TBK1 (NM_013254) Human Untagged Clone

CAT#: SC111251

TBK1 (untagged)-Human TANK-binding kinase 1 (TBK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBK1
• Synonyms: FTDALS4; IIAE8; NAK; T2K
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_013254 edited
  ATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTTAGGCCAAGGAGCTACT
  GCAAATGTCTTTCGTGGAAGACATAAGAAAACTGGTGATTTATTTGCTATCAAAGTATTT
  AATAACATAAGCTTCCTTCGTCCAGTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAA
  AAACTCAATCACAAAAATATTGTCAAATTATTTGCTATTGAAGAGGAGACAACAACAAGA
  CATAAAGTACTTATTATGGAATTTTGTCCATGTGGGAGTTTATACACTGTTTTAGAAGAA
  CCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGTTTTGCGAGATGTGGTG
  GGTGGAATGAATCATCTACGAGAGAATGGTATAGTGCACCGTGATATCAAGCCAGGAAAT
  ATCATGCGTGTTATAGGGGAAGATGGACAGTCTGTGTACAAACTCACAGATTTTGGTGCA
  GCTAGAGAATTAGAAGATGATGAGCAGTTTGTTTCTCTGTATGGCACAGAAGAATATTTG
  CACCCTGATATGTATGAGAGAGCAGTGCTAAGAAAAGATCATCAGAAGAAATATGGAGCA
  ACAGTTGATCTTTGGAGCATTGGGGTAACATTTTACCATGCAGCTACTGGATCACTGCCA
  TTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAGAAGTGATGTATAAAATAATTACAGGA
  AAGCCTTCTGGTGCAATATCTGGAGTACAGAAAGCAGAAAATGGACCAATTGACTGGAGT
  GGAGACATGCCTGTTTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTT
  CTTGCAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTTTTTGCA
  GAAACTAGTGATATACTTCACCGAATGGTAATTCATGTTTTTTCGCTACAACAAATGACA
  GCTCATAAGATTTATATTCATAGCTATAATACTGCTACTATATTTCATGAACTGGTATAT
  AAACAAACCAAAATTATTTCTTCAAATCAAGAACTTATCTACGAAGGGCGACGCTTAGTC
  TTAGAACCTGGAAGGCTGGCACAACATTTCCCTAAAACTACTGAGGAAAACCCTATATTT
  GTAGTAAGCCGGGAACCTCTGAATACCATAGGATTAATATATGAAAAAATTTCCCTCCCT
  AAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGCTAAGGCAATAACAGGG
  GTTGTGTGTTATGCCTGCAGAATTGCCAGTACCTTACTGCTTTATCAGGAATTAATGCGA
  AAGGGGATACGATGGCTGATTGAATTAATTAAAGATGATTACAATGAAACTGTTCACAAA
  AAGACAGAAGTTGTGATCACATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAA
  GTATATGAAAAGTTGATGAAGATCAACCTGGAAGCGGCAGAGTTAGGTGAAATTTCAGAC
  ATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAACAATAGAAACCAGTCTTCAG
  GATATCGACAGCAGATTATCTCCAGGTGGATCACTGGCAGACGCATGGGCACATCAAGAA
  GGCACTCATCCGAAAGACAGAAATGTAGAAAAACTACAAGTCCTGTTAAATTGCATGACA
  GAGATTTACTATCAGTTCAAAAAAGACAAAGCAGAACGTAGATTAGCTTATAATGAAGAA
  CAAATCCACAAATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATGACGCAC
  TTTACAGATGAATGTGTTAAAAAGTATGAGGCATTTTTGAATAAGTCAGAAGAATGGATA
  AGAAAGATGCTTCATCTTAGGAAACAGTTATTATCGCTGACTAATCAGTGTTTTGATATT
  GAAGAAGAAGTATCAAAATATCAAGAATATACTAATGAGTTACAAGAAACTCTGCCTCAG
  AAAATGTTTACAGCTTCCAGTGGAATCAAACATACCATGACCCCAATTTATCCAAGTTCT
  AACACATTAGTAGAAATGACTCTTGGTATGAAGAAATTAAAGGAAGAGATGGAAGGGGTG
  GTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGGCTCTTTAACCATGGAT
  GGTGGCCTTCGCAACGTTGACTGTCTTTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013254 unedited
  ATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCCTCGTGCCGAA
  TTCGGCACGAGGCCCGCCGGCGGTGGCGCGGCGGAGACCCGGCTGGTATAACAAGAGGAT
  TGCCTGATCCAGCCAAGATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTT
  TAGGCCAAGGAGCTACTGCAAATGTCTTTCGTGGAAGACATAAGAAAACTGGTGATTTAT
  TTGCTATCAAAGTATTTAATAACATAAGCTTCCTTCGTCCAGTGGATGTTCAAATGAGAG
  AATTTGAAGTGTTGAAAAAACTCAATCACAAAAATATTGTCAAATTATTTGCTATTGAAG
  AGGAGACAACAACAAGACATAAAGTACTTATTATGGAATTTTGTCCATGTGGGAGTTTAT
  ACACTGTTTTAGAAGAACCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTG
  TTTTGCGAGATGTGGTGGGTGGAATGAATCATCTACGAGAGAATGGTATAGCGCACCGTG
  ATATCAAGCCAGGAAATATCATGCGTGTTATAGGGGAAGATGGACAGTCTGTGTACAAAC
  TCACAGATTTTGGTGCAGCTAGAGAATTACAAGATGATGAGCAGTTTGTTTCTCTGTATG
  GCACAGAAGAATATTTGCACCCTGATATGTATGAGAGAGCAGTGCTAAGAAAGATCATCA
  GAAGAATATGGAGCACCAGTTGATCTTTGGAGCATTGGGGTAACATTTTACCATGCAGCT
  ACTGCATCACTGCCATTTACACCCTTTGGAGGGCCTCGTAGGAATAAAGAAGTGATGTTT
  AAAATATTTACAGAAAAGCCCTTTGGTGCAAAATCTGAAGACCAGAAGCATAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013254 unedited
  CCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTTTACATTAAAATTGAGAA
  ATTTATTTTTTTCCATGAAATAGTAAGCAGAATTAAAGAAATATACAGCTTAAAACAGTT
  GAAATGACCTTAGAATTTTAATGACAATTACACATTTATGGCCAAAGGAAAAAAATGAAT
  AGTTTTTAATAATGAATTCACTTTAAAATGTTTGCTTAATAATTCATCTGAAATTACATG
  GATACAAGGATAACTGGGATCTGGGCACCTTGTAAAATAAAATAATGCCACTTTATCCTT
  CTCAAAAAAATTGTTATCCCAGTATAACAGTGGTGCGGTTCTGCTCCCCACTCACAGTAG
  CCACCGTTCTACCATTTACAAACGTGTCAAGTCAGTCTTCCTGATTTGTAAGGATGCCTA
  TTTTGGTTCCACTTAAAAATATTTCCAGTAGGCGGTCAGAGAAAATAACTTATTTAGCCC
  CACTCAGAGTAGAGGATCAGTATGTCAACATATTGGTCGGTCATGGCTTTCTTCTCGTGA
  TCACTGAGGTCAAATTTCCTCATATAATTATAAATGTTAAAAGATAAAATTACATCTTCA
  CAGCAGCCAAAAATTTGNATATTTTTTATGTATTTACAATATTGTACATATTTACACGAC
  CACATCAAATACTGAATTGTAGAAACAAGTGACTATCTATAAAGGCATTCATTTAATGCC
  CAAGCGTTATTTTCTTGTGCAAACGGAAACTTTTCTTAAACTTCTATTAAAAAGCTAAGA
  CAGTCAACGTTGCGAAGGCCACATCCATGGTTAAAGAGCCAAACCCTTTCTAAATGTGTT
  ATTTTCAGCAAGTTCTTTAACCACCCTTCCATCTCTTTCTTTAATTNCTCATACCAAGAT
  CCATTNTACTAAGTGGTAAAACTTGATAATGGGGGCATGGTATGTTGATCCCTGGAACTG
  AACATTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013254
• Insert Size: 2880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013254.2, NP_037386.1
• RefSeq Size: 2982 bp
• RefSeq ORF: 2190 bp
• Locus ID: 29110
• UniProt ID: Q9UHD2
• Cytogenetics: 12q14.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]