NFH (NEFH) (NM_021076) Human Untagged Clone

CAT#: SC110961

NEFH (untagged)-Human neurofilament, heavy polypeptide (NEFH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFH
• Synonyms: CMT2CC; NFH
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110961 sequence for NM_021076 edited (data generated by NextGen Sequencing)
  ATGATGAGCTTCGGCGGCGCGGACGCGCTGCTGGGCGCCCCGTTCGCGCCGCTGCATGGC
  GGCGGCAGCCTCCACTACGCGCTAGCCCGAAAGGGTGGCGCAGGCGGGACGCGCTCCGCC
  GCTGGCTCCTCCAGCGGCTTCCACTCGTGGACACGGACGTCCGTGAGCTCCGTGTCCGCC
  TCGCCCAGCCGCTTCCGTGGCGCAGGCGCCGCCTCAAGCACCGACTCGCTGGACACGCTG
  AGCAACGGGCCGGAGGGCTGCATGGTGGCGGTGGCCACCTCACGCAGTGAGAAGGAGCAG
  CTGCAGGCGCTGAACGACCGCTTCGCCGGGTACATCGACAAGGTGCGGCAGCTGGAGGCG
  CACAACCGCAGCCTGGAGGGCGAGGCTGCGGCGCTGCGGCAGCAGCAGGCGGGCCGCTCC
  GCTATGGGCGAGCTGTACGAGCGCGAGGTCCGCGAGATGCGCGGCGCGGTGCTGCGCCTG
  GGCGCGGCGCGCGGTCAGCTACGCCTGGAGCAGGAGCACCTGCTCGAGGACATCGCGCAC
  GTGCGCCAGCGCCTAGACGACGAGGCCCGGCAGCGAGAGGAGGCCGAGGCGGCGGCCCGC
  GCGCTGGCGCGCTTCGCGCAGGAGGCCGAGGCGGCGCGCGTGGACCTGCAGAAGAAGGCG
  CAGGCGCTGCAGGAGGAGTGCGGCTACCTGCGGCGCCACCACCAGGAAGAGGTGGGCGAG
  CTGCTCGGCCAGATCCAGGGCTCCGGCGCCGCGCAGGCGCAGATGCAGGCCGAGACGCGC
  GACGCCCTGAAGTGCGACGTGACGTCGGCGCTGCGCGAGATTCGCGCGCAGCTTGAAGGC
  CACGCGGTGCAGAGCACGCTGCAGTCCGAGGAGTGGTTCCGAGTGAGGCTGGACCGACTG
  TCGGAGGCAGCCAAGGTGAACACAGACGCTATGCGCTCAGCGCAGGAGGAGATAACTGAG
  TACCGGCGTCAGCTGCAGGCCAGGACCACAGAGCTGGAGGCACTGAAAAGCACCAAGGAC
  TCACTGGAGAGGCAGCGCTCTGAGCTGGAGGACCGTCATCAGGCCGACATTGCCTCCTAC
  CAGGAAGCCATTCAGCAGCTGGACGCTGAGCTGAGGAACACCAAGTGGGAGATGGCCGCC
  CAGCTGCGAGAATACCAGGACCTGCTCAATGTCAAGATGGCTCTGGATATAGAGATAGCC
  GCTTACAGAAAACTCCTGGAAGGTGAAGAGTGTCGGATTGGCTTTGGCCCAATTCCTTTC
  TCGCTTCCAGAAGGACTCCCCAAAATTCCCTCTGTGTCCACTCACATAAAGGTGAAAAGC
  GAAGAGAAGATCAAAGTGGTGGAGAAGTCTGAGAAAGAAACTGTGATTGTGGAGGAACAG
  ACAGAGGAGACCCAAGTGACTGAAGAAGTGACTGAAGAAGAGGAGAAAGAGGCCAAAGAG
  GAGGAGGGCAAGGAGGAAGAAGGGGGTGAAGAAGAGGAGGCAGAAGGGGGAGAAGAAGAA
  ACAAAGTCTCCCCCAGCAGAAGAGGCTGCATCCCCAGAGAAGGAAGCCAAGTCACCAGTA
  AAGGAAGAGGCAAAGTCACCGGCTGAGGCCAAGTCCCCAGAGAAGGAGGAAGCAAAATCC
  CCAGCCGAAGTCAAGTCCCCTGAGAAGGCCAAGTCTCCAGCAAAGGAAGAGGCAAAGTCA
