Fibrinogen alpha chain (FGA) (NM_021871) Human Untagged Clone

CAT#: SC112555

FGA (untagged)-Human fibrinogen alpha chain (FGA), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Fibrinogen alpha chain
• Synonyms: Fib2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112555 sequence for NM_021871 edited (data generated by NextGen Sequencing)
  ATGTTTTCCATGAGGATCGTCTGCCTGGTCCTAAGTGTGGTGGGCACAGCATGGACTGCA
  GATAGTGGTGAAGGTGACTTTCTAGCTGAAGGAGGAGGCGTGCGTGGCCCAAGGGTTGTG
  GAAAGACATCAATCTGCCTGCAAAGATTCAGACTGGCCCTTCTGCTCTGATGAAGACTGG
  AACTACAAATGCCCTTCTGGCTGCAGGATGAAAGGGTTGATTGATGAAGTCAATCAAGAT
  TTTACAAACAGAATAAATAAGCTCAAAAATTCACTATTTGAATATCAGAAGAACAATAAG
  GATTCTCATTCGTTGACCACTAATATAATGGAAATTTTGAGAGGCGATTTTTCCTCAGCC
  AATAACCGTGATAATACCTACAACCGAGTGTCAGAGGATCTGAGAAGCAGAATTGAAGTC
  CTGAAGCGCAAAGTCATAGAAAAAGTACAGCATATCCAGCTTCTGCAGAAAAATGTTAGA
  GCTCAGTTGGTTGATATGAAACGACTGGAGGTGGACATTGATATTAAGATCCGATCTTGT
  CGAGGGTCATGCAGTAGGGCTTTAGCTCGTGAAGTAGATCTGAAGGACTATGAAGATCAG
  CAGAAGCAACTTGAACAGGTCATTGCCAAAGACTTACTTCCCTCTAGAGATAGGCAACAC
  TTACCACTGATAAAAATGAAACCAGTTCCAGACTTGGTTCCCGGAAATTTTAAGAGCCAG
  CTTCAGAAGGTACCCCCAGAGTGGAAGGCATTAACAGACATGCCGCAGATGAGAATGGAG
  TTAGAGAGACCTGGTGGAAATGAGATTACTCGAGGAGGCTCCACCTCTTATGGAACCGGA
  TCAGAGACGGAAAGCCCCAGGAACCCTAGCAGTGCTGGAAGCTGGAACTCTGGGAGCTCT
  GGACCTGGAAGTACTGGAAACCGAAACCCTGGGAGCTCTGGGACTGGAGGGACTGCAACC
  TGGAAACCTGGGAGCTCTGGACCTGGAAGTGCTGGAAGCTGGAACTCTGGGAGCTCTGGA
  ACTGGAAGTACTGGAAACCAAAACCCTGGGAGCCCTAGACCTGGTAGTACCGGAACCTGG
  AATCCTGGCAGCTCTGAACGCGGAAGTGCTGGGCACTGGACCTCTGAGAGCTCTGTATCT
  GGTAGTACTGGACAATGGCACTCTGAATCTGGAAGTTTTAGGCCAGATAGCCCAGGCTCT
  GGGAACGCGAGGCCTAACAACCCAGACTGGGGCACATTTGAAGAGGTGTCAGGAAATGTA
  AGTCCAGGGACAAGGAGAGAGTACCACACAGAAAAACTGGTCACTTCTAAAGGAGATAAA
  GAGCTCAGGACTGGTAAAGAGAAGGTCACCTCTGGTAGCACAACCACCACGCGTCGTTCA
  TGCTCTAAAACCGTTACTAAGACTGTTATTGGTCCTGATGGTCACAAAGAAGTTACCAAA
  GAAGTGGTGACCTCCGAAGATGGTTCTGACTGTCCCGAGGCAATGGATTTAGGCACATTG
  TCTGGCATAGGTACTCTGGATGGGTTCCGCCATAGGCACCCTGATGAAGCTGCCTTCTTC
  GACACTGCCTCAACTGGAAAAACATTCCCAGGTTTCTTCTCACCTATGTTAGGAGAGTTT
  GTCAGTGAGACTGAGTCTAGGGGCTCAGAATCTGGCATCTTCACAAATACAAAGGAATCC
  AGTTCTCATCACCCTGGGATAGCTGAATTCCCTTCCCGTGGTAAATCTTCAAGTTACAGC
  AAACAATTTACTAGTAGCACGAGTTACAACAGAGGAGACTCCACATTTGAAAGCAAGAGC
  TATAAAATGGCAGATGAGGCCGGAAGTGAAGCCGATCATGAAGGAACACATAGCACCAAG
  AGAGGCCATGCTAAATCTCGCCCTGTCAGAGGTATCCACACTTCTCCTTTGGGGAAGCCT
  TCCCTGTCCCCCTAG
  
