NARS1 (NM_004539) Human Untagged Clone

CAT#: SC110958

NARS (untagged)-Human asparaginyl-tRNA synthetase (NARS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NARS1
• Synonyms: ASNRS; NARS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110958 sequence for NM_004539 edited (data generated by NextGen Sequencing)
  ATGGTGCTAGCAGAGCTGTACGTCTCTGACCGAGAGGGAAGCGATGCCACGGGAGATGGA
  ACCAAGGAGAAACCATTTAAAACAGGTCTAAAGGCTTTGATGACAGTAGGGAAAGAACCA
  TTTCCTACCATTTACGTAGATTCACAAAAAGAAAATGAGAGGTGGAATGTTATTTCTAAA
  TCACAGTTGAAGAACATTAAAAAGATGTGGCATAGGGAACAAATGAAGAGTGAATCCCGG
  GAAAAGAAAGAGGCAGAAGATAGTTTACGAAGAGAAAAGAACCTGGAAGAAGCAAAGAAG
  ATTACCATTAAAAATGATCCAAGTCTCCCAGAGCCAAAATGTGTGAAGATTGGTGCGTTA
  GAAGGATATAGAGGCCAAAGAGTAAAGGTGTTTGGCTGGGTCCACAGGCTGCGCAGGCAA
  GGAAAGAATTTAATGTTTCTGGTGTTGCGAGATGGTACAGGTTATCTTCAGTGTGTCTTG
  GCGGATGAGTTGTGTCAGTGCTACAATGGAGTTCTCTTGTCCACGGAGAGCAGTGTTGCA
  GTGTATGGAATGCTAAATCTTACCCCAAAGGGCAAGCAGGCTCCAGGTGGCCATGAGCTG
  AGTTGTGACTTCTGGGAACTAATTGGGTTGGCCCCTGCTGGAGGAGCTGACAACCTGATC
  AATGAGGAGTCTGACGTTGATGTCCAGCTCAACAACAGACACATGATGATCCGAGGAGAA
  AACATGTCCAAAATCCTAAAAGCACGATCCATGGTCACCAGGTGCTTTAGAGATCACTTC
  TTTGATAGGGGGTACTATGAAGTTACTCCTCCAACATTAGTGCAAACACAAGTAGAAGGT
  GGTGCCACACTCTTCAAGCTTGACTATTTTGGGGAAGAGGCATTTTTGACTCAATCCTCT
  CAGTTGTACTTGGAGACCTGCCTCCCAGCCCTGGGAGATGTTTTTTGTATTGCTCAGTCA
  TACCGGGCAGAGCAGTCCAGAACACGAAGGCACCTGGCTGAGTACACTCACGTGGAAGCT
  GAGTGTCCTTTCCTGACTTTTGACGACCTCCTGAACCGGTTGGAGGACTTGGTTTGTGAT
  GTGGTAGATCGAATATTGAAGTCACCTGCAGGGAGCATAGTGCATGAGCTCAACCCGAAC
  TTTCAGCCCCCCAAACGGCCTTTCAAACGGATGAACTATTCAGATGCTATCGTTTGGCTA
  AAAGAACATGATGTAAAGAAAGAAGATGGAACTTTCTATGAATTTGGAGAAGATATCCCA
  GAAGCTCCTGAGAGACTGATGACAGACACCATTAATGAACCAATCTTGCTGTGTCGATTT
  CCTGTGGAGATCAAGTCCTTCTACATGCAGCGATGTCCTGAGGATTCCCGTCTTACTGAA
  TCTGTCGACGTGTTGATGCCCAATGTTGGTGAGATTGTGGGAGGCTCAATGCGTATCTTT
  GATAGTGAAGAAATACTGGCAGGTTATAAAAGGGAAGGGATTGACCCCACTCCCTATTAC
  TGGTATACGGATCAGAGAAAATACGGTACATGTCCCCATGGAGGATATGGCTTGGGCTTG
  GAACGATTCTTAACGTGGATTCTGAATAGGTATCACATCCGAGACGTGTGCTTATACCCT
  CGATTTGTCCAGCGTTGCACGCCATAA
  
