAAATCCCTGTTTATATGGATCCCATTTCCT
  GCCAAAGTCAACACTACCGTCTTGACGGTTCTGGTTTCTTGGAGCTTCCACAAACTTAAA
  ACCATGAAACATCTATTATTGCTACTATTGTGTGTTTTTCTAGTTAAGTCCCAAGGTGTC
  AACGACAATGAGGAGGGTTTCTTCAGTGCCCGTGGTCATCGACCCCTTGACAAGAAGAGA
  GAGAAGCTCCCAGCCTGAGGCCTGCCCCACCGCCCATCAGTGGGAGTGGCTATCGGGCTC
  GTCCAGCCAAGCAGCTGCCACTCAAAANAAGTAGAAAGAAAAGCCCTGATGCTGNAGGCT
  GTCTTCACGCTGACCCANACCTGGGGGTGTTGTGTCCTACAGGATGTCAGTTGCAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005141 unedited
  NTCTTACTTGNCCGCGGCCGCAATCTANNGATCGGTTTTTTTTTTTTTTTTTTTTTATTG
  CAACAAAGTCTGGTTTAATTAATAAATCTTTTTGGAAGTTTTCTTTGACAGATCCTACAT
  AAATAAATATAGTTTTAAAAGTGGACTTTTAAACATCAGTTTTTCCTTTCCGTCATGGTT
  TTCCTACGACAAGGATAAAAGACCCCTCTTCCTTATTTGAAAATTCTCGCAATCAACTAG
  AACTGAAATTATGAACACCAACTCTGTCAAAACTATACTTTTGTTACTTCTTGGTGAGCA
  AGAGAAATGAAGAAAACAAAAGAAAATCATGTGCTATTTTAATGTAATTTATCCTATACT
  TTTTTCAAAAAGTCACACTCACGTCTGCTTGAGAGTTTTAGAGGAATATAATGTATGAAA
  GAAAATTCCAATAACATAATGTACAAAAATGTTGTCACATACAGAAGAGCAAAAATCTAC
  GTATTGGGGACTATTGCTGTGGGAAGAAGGGCCTGATCTTCATACTCATCTTCCTCATTG
  AGTACCATGACCCCTTCCAATTCATCCATACTACACCATCATCTGTGCCATGCTTTGCCA
  TGTCCCAGGTGTACTGTCCACCCCAGTAGATCTGCTGAAATAGGGNNCCCCCCTTCCNCC
  ACTTATTTTACCCACCCTTCCCACCCCGGTTTTTTTTAAAACACTGTTTTCTTGGGATCT
  GATGTTAACCCAGCCGTCATTGTCTCTGGCATACGTGCTGAAGAACATGCCTTGTGAATG
  GCATGGCCCTGTTTTCTCCCTCAGCTGAGATGCTCATCCATGAGGCATTACCGGCTGTTC
  CTCTGTATTTGTCACTGAAATCTGTTATTGTTGGCTTCTTCTGTACATGAATCCTCCATA
  NGAGCCTTTACTTGNCTCCTTTCAGCCTCCTTTCTATCAAAGTCTGGGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005141
• Insert Size: 2570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005141.2, NP_005132.2
• RefSeq Size: 1949 bp
• RefSeq ORF: 1476 bp
• Locus ID: 2244
• UniProt ID: P02675
• Cytogenetics: 4q31.3
• Domains: FBG
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Fibrinogen serves key roles in hemostasis and antimicrobial host defense. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.