  Clone variation with respect to NM_021871.2
  991 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021871 unedited
  GCACGAGGAGTGCTCCTCAGNAGCCAGCCCCACCCTTAGAAAAATGTTTTCCATTGAGGA
  TCGTCTGCCTGGTCCTAAGTGTGGTGGGCACAGCATGGACTGCAGATAGTGGTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021871 unedited
  NAATCTTTGNNACCGCGGCCGCAATCTAGNATCGAGNTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTCCATTAAACATGAATTTATTGAGTCATGGCTCTGTACTGTTAGGCATTTGG
  GAATACAGAGATGAAACAGACAAAGGCCCTGCCCCCAAGGAACTTACAGTCTAGCACAAA
  AACAGACCAAAAAAGGGTAGTTTCAATGACGTGTAACAGAGAGTTAAAAAGGAAATGCAG
  GGGGCCATGGGAACACTGGGCAAAAATATTTAACTTAGTCTAGGGGGACAGGGAAGGCTT
  CCCCAAAGGAAAAGGGGGGATACCTCTGACAGGGCGAGATTTACCATGGCCTCTCTTGGG
  GCTATGGGTTCCTTCATGATCGGCTTCACTTCCGGCCTCATCTGCCATTTTATAGCTCTT
  GCTTTCAAATGTGGAGTCTCCTCTGTTGAAACTCGGGCTACTAGTAAATTGTTTGCTGTA
  ACTTGAAGATTTACCACGGGAAGGGAATTCAGCTATCCCAGGGTGATGAGAACTGGATTC
  CTTTGTATTTGTGAAAATGCCAAATTCTGAGCCCCTAGACTCAGTCTCACTGACAAACTC
  TCCTAACATAGGTGAGAAGAAACCTGGGAATGTTTTTCCAGTTGAGGCAGTGTCAAAGAA
  GGCAGCTTCATCAGGGTGCCTATGGCGGAACCCATCCAGAGTACCTATGCCAGACAATGT
  GCCTAAATCCATTGCCTCGGGACAGTCAGAACCATCTTCGGAGGTCACCACTTCTTTGGT
  AACTTCTTTGNGACCATCAGGACCATTACAGTCTTAGTAACGGNTTTAGAGCATGAACGA
  CGCGTGGTGGGTTGGGCTACCAGAGGGTGACTTNCTCTTAACNAGTCCTGAGCTCTTTAT
  CTCTTTAGAAGTGACCAGTTTTNCTGTGNGGNACTCTCTCCTTGNCCCTGGAACTAACAT
  TNCTGACACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021871
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021871.2, NP_068657.1
• RefSeq Size: 2210 bp
• RefSeq ORF: 1935 bp
• Locus ID: 2243
• UniProt ID: P02671
• Cytogenetics: 4q31.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes the alpha subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, the encoded preproprotein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (alpha) is alternatively spliced in the 3' coding region and 3' UTR, resulting in a shorter isoform (alpha) with a different carboxy-terminus compared to isoform alpha-E. The encoded isoform (alpha) may undergo proteolytic processing similar to isoform alpha-E. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.