  CCGCCTGAGGCCAAGTCCCCAGAGAAGGAGGAAGCAAAATCTCCAGCTGAGGTCAAGTCC
  CCCGAGAAGGCCAAGTCCCCAGCAAAGGAAGAGGCAAAGTCACCGGCTGAGGCCAAGTCT
  CCAGAGAAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGTCACCGGCTGAGGCCAAGTCC
  CCAGTGAAGGAAGAAGCAAAATCTCCAGCTGAGGTCAAGTCCCCGGAAAAGGCCAAGTCT
  CCAACGAAGGAGGAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGAGAAGGAAGAGGCC
  AAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGCAGAAGCAAAGTCCCCTGAGAAGGCC
  AAGTCCCCAGTGAAGGCAGAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGAA
  GAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGTCCCCTGAG
  AAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGACCCCCGAGAAGGCCAAGTCCCCAGTG
  AAGGAAGAAGCCAAGTCCCCAGAGAAGGCCAAGTCCCCAGAGAAGGCCAAGACTCTTGAT
  GTGAAGTCTCCAGAAGCCAAGACTCCAGCGAAGGAGGAAGCAAGGTCCCCTGCAGACAAA
  TTCCCTGAAAAGGCCAAAAGCCCTGTCAAGGAGGAGGTCAAGTCCCCAGAGAAGGCGAAA
  TCTCCCCTGAAGGAGGATGCCAAGGCCCCTGAGAAGGAGATCCCAAAAAAGGAAGAGGTG
  AAGTCCCCAGTGAAGGAGGAGGAGAAGCCCCAGGAGGTGAAAGTCAAAGAGCCCCCAAAG
  AAGGCAGAGGAAGAGAAAGCCCCTGCCACACCAAAAACAGAGGAGAAGAAGGACAGCAAG
  AAAGAGGAGGCACCCAAGAAGGAGGCTCCAAAGCCCAAGGTGGAGGAGAAGAAGGAACCT
  GCTGTCGAAAAGCCCAAAGAATCCAAAGTTGAAGCCAAGAAGGAAGAGGCTGAAGATAAG
  AAAAAAGTCCCCACCCCAGAGAAGGAGGCTCCTGCCAAGGTGGAGGTGAAGGAAGACGCT
  AAACCCAAAGAAAAGACAGAGGTGGCCAAGAAGGAACCAGATGATGCCAAGGCCAAGGAA
  CCCAGCAAACCAGCAGAGAAGAAGGAGGCAGCACCGGAGAAAAAAGACACCAAGGAGGAG
  AAGGCCAAGAAGCCTGAGGAGAAACCCAAGACAGAGGCCAAAGCCAAGGAAGATGACAAG
  ACCCTCTCAAAAGAGCCTAGCAAGCCTAAGGCAGAAAAGGCTGAAAAATCCTCCAGCACA
  GACCAAAAAGACAGCAAGCCTCCAGAGAAGGCCACAGAAGACAAGGCCGCCAAGGGGAAG
  TAA
  
  Clone variation with respect to NM_021076.3
  2232 t=>c;2784 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021076 unedited
  NGGTCATAATTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGCCGTCCCGGC
  CTCGCGCACCTGCTCAGGCCATGATGAGCTTCGGCGGCGCGGACGCGCTGCTGGGCGCCC
  CGTTCGCGCCGCTGCATGGCGGCGGCAGCCTCCACTACGCGCTAGCCCGAAGGGTGGCGC
  AGGCGGGACGCGCTCCGCCGCTGGCTCCTCCAGCGGCTTCCACTCGTGGACACGGACGTC
  CGTGAGCTCCGTGTCCGCCTCGCCCAGCCGCTTCCGTGGCGCAGGCGCCGCCTCAAGCAC
  CGACTCGCTGGACACGCTGAGCAACGGGCCGGAGGGCTGCATGGTGGCGGTGGCCACCTC
