Tissue Factor (F3) (NM_001993) Human Untagged Clone

CAT#: SC110888

F3 (untagged)-Human coagulation factor III (thromboplastin, tissue factor) (F3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Tissue Factor
• Synonyms: CD142; TF; TFA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110888 sequence for NM_001993 edited (data generated by NextGen Sequencing)
  ATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCCGAGACCGCCGTCGCTCGGACGCTC
  CTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCTTCAGGCACTACAAATACTGTGGCA
  GCATATAATTTAACTTGGAAATCAACTAATTTCAAGACAATTTTGGAGTGGGAACCCAAA
  CCCGTCAATCAAGTCTACACTGTTCAAATAAGCACTAAGTCAGGAGATTGGAAAAGCAAA
  TGCTTTTACACAACAGACACAGAGTGTGACCTCACCGACGAGATTGTGAAGGATGTGAAG
  CAGACGTACTTGGCACGGGTCTTCTCCTACCCGGCAGGGAATGTGGAGAGCACCGGTTCT
  GCTGGGGAGCCTCTGTATGAGAACTCCCCAGAGTTCACACCTTACCTGGAGACAAACCTC
  GGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGAACAAAAGTGAATGTGACCGTAGAA
  GATGAACGGACTTTAGTCAGAAGGAACAACACTTTCCTAAGCCTCCGGGATGTTTTTGGC
  AAGGACTTAATTTATACACTTTATTATTGGAAATCTTCAAGTTCAGGAAAGAAAACAGCC
  AAAACAAACACTAATGAGTTTTTGATTGATGTGGATAAAGGAGAAAACTACTGTTTCAGT
  GTTCAAGCAGTGATTCCCTCCCGAACAGTTAACCGGAAGAGTACAGACAGCCCGGTAGAG
  TGTATGGGCCAGGAGAAAGGGGAATTCAGAGAAATATTCTACATCATTGGAGCTGTGGTA
  TTTGTGGTCATCATCCTTGTCATCATCCTGGCTATATCTCTACACAAGTGTAGAAAGGCA
  GGAGTGGGGCAGAGCTGGAAGGAGAACTCCCCACTGAATGTTTCATAA
  
  Clone variation with respect to NM_001993.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001993 unedited
  TACTATAGGGNCGGCACGCGCAATTCGGCACGAGGCCCGCACCCCCTCGCACTCCCTCTG
  GCCGGCCCAGGGCGCCTTCAGCCCAACCTCCCCAGCCCCACGGGCGCCACGGAACCCGCT
  CGATCTCGCCGCCAACTGGTAGACATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCC
  GAGACCGCCGTCGCTCGGACGCTCCTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCT
  TCAGGCACTACAAATACTGTGGCAGCATATAATTTAACTTGGAAATCAACTAATTTCAAG
  ACAATTTTGGAGTGGGAACCCAAACCCGTCAATCAAGTCTACACTGTTCAAATAAGCACT
  AAGTCAGGAGATTGGAAAAGCAAATGCTTTTACACAACAGACACAGAGTGTGACCTCACC
  GACGAGATTGTGAAGGATGTGAAGCAGACGTACTTGGCACGGGTCTTCTCCTACCCGGCA
  GGGAATGTGGAGAGCACCGGTTCTGCTGGGGAGCCTCTGTATGAGAACTCCCCAGAGTTC
  ACACCTTACCTGGAGACAAACCTCGGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGA
  ACAAAAGTGAATGTGACCGTAGAAGATGAACGGACTTTAGTCAGAAGGAACAACACTTTC
  CTAAGCCTCCGGGATGTTTTTGGGCAGGACTTAATTTATACACTTTATTATTGGAAAATC
  TCAAGTTCAGGAAAGAAAACAGCCAANACAAACACTAATGAGTTTTTGATTGATGTGGAT
  AAAGGAGAAACTACTGNNTTCAGTGTCAAGCAGTGATTCCCTCCCGACAGTTAACCGNAG
  ATACAGACGCCCGGTAGATGTATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001993 unedited
  AGCTATGGACCGCGGCCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTACAATTCCCAGTCACCTTTATTTAAAGTATATTAAATAAATTATCTCAATATATA
  CAAATAGAACAATATTACCTACATAAATAGAAAATCCCCATATAGAAAACCTTTTTAAAA
  AATTATATATACAATGTCAACCATAGAAGCTTTAAGTACCTTAAATCATAAACTCTGTAG
  TTTGTATAGTAGTCTTATAAAAATATAGTTAGCTCTCAAATGTTTAAGGCCACTTAAGTC
  AGTTAAAGGGCAGATTGTAAAGCATATTAGGAAGGTGCCCAGAATACCAATGTCTCCTGC
  ACTTAACACATTAATACAAAGTTTGCCAATTGTTTTGAATTTCCAAATGTATCCCTGAAA
  AAAAAAAGAACCTAAACACTATATTATAGACATACGTTAGAAAAGTCCTAGAAATGCACC
  CAATTTCCTTCCATTTTACTTTCCTACACGGATTGAAGCCAGCCCCTCAAAAGCTTTTCG
  GCTGGGCATGGTGGTTCACGCCCATAATACTAGCACTTTGGGAGGCCAAGGTGGGTGGAT
  CAACTGAGGTCAGGAATTCAAGACCAGCCTGGCCAAGATGGCGAAACCCCCATCTCTACC
  TAAAAAATACAAAAATTAGCCTGGCGTGGTAGTGCGCACCTGGAAATCCCAGCTACTCGG
  GGAGGCTGAGGCAGACAATTGCTTTGACCCCCAGAGACGGAGGGTGCAGTGGAGCCAGAG
  ATCGGGCTACTGCACTTCAACCGGGGGCAAAAGAGCAAGAATCCGTCTAAAAAAAAAAAA
  AAAAAAGGCTTTTAAAGGCCCACCAGAATTTTAAGAAATTTCCCTTTGTTTTGCTGGAAG
  ACCGCTACCTCTGGGGCGGAAAAAATTTAAGCCGTTATGGCAAAGAGCCCAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001993
• Insert Size: 2240 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001993.2, NP_001984.1
• RefSeq Size: 2153 bp
• RefSeq ORF: 888 bp
• Locus ID: 2152
• UniProt ID: P13726
• Cytogenetics: 1p21.3
• Domains: Tissue_fac
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.