GMPPA (NM_013335) Human Untagged Clone

CAT#: SC112661

GMPPA (untagged)-Human GDP-mannose pyrophosphorylase A (GMPPA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GMPPA
• Synonyms: AAMR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112661 sequence for NM_013335 edited (data generated by NextGen Sequencing)
  ATGCTCAAAGCGGTGATCCTGATTGGAGGCCCTCAAAAGGGAACTCGCTTCAGACCTTTG
  TCTTTTGAGGTGCCCAAACCATTGTTTCCTGTGGCAGGGGTCCCTATGATCCAACACCAT
  ATTGAAGCCTGTGCCCAGGTCCCTGGAATGCAGGAGATTCTGCTCATTGGCTTCTACCAA
  CCTGATGAGCCCCTCACCCAGTTCCTAGAAGCCGCCCAGCAGGAGTTTAACCTTCCAGTC
  AGGTACCTGCAGGAATTTGCCCCCCTAGGCACAGGGGGTGGTCTTTACCATTTTCGAGAC
  CAGATCCTGGCTGGGAGCCCCGAGGCATTCTTCGTGCTCAATGCTGATGTCTGCTCCGAC
  TTCCCCTTGAGTGCTATGTTGGAAGCCCACCGACGCCAGCGTCACCCTTTCTTACTCCTT
  GGCACTACGGCTAACAGGACGCAATCCCTCAACTACGGCTGCATCGTTGAGAATCCACAG
  ACACACGAGGTATTGCACTATGTGGAGAAACCCAGCACATTTATCAGTGACATCATCAAC
  TGCGGCATCTACCTCTTTTCTCCTGAAGCCTTGAAGCCTCTTCGGGATGTCTTCCAGCGT
  AATCAGCAGGATGGGCAATTGGAGGACTCACCAGGCTTGTGGCCAGGGGCAGGTACCATC
  CGCCTAGAGCAGGATGTGTTTTCAGCCCTGGCAGGGCAGGGCCAGATATACGTGCATCTC
  ACTGATGGTATCTGGAGTCAGATCAAGTCCGCAGGTTCAGCCCTCTACGCCTCCCGCCTC
  TACCTGAGCCGATACCAGGACACTCACCCAGAACGGCTGGCCAAGCACACCCCAGGGGGC
  CCATGGATCCGAGGGAATGTGTACATCCACCCGACCGCCAAGGTGGCCCCCTCGGCTGTG
  CTGGGCCCCAACGTCTCCATCGGGAAGGGGGTGACCGTGGGTGAGGGTGTGCGGCTCCGG
  GAGAGCATCGTCCTCCATGGAGCCACTTTGCAGGAGCACACGTGTGTTCTGCATAGCATC
  GTGGGCTGGGGGAGCACCGTGGGACGCTGGGCGCGCGTGGAGGGTACCCCCAGTGACCCT
  AACCCCAACGATCCCCGAGCCCGCATGGACAGTGAGAGCCTCTTCAAGGACGGGAAGCTG
  CTGCCTGCTATCACCATCCTGGGCTGCCGAGTCCGGATCCCTGCCGAGGTGCTCATCCTG
  AACTCGATTGTTCTGCCACACAAGGAGCTGAGCCGAAGCTTCACCAACCAGATCATCCTC
  TGA
  
