E2F3 (NM_001949) Human Untagged Clone

CAT#: SC110876

E2F3 (untagged)-Human E2F transcription factor 3 (E2F3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: E2F3
• Synonyms: E2F-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110876 sequence for NM_001949 edited (data generated by NextGen Sequencing)
  ATGAGAAAGGGAATCCAGCCCGCTCTGGAGCAGTACCTGGTGACCGCCGGGGGTGGGGAG
  GGGGCGGCTGTCGTCGCCGCCGCCGCTGCAGCCTCCATGGACAAAAGGGCACTGCTAGCC
  AGCCCCGGCTTCGCCGCCGCCGCCGCCGCTGCCGCCGCCCCGGGCGCGTACATCCAGATC
  CTCACCACGAACACTTCCACCACCTCCTGTTCCTCCTCCCTCCAAAGCGGCGCCGTAGCC
  GCCGGCCCCCTCCTCCCCAGTGCCCCCGGCGCGGAGCAGACCGCCGGCAGCCTCCTCTAC
  ACCACGCCGCACGGACCCTCCAGCAGAGCCGGGCTGCTGCAGCAGCCACCAGCGCTGGGA
  CGCGGCGGCAGCGGCGGCGGCGGCGGCCCTCCGGCAAAGCGAAGGCTGGAGCTAGGAGAA
  AGCGGTCATCAGTACCTCTCAGATGGTTTAAAAACCCCCAAGGGCAAAGGAAGAGCTGCA
  CTACGAAGTCCAGATAGTCCAAAAACTCCAAAATCTCCCTCAGAAAAAACGCGGTATGAT
  ACGTCTCTTGGTCTGCTCACCAAGAAGTTCATTCAGCTCCTGAGCCAGTCACCCGATGGG
  GTATTGGATTTGAACAAGGCAGCAGAAGTGCTAAAAGTGCAAAAGAGAAGGATTTATGAT
  ATCACCAACGTTCTGGAAGGCATCCACCTCATTAAGAAGAAGTCTAAAAACAACGTCCAA
  TGGATGGGCTGCAGTCTGTCTGAGGATGGGGGCATGCTGGCCCAGTGTCAAGGCCTGTCA
  AAAGAAGTGACCGAGCTCAGTCAGGAAGAGAAGAAATTAGATGAACTGATCCAAAGCTGC
  ACCCTGGACCTCAAACTGTTAACCGAGGATTCAGAGAATCAAAGGTTAGCTTATGTTACA
  TATCAAGATATTCGAAAAATTAGTGGCCTTAAAGACCAAACTGTTATAGTTGTGAAAGCC
  CCTCCAGAAACAAGACTTGAAGTGCCTGACTCAATAGAGAGCCTACAAATACATTTGGCA
  AGTACCCAAGGGCCCATTGAGGTTTACTTATGTCCAGAAGAGACTGAAACACACAGTCCA
  ATGAAAACAAACAACCAAGACCACAATGGGAATATCCCTAAACCCGCTTCCAAAGACTTG
  GCTTCAACCAACTCAGGACATAGCGATTGCTCAGTTTCTATGGGAAACCTTTCTCCTCTG
  GCCTCCCCAGCCAACCTCTTACAGCAGACTGAGGACCAAATTCCTTCCAACCTAGAAGGA
  CCGTTTGTGAACTTACTGCCTCCCCTGCTGCAAGAGGACTATCTCCTGAGCCTCGGGGAG
  GAGGAAGGCATCAGCGATCTCTTCGATGCTTACGATTTGGAAAAGCTCCCACTGGTGGAA
  GACTTCATGTGTAGTTGA
  
