Antithrombin III (SERPINC1) (NM_000488) Human Untagged Clone

CAT#: SC110831

SERPINC1 (untagged)-Human serpin peptidase inhibitor, clade C (antithrombin), member 1 (SERPINC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Antithrombin III
• Synonyms: AT3; AT3D; ATIII; ATIII-R2; ATIII-T1; ATIII-T2; THPH7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110831 sequence for NM_000488 edited (data generated by NextGen Sequencing)
  ATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGAAGGTTTATCTTTTGTCC
  TTGCTGCTCATTGGCTTCTGGGACTGCGTGACCTGTCACGGGAGCCCTGTGGACATCTGC
  ACAGCCAAGCCGCGGGACATTCCCATGAATCCCATGTGCATTTACCGCTCCCCGGAGAAG
  AAGGCAACTGAGGATGAGGGCTCAGAACAGAAGATCCCGGAGGCCACCAACCGGCGTGTC
  TGGGAACTGTCCAAGGCCAATTCCCGCTTTGCTACCACTTTCTATCAGCACCTGGCAGAT
  TCCAAGAATGACAATGATAACATTTTCCTGTCACCCCTGAGTATCTCCACGGCTTTTGCT
  ATGACCAAGCTGGGTGCCTGTAATGACACCCTCCAGCAACTGATGGAGGTATTTAAGTTT
  GACACCATATCTGAGAAAACATCTGATCAGATCCACTTCTTCTTTGCCAAACTGAACTGC
  CGACTCTATCGAAAAGCCAACAAATCCTCCAAGTTAGTATCAGCCAATCGCCTTTTTGGA
  GACAAATCCCTTACCTTCAATGAGACCTACCAGGACATCAGTGAGTTGGTATATGGAGCC
  AAGCTCCAGCCCCTGGACTTCAAGGAAAATGCAGAGCAATCCAGAGCGGCCATCAACAAA
  TGGGTGTCCAATAAGACCGAAGGCCGAATCACCGATGTCATTCCCTCGGAAGCCATCAAT
  GAGCTCACTGTTCTGGTGCTGGTTAACACCATTTACTTCAAGGGCCTGTGGAAGTCAAAG
  TTCAGCCCTGAGAACACAAGGAAGGAACTGTTCTACAAGGCTGATGGAGAGTCGTGTTCA
  GCATCTATGATGTACCAGGAAGGCAAGTTCCGTTATCGGCGCGTGGCTGAAGGCACCCAG
  GTGCTTGAGTTGCCCTTCAAAGGTGATGACATCACCATGGTCCTCATCTTGCCCAAGCCT
  GAGAAGAGCCTGGCCAAGGTGGAGAAGGAACTCACCCCAGAGGTGCTGCAGGAGTGGCTG
  GATGAATTGGAGGAGATGATGCTGGTGGTCCACATGCCCCGCTTCCGCATTGAGGACGGC
  TTCAGTTTGAAGGAGCAGCTGCAAGACATGGGCCTTGTCGATCTGTTCAGCCCTGAAAAG
  TCCAAACTCCCAGGTATTGTTGCAGAAGGCCGAGATGACCTCTATGTCTCAGATGCATTC
  CATAAGGCATTTCTTGAGGTAAATGAAGAAGGCAGTGAAGCAGCTGCAAGTACCGCTGTT
  GTGATTGCTGGCCGTTCGCTAAACCCCAACAGGGTGACTTTCAAGGCCAACAGGCCTTTC
  CTGGTTTTTATAAGAGAAGTTCCTCTGAACACTATTATCTTCATGGGCAGAGTAGCCAAC
  CCTTGTGTTAAGTAA
  
