KGF (FGF7) (NM_002009) Human Untagged Clone

CAT#: SC110894

FGF7 (untagged)-Human fibroblast growth factor 7 (FGF7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KGF
• Synonyms: HBGF-7; KGF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110894 sequence for NM_002009 edited (data generated by NextGen Sequencing)
  ATGCACAAATGGATACTGACATGGATCCTGCCAACTTTGCTCTACAGATCATGCTTTCAC
  ATTATCTGTCTAGTGGGTACTATATCTTTAGCTTGCAATGACATGACTCCAGAGCAAATG
  GCTACAAATGTGAACTGTTCCAGCCCTGAGCGACACACAAGAAGTTATGATTACATGGAA
  GGAGGGGATATAAGAGTGAGAAGACTCTTCTGTCGAACACAGTGGTACCTGAGGATCGAT
  AAAAGAGGCAAAGTAAAAGGGACCCAAGAGATGAAGAATAATTACAATATCATGGAAATC
  AGGACAGTGGCAGTTGGAATTGTGGCAATCAAAGGGGTGGAAAGTGAATTCTATCTTGCA
  ATGAACAAGGAAGGAAAACTCTATGCAAAGAAAGAATGCAATGAAGATTGTAACTTCAAA
  GAACTAATTCTGGAAAACCATTACAACACATATGCAKCAGCTAAATGGACACACAACGGA
  GGGGAAATGTTTGTKGCCTTAAATCAAAAGGGGATTCCTGTAAGAGGAAAAAAAACGAAG
  AAAGAACAAAAAACAGCCCACTTTCTTCCTATGGCAATAACTTAA
  
  Clone variation with respect to NM_002009.3
  457 t=>k;495 t=>k
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002009 unedited
  GTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCACGCGCTCAC
  ACACAGAGAGAAAATCCTTCTGCCTGTTNGATTTATGGAAACAATTATGATTCTGCTGGA
  GAACTTTTCAGCTGAGAAATAGTTTGTAGCTACAGTAGAAAGGCTCAAGTTGCACCAGGC
  AGACAACAGACATGGAATTCTTATATATCCAGCTGTTAGCAACAAAACAAAAGTCAAATA
  GCAAACAGCGTCACAGCAACTGAACTTACTACGAACTGTTTTTATGAGGATTTATCAACA
  GAGTTATTTAAGGAGGAATCCTGTGTTGTTATCAGGAACTAAAAGGATAAGGCTAACAAT
  TTGGAAAGAGCAACTACTCTTTCTTAAATCAATCTACAATTCACAGATAGGAAGAGGTCA
  ATGACCTAGGAGTAACAATCAACTCAAGATTCATTTTCATTATGTTATTCATGAACACCC
  GGAGCACTACACTATAATGCACAAATGGATACTGACATGGATCCTGCCAACTTTGCTCTA
  CAGATCATGCTTTCACATTATCTGTCTAGTGGGTACTATATCTTTAGCTTGCAATGACAT
  GACTCCAGAGCAAATGGCTACAAATGTGAACTGTTCCAGCCCTGAGCGACACACAAGAAG
  TTATGATTACATGGAAGGAGGGGATATAAGAGTGAGAAGACTCTTCTGTCGAACACAGTG
  GTACCTGAGGATCGATAAAAGAGGCAAAGTAAAAGGGACCCAAGAGATGAAGAATAATTA
  CAATATCATGGAAATCAGGACAGTGGCAGTTGGAATTGTGGCATCAAAGGGTGGAAGTGA
  ATTCTATCTGCATGAACAGGNANGAAAACTCTATGCAAGAAGATGCAATGAGATTGTACT
  TCAAGACTATTCTGGAAACCATTCACACTATGCTCAGCTAATGGACACACACGAGGGGAA
  ATGTTGTGCCTAATAAAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002009 unedited
  ATGGAACGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTCTAATTGTTAGAA
  ACTCATGTATTTAATTTTATCTTTCAAAGAACATTTTTAAAAGGCTTACTCTGCTTATAT
  GTATATTTTGCACAAAGCTTTCCATTCATAAACAAAAGGTGAATAGGAATCAGAATTGAA
  AACTACATAATTTCTTTTCTCCTTTTGAGTCAAATCATCACAGTATAGATTAGTCATTGT
  GTATGAAGTGCATTGCCAAGACATAGTCACATTTGTGTACAAATGTAAAGGCTATATCTC
  AACAGAAATAAAAAACAAAAACATGCCAAAGACCATTAGCTAATAATACTAATAATCGAA
  GAGTAGAAAGCATGTGCTAACTGATCTAGACTCTTGTGACAGCTGTATATCTACCCAGCT
  GCGTGACCTTAGGTGTATCAAAATCTCAATTTCCTATACTACAAATGAGTAAGAGGGATG
  GAGGCCCCTCACAGTTTTAATATTCTAGAATTCTAAAGGTGCGTAACTAGAGCAAATTTT
  ATTTTCTAAATTTGCATACTAAATTTAAAATAGGAGAAAAACAAATAATTAAAGTACATA
  TTTCTAGAAGTCTTTTTAGGCGACTTATACTACCAAGAATGGATTTACCACTAAATGTAG
  GTTTTGCCAATTCCAACTTTGCTATGTACTCTGATGGAAGATAACTCCATAGACAAAGAC
  ATTTGGCCCAAACTAACTTGAATTTATATGCATATACACTATTCCTATTAAATTCCCGGA
  TAGACAAGTCTAACCCTTTAAAAGAAAAATAACCTAAAGGGAATTTCCTTTGGCTATAAA
  CTTCAGGGAATCCCCTGGAAACCGTTTATAAACAAACGCCGCTAGGCCTAAGGAACCCAC
  TGAAAACTATTCCCCCCCCGCCCCATATCCACAAAATAACTTCTTAACCCGACCTAAACA
  TATAGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002009
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002009.2, NP_002000.1
• RefSeq Size: 3853 bp
• RefSeq ORF: 585 bp
• Locus ID: 2252
• UniProt ID: P21781
• Cytogenetics: 15q21.2
• Domains: FGF
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]