POLR3H (NM_138338) Human Untagged Clone

CAT#: SC111545

POLR3H (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) (POLR3H), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR3H
• Synonyms: C25; RPC8; RPC22.9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111545 sequence for NM_138338 edited (data generated by NextGen Sequencing)
  ATGTTCGTCCTGGTGGAAATGGTGGACACCGTCCGGATCCCCCCTTGGCAGTTTGAGAGG
  AAGCTCAACGACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCGTGTAC
  AACGTGGGACTCTGCATTTGTCTGTTTGATATCACCAAACTGGAGGATGCCTATGTATTC
  CCTGGGGATGGCGCATCACACACCAAAGTCCATTTTCGCTGCGTGGTGTTTCATCCATTC
  CTAGATGAGATTCTCATTGGGAAGATCAAAGGCTGCAGCCCAGAAGGAGTGCACGTCTCT
  CTAGGCTTCTTCGATGACATTCTCATCCCCCCAGAGTCACTGCAGCAGCCAGCCAAGTTC
  GACGAAGCGGAGCAGGTGTGGGTGTGGGAGTACGAGACGGAGGAAGGAGCACACGACCTC
  TACATGGACACCGGCGAGGAGATCCGCTTCCGGGTGGTGGACGAGAGCTTTGTTGACACG
  TCCCCCACAGGGCCCAGCTCAGCAGATGCCACCACTTCCAGTGAGGAGCTGCCAAAGAAG
  GAGGCTCCGTACACGCTTGTGGGATCCATCAGTGAGCCAGGCCTGGGCCTTCTCTCCTGG
  TGGACCAGCAACTAG
  
  Clone variation with respect to NM_138338.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138338 unedited
  GTCCGAATATGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGCGGGGT
  CTCGCGGCGCGGGCGCGCACCCGNGACTGTGGACGGAGAGTGCCTCCCTCTGGCCTCAGT
  TTCCTCATGTTGTAGTAGCGGAGCATGGCCCGGACCGGGCCCCCGAGACCGCCCCGTGCA
  ACCTCACCGCCAGCCTGGGGGCCTCAGCGACTGGGACGGGACCAAGGGGCTCGGGGATTC
  TCCCTGCCCCCGGCCCTGGTGCGTGACTGACCCTCCTGTTCCCAGAGCCCCCAGCGCAGG
  CCGGGATGTTCGTCCTGGTGGAAATGGTGGACACCGTCCGGATCCCCCCTTGGCAGTTTG
  AGAGGAAGCTCAACGACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCG
  TGTACAACGTGGGACTCTGCATTTGTCTGTTTGATATCACCAAACTGGAGGATGCCTATG
  TATTCCCTGGGGATGGCGCATCACACACCAAAGTCCATTTTCGCTGCGTGGTGTTTCATC
  CATTCCTAGATGAGATTCTCATTGGGAAGATCAAAGGCTGCAGCCCAGAAGGAGTGCACG
  TCTCTCTAGGCTTCTTCGATGACATTCTCATCCCCCCAGAGTCACTGCAGCAGCCAGCCA
  AGTTCGACGAAGCGGAGCAAGTGTGGGTGTGGGAGTACCAGACGGAGGAAGGAGCACACG
  ACCTCTACATGGACACCGGCGAGGAGATCCGCTTCCGGGTGGTGGACGAGAGCTTTGNTG
  ACACGTNCCCCACAGGNCCCAGCTCAGCAGATGCCACCACTTNCAGTGANGAGCTGCCAA
  AGAAAGAGCTNCGTACACGCTTTGTGGATCCATCAGTGAGCCAGGCCCTGGCCTTCTCTT
  CTGGTGGACCCAGCACTAGCCCTGGNGGCTGNAAGTGAACCCTACCACCTGCGGNAANGG
  TGTATGNCNGCATGGAAGACACGCAGCTGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138338 unedited
  GGGTTTTATTCTGTTTCAGGCTCCCTCTAAGTTTTTCTCCTGCCTGATCATTTCATTGGT
  GGCTGAAGGATTCTAGAGAACCTTTTGTTCTTGCAAGGAAAACAAGAATCCAAAACCAGT
  GACTGTTCTGTGAGTGATTGGTGTCTGTGCCGTTTGTTGTCAAGTCCAGGGTCCAGGAAG
  GGTCTTTTCAGTCAAGGTCAGTGTTTTCCCAACAGCCACAGCCACAGATGGATCCATCAC
  TGCAGTGAAGGAGGAGCAGGGCCTAGATGGTGGAGGAGCGGGGCAAGCTGGTGGGCACTC
  CTGGCCTTGCCTCACTGTCCAGCTCGAGACACTATCTCCTTAGCATCGGCCTCAGCTGCT
  GTTGTCTTCACAGCCGGCCATACCACCTTCCCGCAGGCTGGTAGGGTCCACTGTCCAGCC
  CCAGGGCTAGTTGCTGGTCCACCAGGAGAGAAGGCCCAGGCCTGGCTCACTGATGGATCC
  CACAAGCGTGTACGGAGCCTCCTTCTTTGGCAGCTCCTCACTGGAAGTGGTGGCATCTGC
  TGAGCTGGGCCCTGTGGGGGACGTGTCAACAAAGCTCTCGTCCACCACCCGGAAGCGGAT
  CTCCTCGCCGGTGTCCATGTAGAGGTCGTGTGCTCCTTCCTCCGTCTCGTACTCCCACAC
  CCACACCTGGCTCGCTTCGTCGAACTTGGCTGGCTGCTGCAGTGACTCTGGGGGGATGAG
  AATGTCATCGAAAGAGCCTAGAGAGACGTGCACTCCTTCTGGGCTGCAGCCTTTGATCCT
  TCCNATGAGGATCTCAATCTAGGAATGGATGAAACCCACGCCAGCGAAAATGGACTTTGG
  TGTGTGAGCGCCATCCCCAGGGATACATGGCCATCCTCAGTTTTGGGGAATCAAACAACA
  AATGCGCAGTCCACGTTTTACCGAACTTGGTGGGCACCTTTCTGGTCACCTCCTCGGCAT
  GGG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_138338
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138338.2, NP_612211.1
• RefSeq Size: 4159 bp
• RefSeq ORF: 615 bp
• Locus ID: 171568
• UniProt ID: Q9Y535
• Cytogenetics: 22q13.2
• Domains: RNA_pol_Rpb7_N
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 3, 5, and 6 encode the same isoform (a).