Prostaglandin E Synthase (PTGES) (NM_004878) Human Untagged Clone

CAT#: SC111734

PTGES (untagged)-Human prostaglandin E synthase (PTGES)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Prostaglandin E Synthase
• Synonyms: MGST-IV; MGST1-L1; MGST1L1; MPGES; mPGES-1; PGES; PIG12; PP102; PP1294; TP53I12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111734 sequence for NM_004878 edited (data generated by NextGen Sequencing)
  ATGCCTGCCCACAGCCTGGTGATGAGCAGCCCGGCCCTCCCGGCCTTCCTGCTCTGCAGC
  ACGCTGCTGGTCATCAAGATGTACGTGGTGGCCATCATCACGGGCCAAGTGAGGCTGCGG
  AAGAAGGCCTTTGCCAACCCCGAGGATGCCCTGAGACACGGAGGCCCCCAGTATTGCAGG
  AGCGACCCCGACGTGGAACGCTGCCTCAGGGCCCACCGGAACGACATGGAGACCATCTAC
  CCCTTCCTTTTCCTGGGCTTCGTCTACTCCTTTCTGGGTCCTAACCCTTTTGTCGCCTGG
  ATGCACTTCCTGGTCTTCCTCGTGGGCCGTGTGGCACACACCGTGGCCTACCTGGGGAAG
  CTGCGGGCACCCATCCGCTCCGTGACCTACACCCTGGCCCAGCTCCCCTGCGCCTCCATG
  GCTCTGCAGATCCTCTGGGAAGCGGCCCGCCACCTGTGA
  
  Clone variation with respect to NM_004878.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004878 unedited
  TATTTTGTAATACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGGTTGAGCTGCGC
  TGGCCAGAGATGCCTGCCCACAGCCTGGTGATGAGCAGCCCGGCCCTCCCGGCCTTCCTG
  CTCTGCAGCACGCTGCTGGTCATCAAGATGTACGTGGTGGCCATCATCACGGGCCAAGTG
  AGGCTGCGGAAGAAGGCCTTTGCCAACCCCGAGGATGCCCTGAGACACGGAGGCCCCCAG
  TATTGCAGGAGCGACCCCGACGTGGAACGCTGCCTCAGGGCCCACCGGAACGACATGGAG
  ACCATCTACCCCTTCCTTTTCCTGGGCTTCGTCTACTCCTTTCTGGGTCCTAACCCTTTT
  GCCGCCTGGATGCACTTCCTGGTCTTCCTCGTGGGCCGTGTGGCACACACCGTGGCCTAC
  CTGGGGAAGCTGCGGGCACCCATCCGCTCCGTGACCTACACCCTGGCCCAGCTCCCCTGC
  GCCTCCATGGCTCTGCAGATCCTCTGGGAAGCGGCCCGCCACCTGTGACCAGCAGCGGAT
  GCCTCCTTGGCCACCAGACCATGGGCCAAGAGCCGCCGTGGCTATACCTGGGGACTTGAT
  GTTCCTTCCAGATTGTGGTGGGCCCTGAGTCCTGGTTTCCTGGCAGCCTGCTGCGCGTGT
  GGGTCTCTGGGCACAGTGGGCCTGTGTGTGTGCCCGTGTGTGTGTATGTGTGTGTGTATG
  TTTCTTAGCCCCTTGGATTCCTGCACGAAGTGGCTGATGGGAACCATNNTCAGACAGATG
  TGAAGATTGATAGAAAATCCTTCAGCTAAAGTACAGAGCATCAAAACATCACTTCCTCTC
  CCTTCCCTACAGTGAAAGAGAGAGGNAGACTCTATNTAAGAATCCCAACCCTATGATCAT
  CTGAATCCCGGGGCTAGATGCAGACTTTCAGACTGACCCCANAAATCTGCCCCACCATCT
  AGAGGCAGCTGGCCTCTGNATTTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004878 unedited
  NNNNTTTTNNNNNNNNNNNNNNAAAAAAAAAAAAAAGAATTGTCACTTACCTTTNATGGA
  CTAAAAACACACANCTNCAGGTNCACGGGTCTAGGNANAAACACACACACACACACACAC
  ACACACACACACACACACACACACGGATTCCCCATCAAGGGGACATTTGCAGTTTCCAAA
  CCTTGAAGATACTGAAGGGACCAGAAAGTTCCTTTGAGTGGCTGGTCACCCAAAGCTCCC
  GGTCCTCCACCCACTGCCCTTTGGAGGGACTCAAACCTTGGGAGGAGAAGGCTGAGCTTC
  CTGTGGGCCCCTCCCACCCACACCTGAGCCAGAGAGAAGACTGCAGCAAAGACATCCAAA
  GCCAACGGCAAGGGAAGCGTCAGCGGGGGCAGAGGAGCCAGCCCTGTCCTTGGCTCACCC
  AGCTTCCACCATACAGGAACCCAAGACCCCAGCCTTGCTTCCACAGAGAACTGGCAGGGG
  TCCCCTGGCCTGGCCATCACAGGGACTCACATGGGAGCCTTTTAAAACTCCAGATGGTGG
  CTGAGCACAGTGATTCATGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGCCGGTGGATC
  ACTTGAGGCCAGGAGTTCGAGACCCTCCTGGGCAACATGGTGAACCCGTCTCTACTAAAA
  ATACAAAAATTAGCTGGGTATGGTGATACGCGCCTGTAATCCCAGCTACTCAGGAGGCTG
  AGGCGGGAGAATCGTTTGAACCCGGGAGCGGAGGCTGCAGTGAGCCATATTGTACCACTT
  CACTCCAGTTTGGGCAACAGAGCAAGACTCTGTTTTGAAAAAAAAAATACAGTGGCCAGG
  CTTGCCTTTAGATGGCTGGGGCAAAATTTTTGGGGTCATCTAAAAGTCTGCATTTTTACC
  CCGGGATTAAAAGACCATTAGTTTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004878
• Insert Size: 1860 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004878.3, NP_004869.1
• RefSeq Size: 1805 bp
• RefSeq ORF: 459 bp
• Locus ID: 9536
• UniProt ID: O14684
• Cytogenetics: 9q34.11
• Domains: MAPEG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a glutathione-dependent prostaglandin E synthase. The expression of this gene has been shown to be induced by proinflammatory cytokine interleukin 1 beta (IL1B). Its expression can also be induced by tumor suppressor protein TP53, and may be involved in TP53 induced apoptosis. Knockout studies in mice suggest that this gene may contribute to the pathogenesis of collagen-induced arthritis and mediate acute pain during inflammatory responses. [provided by RefSeq, Jul 2008]