  Clone variation with respect to NM_013335.3
  1053 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013335 unedited
  CCGTTCAGAATTTGATATACGACTTTACTATAGGGCGGCCGCGAATTCGCACGAGGTGAA
  GGGAGCTATGTGTTTAGGTAGCAGTCACCATTATGCTCAAAGCGGTGATCCTGATTGGAG
  GCCCTCAAAAGGGAACTCGCTTCAGACCTTTGTCTTTTGAGGTGCCCAAACCATTGTTTC
  CTGTGGCAGGGGTCCCTATGATCCAACACCATATTGAAGCCTGTGCCCAGGTCCCTGGAA
  TGCAGGAGATTCTGCTCATTGGCTTCTACCAACCTGATGAGCCCCTCACCCAGTTCCTAG
  AAGCCGCCCAGCAGGAGTTTAACCTTCCAGTCAGGTACCTGCAGGAATTTGCCCCCCTAG
  GCACAGGGGGTGGTCTTTACCATTTTCGAGACCAGATCCTGGCTGGGAGCCCCGAGGCAT
  TCTTCGTGCTCAATGCTGATGTCTGCTCCGACTTCCCCTTGAGTGCTATGTTGGAAGCCC
  ACCGACGCCAGCGTCACCCTTTCTTACTCCTTGGCACTACGGCTAACAGGACGCAATCCC
  TCAACTACGGCTGCATCGTTGAGAATCCACAGACACACGAGGTATTGCACTATGTGGAGA
  AACCCAGCACATTTATCAGTGACATCATCAACTGCGGCATCTACCTCTTTTCTCCTGAAG
  CCTTTGAGCCTCTTCGGGATGTCTTCCAGCGTAATCAGCAGGATGGGCAATTGGAGGACC
  TCACCAGCTTGTGGCCCAGGGCAGGTACCATCCGCCTAGAGCAGGATGTGTTTTCAGCCC
  TGGCAGGCCAGGCCCAGATATACGTGCATCTCACTGATGGTATCCTGAAGTCAGATCAAG
  TCGCAAGTTCAGCCCTCTACGCCTNNCGCCTTTACCTGAGCCGATACCAGGACACTCACC
  CAGAACGGCTGGCCAAGCACACCCAGGGGGCCCATGAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013335 unedited
  GGGGGGCCATGNNNGATTCTTTTNNNNNNNGGNTTTTCGTGNACCCGGACCGCATTTTAN
  GATCGGTTTTTTTTTTTTTTTTTTTTCCAGGTTCACGGGGTTTATTAGGGAGTCGGGAGG
  GAGAAAACCCAGGAGTCCCCAGGCCATCCACATTGCTCCCCGGCATGTGACGATCCAGCC
  TGGCTTTCTCTGGTCCTTTCTGGACAGAGGCTGGCCAAGCAGGCAGCAGCCTCAAGGGGA
  GTGGGTAGGAGCTGGGGGCCTTCTGGCAGCCCTACTCAGAGGATGATCTGGTTGGTGAAG
  CTTCGGCTCAGCTCCTTGTGTGGCAGAACAATCGAGTTCAGGATGAGCACCTCGGCAGGG
  ATCCGGACTCGGCAGCCCAGGATGGTGATAGCAGGCAGCAGCTTCCCGTCCTTGAAGAGG
  CTCTCACTGTCCATGCGGGCTCGGGGATCGTTGGGGTTAGGGTCACTGGGGGTACCCTCC
  ACGCGCGCCCAGCGTCCCACGGTGCTCCCCCAGCCCACGATGCTATGCAGAACACACGTG
  TGCTCCTGCAAAGTGGCTCCATGGAGGACGATGCTCTCCCGGAGCCGCACACCCTCACCC
  ACGGTCACCCCCTTCCCGATGGAGACGTTGGGGCCCAGCACAGCCGAGGGGGCCACCTTG
  CCGGTCGGGTGGATGTACACATTCCCCTCGATCCATGGGCCCCCTGGGGTGTGCTTGGCC
  AGCCGTTCTGGGTGAGTGTCCTGGTATCCGCTCAGGTAAGGCGGGAGGCCTAAAAGCCTT
  GACCTGCGGACTGGATCTGACTTCAGAACCCTCATGGAAATGCCCGTTTTTTGGCCCTGC
  CCTGCCAGGGCTGAAAACAATTCTGCTCTAGGCCGATGGACCCTGCCCTGGCCAAAACCC
  GGGGAAGCCCTCAATGGCCATCTGCTGATACCCCTGAAAGACATCCGAAAAGCCTTAAGG
  TTTCAGGAAAAAAAAGTAATCCCCCATTGATGAGTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013335
• Insert Size: 1570 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013335.2, NP_037467.2
• RefSeq Size: 1543 bp
• RefSeq ORF: 1263 bp
• Locus ID: 29926
• UniProt ID: Q96IJ6
• Cytogenetics: 2q35
• Domains: hexapep, NTP_transferase
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: This gene is thought to encode a GDP-mannose pyrophosphorylase. This enzyme catalyzes the reaction which converts mannose-1-phosphate and GTP to GDP-mannose which is involved in the production of N-linked oligosaccharides. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) uses two different splice sites in the 5' UTR, compared to variant 2. Variants 1 and 2 encode the same protein.