  ACGCAGTGAGAAGGAGCAGCTGCAGGCGCTGAACGACCGCTTCGCCGGGTACATCGACAA
  GGTGCGGCAGCTGGAGGCGCACAACCGCAGCCTGGAGGGCGAGGCTGCGGCGCTGCGGCA
  GCAGCAGGCGGGCCGCTCCGCTATGGGCGAGCTGTACGAGCGCGAGGTCCGCGAGATGCG
  CGGCGCGGTGCTGCGCCTGGGCGCGGCGCGCGGTCAGCTACGCCTGGAGCAGGAGCACCT
  GCTCGAGGACATCGCGCACGTGCGCCAGCGCCTAGACGACGAGGCCCGGCAGCGAGAGGA
  GGCCGAGGCGGCGGCCCGCGCGCTGGCGCGCTTCGCGCANGAGGCCGAGGCGGCGCGCGT
  GGACCTGCAGAAGAAGCGCAGGCGCTGCAGGAGGAGTGCGGCTACCTGCGGCGCCACCAC
  CAGGAAGAGGTGGGCGAGCTGCTCGGCCAGATCCAGGGCTCCGGCGCCGCGCCAGCGCAG
  ATGCANGCCGAGACGCGCGACGCCCTGAAGTGCGACGTGACGTCCGCGCTGCGCGAGATC
  C
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021076 unedited
  GTACCGCGGCACGCAATCTAGTAGTCGAGNNNNNNTTTTTTTTTTTTTTTTTTTCCATTT
  TATAAGCACTTGGTTTTATTGCACAGATACAATTGAAAGTGAACTCCAGTGGAAAGTGCG
  CCCTGGCATAATTCATTGGCCAGTGCCCGGCCCCACCTGGACCGCTCTTCCAAAATAAAG
  AGCTCTGGGCAAGGGCTTGACCACTCCTTTGGAAAACAGAAGGCATTTGGGAATAGGTTA
  AGACATCTCAAGCATAATGTATTTCTAACTGCTCATTCATTATTGCGGTCATCTGTCAGT
  TGCACATAGAGGCAATAAATGCACTTATTTGGAAAGGAGGGGGGCAGTTTTTATAGTCAA
  GTGTTTACGTGTGGCATTCGGCATGTATCACATTCAGCTACATAAGCTATCATAATTGTC
  CATCGCCTGGGGAGGGCATGGGGGCAGGGGCCTGGGAGTGCCCTCTCTTGCTAACATATG
  GCTAACAAAAATTCCTGTTTGAAGAAGGAGGCCCCGTCATCTAACCAGTTTTCTTTTTAC
  TTAAAAACAGAAAAAGTGAAAATTGTGTTACTCTGATCCTTGACCTCCGGGACTCTTCTG
  AGTTTTTTGGCCTGTTCCGGTGTTCCTTTCCCCTGCCTTACTTCCCCTGGGCGGCCTTGT
  TTTTTGGGGCCTTCTTTGAAGCTCGCTGTCTTTTTGGCCGGGCCTGAAGAATTTTAAACC
  CTTTCTGCCCTAGCTTGCAAGGCTCTTTAAAAAGGCCTTCGGAATTTCTCTTGGCTTAGC
  CCTCTGTCTTGGGTTCCTCCCAAAAGCTTTTGGACCCTTCCCCCCCTCGGTGTCTCTTTN
  TCCCCGGGCCCGCCCCTTTCTCTCTGCCCGTTTCGCGTGGTCTCTCCGCCCGGTCATATC
  TCGTTTTCTTTTTTCCCACCCCCGGTTTCCTTTGACTAAGCGCTTCTCCTATCCTCCACT
  TTATGAGACTACTCTTTTGGCGGTCCACTCCTCTCTTCTTCCGCCCGTCCTCCTCGGTCC
  CTGCCCGTCTCTCCTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021076
• Insert Size: 3740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021076.2, NP_066554.2
• RefSeq Size: 3695 bp
• RefSeq ORF: 3063 bp
• Locus ID: 4744
• UniProt ID: P12036
• Cytogenetics: 22q12.2
• Domains: filament
• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• Gene Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]