  Clone variation with respect to NM_001949.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001949 unedited
  GGGTACTATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCGAGGGAAAAC
  AATACATTAATATACCATAACACTAAAAAGAGCAGGAGCGAGAGATGAGAAAGGGAATCC
  AGCCCGCTCTGGAGCAGTACCTGGTGACCGCCGGGGGTGGGGAGGGGGCGGCTGTCGTCG
  CCGCCGCCGCTGCATCCTCCATGGACAAAAGGGCACTGCTAGCCAGCCCCGGCTTCGCCG
  CCGCAGACCCTCATGACTCAACTTCTCCTCCTGCTACAGGCACCATATCACATCAAGCCC
  CATCCCTCTCTGCCTAGATCATACTTTCTCTCTTCCTCTCCCAACTACCTATCACTACGT
  TCCATCTCTAATTCAATTTGCGCACTCCACCAGATACCAACCCCTCCCACCGCTTCCCTA
  CCTCTCACCTCCTCAACCTTATCATTTTCTTTTACACAATCATCATCTTCTAACCCGCAC
  GATCCCCGTTCCCATACACTTTCACAACCCCTCTGACCGCCTCACCACTCACTCCTCGCT
  ACCATTGCTTCCCTTCTCCCCTTTTTACTACACTTCACTCCTCTTCTCTCTATCCATCTT
  AGCTCCCCTCTTTTTTACCTTATCCATACCTCTATTTCACTCCTCTCTTATGATTTTTTT
  CTTTTTTCTCCCATAACTATTTTCCTCTCCCCTTCCCTCACTCCCCTTCCTTTTACCTAC
  TCCGCACCCACTTTACTACTCCCCTCCTCAACACGCTCATTACTTCTCCTTCATTACGTG
  GTTTTCGTCCTCTTNTCCATCCTTTCCCTCTGTATTCCCAGTAGTTACTCCTCCTCTCTC
  CCCTACACAACTTTCCATCACCCANAAACACACACCCTCAGGCTCTTACTAGGCTCACCT
  CCTCAACTCTTATACCCTCACATTTTCATCCCCTTACACCTCTCTCTCCTCCTTCTGTTC
  TCATCCTCCCTCTCTTCTCCCCNTCTTGTCTATCCCACCTCCCTACGTGCTCCTTTCCGT
  ATCCTTTCCTCTCCTATATCTCCCACCGATCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001949 unedited
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTAAGGATATTTTCAATTCAATTAAA
  CATATGAGTTGAGTGCCTACTATGTGCCTACCATTGGGTCAGGCACATAGCATTTCAATT
  TAACTGTTCGTTTCGAGGTTTGTTCTTTATCTGACTCCCTCATTCCCGCAGTCCTTATCA
  TCCAAGGAATGGACCGGAGATACTACGCCATATTCCCAGTGGGAGATGGCCGCTGCCACC
  TGCCCGAGGGAAAGCCATTCTTGGACATAACTGGTAGGATTTGCAGCTGACACAAAGCCT
  CCAACTTGGGACACCATTCCTGAAGAGTAAAGCGGGAGACACCCTGGCATTGTTTTGCTT
  CATTTTTTACGTCCCCGACTTCCCTTGGTCCAACTTATTCCTTCATAGAGGAAGGGGAAC
  GGGGTCTGGCCAGAACTATTCCAGCCCCCGACACAGGGCTGCTCCCTGTTCCTGCCAGCT
  TAAGGAAATGCCACTCACACAATGGGGATGTCTTTCTCCCTAGAGGTCTCTCCATCTGAC
  CCCATCCTGAGAAGTGGTCTCCCTGAGTGCGGTGTAAGAGAGCCCTTTCCCCTGTCACAG
  GGGCCCCGGGAAGCCCAAGGATGGAGGGACCCATGCCTGCTGCTCCTTGAGGCTGTTACA
  TCATCTGACCTGGTTCTCCTTTCCATGGGCCACCCATGTCAGCAAGCCCCATGACTGACT
  CGCTCCAAGCTCCCCCTNCTGGGAATGGNAGCACGATGGGACAAAGCTCCTTGCAGGAGC
  TGANTAACCCTANATCACACAGGTCTTAGATGCTCTGATCAGTAAAGGNTCTCTCCCTCT
  GCCTAATAATATGAATTNGCAAAACCCGCCTCAAAGTAAACATTTAAATCGGAATGGGGG
  ATGGCTGCCCCAGGAAGATGCTAATGGCACGCTAAAAAATTTAAAGCCTAGAACATCCCA
  AAAGAACTTAGTAGGCGTTTGCTACAATCANCTGGACTNCCAAACCATGGGGACGGGGAA
  TGGNATGGTCATGAAACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001949
• Insert Size: 3150 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001949.2, NP_001940.1
• RefSeq Size: 4744 bp
• RefSeq ORF: 1398 bp
• Locus ID: 1871
• UniProt ID: O00716
• Cytogenetics: 6p22.3
• Domains: E2F_TDP
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer
• Gene Summary: This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
  Transcript Variant: This variant (1, also known as a) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.