  Clone variation with respect to NM_004539.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004539 unedited
  CGTGNCGCNCATTTTGTCAATACCGACNCACTTATAGGGCGGCCGCGATTCGGCACGAGG
  TGGAGGTGACCAGGGCCACTGCAGGCATGGTGCTAGCAGAGCTGTACGTCTCTGACCGAG
  AGGGAAGCGATGCCACGGGAGATGGAACCAAGGAGAAACCATTTAAAACAGGTCTAAAGG
  CTTTGATGACAGTAGGGAAAGAACCATTTCCTACCATTTACGTAGATTCACAAAAAGAAA
  ATGAGAGGTGGAATGTTATTTCTAAATCACAGTTGAAGAACATTAAAAAGATGTGGCATA
  GGGAACAAATGAAGAGTGAATCCCGGGAAAAGAAAGAGGCAGAAGATAGTTTACGAAGAG
  AAAAGAACCTGGAAGAAGCAAAGAAGATTACCATTAAAAATGATCCAAGTCTCCCAGAGC
  CAAAATGTGTGAAGATTGGTGCGTTAGAAGGATATAGAGGCCAAAGAGTAAAGGTGTTTG
  GCTGGGTCCACAGGCTGCGCAGGCAAGGAAAGAATTTAATGTTTCTGGTGTTGCGAGATG
  GTACAGGTTATCTTCAGTGTGTCTTGGCGGATGAGTTGTGTCAGTGCTACAATGGAGTTC
  TCTTGTCCACGGAGAGCAGTGTTGCAGTGTATGGAATGCTAAATCCTTACCCCAAAGGGC
  AAGCAGGCTCCAGGTGGCCATGAGCTGAGTTGTGACTTCTGGGAACTAATTGGGTTGGCC
  CCTGCTGGAGGAGCTGACCACCTGATCAATGANGAGTCTGACGTTGATGTCCAGCTCCAC
  AACAGACACATGATGATCCGAGGAGAAAACATGTCCAAATCCTAAAAGCACGATCCATGG
  TCACNCAGTGCTTTAGAGACACTTCTTTGATAGGGGTACTATGAAGNTACTCCTCCCACA
  TTAGTGCAAACCCAGGTANAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004539 unedited
  TCGGCGATGGGCACTTNCCAGGGCCGGNANAGGCACTGGGGNGGGGTCACAGGGATGCCA
  CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTT
  TTTTTTTTTTTAACGTTAGCAATATCTACTAAATTTAGTAGTACTTAATTTCACATATAT
  ACATCATAAAAAAGATAAAATAATTGCAAAAGCATGAACAAATGTGACAAAGAGATGTTT
  AGCTTTAAGAAAATTAAGCCAGCAAAGAGTTTTTACGGGGTGTTCATTTATTGACTGCTG
  GGAATACAGCCTATTGAGAATACATGACATGCATTTGGGGGAGAAATCAACTAAATCTGA
  AATGAAGCCATTCAGTAAGCCTGCTGTGAGTTAATAAGGTAGCACAGATTAGATCTAAGG
  ACATGGGTGGGGGCAACTCAAACAGCCACTCACAATTGTTATTAAGATTCCAGCTACAAT
  TTCTTTTGGAACTTATTTATTCTTAATTACTTAGGAATTACCAGGAAAGAGGGAAAATCA
  ATCTGCCTCTTTACCTTAAATTAATGCTAAGAGGGATACTGTGAATCACTAANAAACTCT
  CCCCAAAATTATGTTCTTACTTCAAATCCTTTAGGGCATTCTATTTTTACAAATTCTTCA
  ATTTCTATGCCCTCAGATTATTANCAGCTAAGCCGAGGTAGAAAAGATTAATAACTGATA
  CAAAGGCTATTCATTTGATTCTAGGGGTTACTGCTGATTAACCCGGGGTCCTAAAGGAGC
  AGATTTTATACGAATTGGCCATTTCTCCCCCAGAAATTTTGGTTTAATTCCATGAGCGNA
  AAATAATCAAGAATTCAGGTACCTTAAAGGAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004539
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004539.2, NP_004530.1
• RefSeq Size: 2714 bp
• RefSeq ORF: 1647 bp
• Locus ID: 4677
• UniProt ID: O43776
• Cytogenetics: 18q21.31
• Domains: tRNA-synt_2, tRNA_anti
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. [provided by RefSeq, Jul 2008]