Tapasin (TAPBP) (NM_003190) Human Untagged Clone

CAT#: SC111697

TAPBP (untagged)-Human TAP binding protein (tapasin) (TAPBP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Tapasin
• Synonyms: NGS17; TAPA; TPN; TPSN
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003190, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGTCCCTGTCTCTGCTCCTCGCTGTGGCTTTGGGCCTGGCGACCGCCGTCTCAGCAGGACCCGCGG
  TGATCGAGTGTTGGTTCGTGGAGGATGCGAGCGGAAAGGGCCTGGCCAAGAGACCCGGTGCACTGCTGTT
  GCGCCAGGGACCGGGGGAACCGCCGCCCCGGCCGGACCTCGACCCTGAGCTCTATCTCAGTGTACACGAC
  CCCGCGGGCGCCCTCCAGGCTGCCTTCAGGCGGTATCCCCGGGGCGCCCCCGCACCACACTGCGAGATGA
  GCCGCTTCGTGCCTCTCCCCGCCTCTGCGAAATGGGCCAGCGGCCTGACCCCCGCGCAGAACTGCCCGCG
  GGCCCTGGATGGGGCTTGGCTGATGGTCAGCATATCCAGCCCAGTCCTCAGCCTCTCCAGCCTCTTGCGA
  CCACAGCCAGAGCCTCAGCAGGAGCCTGTTCTCATCACCATGGCAACAGTGGTACTGACTGTCCTCACCC
  ACACCCCTGCCCCTCGAGTGAGACTGGGACAAGATGCTCTGCTGGACTTGAGCTTTGCCTACATGCCCCC
  CACCTCCGAGGCCGCCTCATCTCTGGCTCCGGGTCCCCCTCCCTTTGGGCTAGAGTGGCGACGCCAGCAC
  CTGGGTAAGGGACATCTGCTCCTGGCTGCAACTCCTGGGCTGAATGGCCAGATGCCAGCAGCCCAAGAAG
  GGGCCGTGGCATTTGCTGCTTGGGATGATGATGAGCCATGGGGCCCATGGACCGGAAATGGGACCTTCTG
  GCTGCCTACAGTTCAACCCTTTCAGGAGGGCACCTATCTGGCCACCATACACCTGCCATACCTGCAAGGA
  CAGGTCACCCTGGAGCTTGCTGTGTACAAACCCCCCAAAGTGTCCCTGATGCCAGCAACCCTTGCACGGG
  CCGCCCCAGGGGAGGCACCCCCGGAATTGCTCTGCCTTGTGTCCCACTTCTACCCTTCTGGGGGCCTGGA
  GGTGGAGTGGGAACTCCGGGGTGGCCCAGGGGGCCGCTCTCAGAAGGCCGAGGGGCAGAGGTGGCTCTCG
  GCCCTGCGCCACCATTCCGATGGCTCTGTCAGCCTCTCTGGGCACTTGCAGCCGCCCCCAGTCACCACTG
  AGCAGCATGGGGCACGCTATGCCTGTCGAATTCACCATCCCAGCCTGCCTGCCTCGGGGCGCAGCGCTGA
  GGTCACCCTGGAGGTAGCAGGTCTTTCAGGGCCCTCCCTTGAGGACAGCGTAGGCCTTTTCCTGTCTGCC
  TTTCTTCTGCTTGGGCTCTTCAAGGCACTGGGCTGGGCTGCTGTCTACCTGTCCACCTGCAAGGATTCAA
  AGAAGAAAGCAGAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003190 unedited
  ATCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA
  GAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAA
  TTCGGCACCAGGCAGCGCCATGAAGTCCCTGTCTCTGCTCCTCGCTGTGGCTTTGGGCCT
  GGCGACCGCCGTCTCAGCAGGACCCGCGGTGATCGAGTGTTGGTTCGTGGAGGATGCGAG