  Clone variation with respect to NM_000488.3
  981 a=>g;1011 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000488 unedited
  TCAGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGCTCCACTT
  GCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCT
  GGAAAAAGGAAGGTTTATCTTTTGTCCTTGCTGCTCATTGGCTTCTGGGACTGCGTGACC
  TGTCACGGGAGCCCTGTGGACATCTGCACAGCCAAGCCGCGGGACATTCCCATGAATCCC
  ATGTGCATTTACCGCTCCCCGGAGAAGAAGGCAACTGAGGATGAGGGCTCAGAACAGAAG
  ATCCCGGAGGCCACCAACCGGCGTGTCTGGGAACTGTCCAAGGCCAATTCCCGCTTTGCT
  ACCACTTTCTATCAGCACCTGGCAGATTCCAAGAATGACAATGATAACATTTTCCTGTCA
  CCCCTGAGTATCTCCACGGCTTTTGCTATGACCAAGCTGGGTGCCTGTAATGACACCCTC
  CAGCAACTGATGGAGGTATTTAAGTTTGACACCATATCTGAGAAAACATCTGATCAGATC
  CACTTCTTCTTTGCCAAACTGAACTGCCGACTCTATCGAAAAGCCAACAAATCCTCCAAG
  TTAGTATCAGCCAATCGCCTTTTTGGAGACAAATCCCTTACCTTCAATGAGACCTACCAG
  GACATCAGTGAGTTGGTATATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGAAAATGCA
  GAGCAATCCAGAGCGGCCATCAACAAATGGGGTGTCCAATAAGACCGAAGGCCGAATCAC
  CGATGTCATTCCCTTCGAAGCCATCAATGAGCTCACTGGNTCTGGGTGCTGGNTAACACC
  ATTTACTTCAGGGGCCTGTGAAGTCAAAGTTCAGCCCTGAGACACANGNAGGAACTGTTC
  TACAGGCTGATGGANAGTCGTGTCACATCTTGATGTACAGNAAGGCAAGTCCGTTATGGC
  GCGTGCTGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000488 unedited
  NNTTTTTACTCTGNACCGCGGNCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTAATGGGAGAAGGAAGTAGTTTGTATTTATTTTTACTTCTG
  TTCACAAACCAAAAATAGGAAGAGGGGCAAAAAATAAAAACATTTTACTTAACACAAGGG
  TTGGCTACTCTGCCCATGAAAAAATAGTGTTCAAAGGAACTTCTTTTATAAAAACCAAAA
  AAGGCCTGTTGGCCTTGAAAGTCACCCTGTTGGGGTTTAGCGAACGGCCAGCAATCACAA
  CAGCGGTACTTGCAGCTGCTTCACTGCCTTCTTCATTTACCTCAAAAAATGCCTTATGGA
  ATGCATCTGAAACATAAAGGTCATTTCGGCCTTTTGCAACAATACCTGGGAGTTTGGACT
  TTTCAGGGCTGAACAAATCGACAAGGCCCATGTTTTGCAACTGCTCCTTCAAACTGAAGC
  CGTCCTCAATGCGGAAGCGGGGCATGTGGACCACCAGCATCATTTCCTCCAATTCATCCA
  GCCACTCCTGCAGCACCTTTGGGGTGAGTTCCTTCTCCACCTTGGCCAGGCTCTTCTCAG
  GCTTGGGCAAAATGAGGACCATGGTGATGTCATCACCTTTGAAGGGCAACTCAAGCACCT
  GGGTGCCTTCAGCCACGCGCCGATAACGGAACTTGCCTTCCTGGTACATCATAGATGCTG
  AACACGACTCTCCATCAGCCTTGTAGACAGTTCCTTCCTTGTGTTCTCAGGGCTGAACTT
  TGACTTCCACAGCCCTTGAGTAAATGGGTGTTAACCAGCACAGAACAGTGAGCTCATTGA
  TGGCTTCCGAGGGAATGACATCGGNGATTCGGCCTTTCGTCTTATTGAAACCCATTTGTT
  GATGGCCCGTCTGAATGCTCTGCATTTCCCTGAAGTCAGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000488
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000488.2, NP_000479.1
• RefSeq Size: 1559 bp
• RefSeq ORF: 1395 bp
• Locus ID: 462
• UniProt ID: P01008
• Cytogenetics: 1q25.1
• Domains: SERPIN
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: The protein encoded by this gene, antithrombin III, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. Numerous mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency which constitutes a strong risk factor for thrombosis. A reduction in the serum level of this protein is associated with severe cases of Coronavirus Disease 19 (COVID-19). [provided by RefSeq, Sep 2020]