  CGGAAAGGGCCTGGCCAAGAGACCCGGTGCACTGCTGTTGCGCCAGGGACCGGGGGAACC
  GCCGCCCCGGCCGGACCTCGACCCTGAGCTCTATCTCAGTGTACACGACCCCGCGGGCGC
  CCTCCAGGCTGCCTTCAGGCGGTATCCCCGGGGCGCCCCCGCACCACACTGCGAGATGAG
  CCGCTTCGTGCCTCTCCCCGCCTCTGCGAAATGGGCCAGCGGCCTGACCCCCGCGCAGAA
  CTGCCCGCGGGCCCTGGATGGGGCTTGGCTGATGGTCAGCATATCCAGCCCAGTCCTCAG
  CCTCTCCAGCCTCTTGCGACCACAGCCAGAGCCTCAGCAGGAGCCTGTTCTCATCACCAT
  GGCAACAGTGGTACTGACTGTCCTCACCCACACCCCTGCCCCTCGAGTGAGACTGGGACA
  AGATGCTCTGCTGGACTTGAGCTTTGCCTACATGCCCCCCACCTCCGAAGCCGCCTCATC
  TCTGGCTCCGGGTCCCCCCTCCTTTGGGCTAGAGTGGCGACGCCAGCACCTGGGTAAGGG
  ACATCTGCTCCTGGNCTGCACTCCTGGGCTGAATGGCCAGATGCCAGCAGCCCAAGAGGG
  GCCGTGGGCATTGCTGCTTGGGATGATGATGAGCCATGGGGCCCATGGGACCGGGAATGG
  GACCNTCTGGCTGNCTAGAGTTCAACCCTTTCAGGAGGGNCACTATCTGCCACCATACAC
  C
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003190 unedited
  TAAAAGCGCTACTATGTACGCGGCCGCTTTTTANNGTCGAGTTTTTTTTTTTTTTTTTTT
  TTAGGAGCAGATGATAGGGTATTATTTTAGGGAGCTACTACAGAAGCTTGGGCCAGAGAT
  GATGGTGGCTTCCACAGGATGGCAGTGAGTGCCCTCACTCTGCTTTCTTCTTTGAATCCT
  TGCAGGTGGACAGGTAGACAGCAGCCCAGCCCAGTGCCTTGAAGAGCCCAAGCAGAAGAA
  AGGCAGACAGGAAAAGGCCTACGCTGTCCTCAAGGGAGGGCCCTGAAAGACCTGCTACCT
  CCAGGGTGACCTCAGCGCTGCGCCCCGAGGCAGGCAGGCTGGGATGGTGAATTCGACAGG
  CATAGCGTGCCCCATGCTGCTCAGTGGTGACTGGGGGCGGCTGCAAGTGCCCAGAGAGGC
  TGACAGAGCCATCGGAATGGTGGCGCAGGGCCGAGAGCCACCTCTGCCCCTCGGCCTTCT
  GAGAGCGGCCCCCTGGGCCACCCCGGAGTTCCCACTCCACCTCCAGGCCCCCAGAATGGT
  AGAAGTGGGACACAAAGCAGAGCAATTCCGGGGGTGCCTCCCCTGGGGCGGCCCGTGCAA
  GGGTTGCTGGCATCAAGGACACTTTGGGGGGTTTGTACACAGCAAGCTCCAGGTGACCTG
  TCCTTGCAGTTTGGCAGGTGTTTGGTGGCCANATAGGTGCCCTCCTGAAGGGTTGAAACT
  CTAGGCAGCCAGAAAAGTCCCATTTTCGGGGCATTGGGCCCCATGGCTCATCTTTCTCCC
  AAGCAGGCAAATGCCCCGGGCCCCTTTCTTGGGCTGCTGGGCATTTGGGCCTTTCAAGCC
  CAGGAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003190
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003190.3, NP_003181.3
• RefSeq Size: 3629 bp
• RefSeq ORF: 1347 bp
• Locus ID: 6892
• UniProt ID: O15533
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Antigen processing and presentation
• Gene Summary: This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.