TAP2 (NM_000544) Human Untagged Clone

CAT#: SC109816

TAP2 (untagged)-Human transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAP2
• Synonyms: ABC18; ABCB3; APT2; D6S217E; PSF-2; PSF2; RING11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000544, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGGCTCCCTGACCTGAGACCCTGGACCTCCCTGCTGCTGGTGGACGCGGCTTTACTGTGGCTGCTTC
  AGGGCCCTCTGGGGACTTTGCTTCCTCAAGGGCTGCCAGGACTATGGCTGGAGGGGACCCTGCGGCTGGG
  AGGGCTGTGGGGGCTGCTAAAGCTAAGAGGGCTGCTGGGATTTGTGGGGACACTGCTGCTCCCGCTCTGT
  CTGGCCACCCCCCTGACTGTCTCCCTGAGAGCCCTGGTCGCGGGGGCCTCACGTGCTCCCCCAGCCAGAG
  TCGCTTCAGCCCCTTGGAGCTGGCTGCTGGTGGGGTACGGGGCTGCGGGGCTCAGCTGGTCACTGTGGGC
  TGTTCTGAGCCCTCCTGGAGCCCAGGAGAAGGAGCAGGACCAGGTGAACAACAAAGTCTTGATGTGGAGG
  CTGCTGAAGCTCTCCAGGCCGGACCTGCCTCTCCTCGTTGCCGCCTTCTTCTTCCTTGTCCTTGCTGTTT
  TGGGTGAGACATTAATCCCTCACTATTCTGGTCGTGTGATTGACATCCTGGGAGGTGATTTTGACCCCCA
  TGCCTTTGCCAGTGCCATCTTCTTCATGTGCCTCTTCTCCTTTGGCAGCTCACTGTCTGCAGGCTGCCGA
  GGAGGCTGCTTCACCTACACCATGTCTCGAATCAACTTGCGGATCCGGGAGCAGCTTTTCTCCTCCCTGC
  TGCGCCAGGACCTCGGTTTCTTCCAGGAGACTAAGACAGGGGAGCTGAACTCACGGCTGAGCTCGGATAC
  CACCCTGATGAGTAACTGGCTTCCTTTAAATGCCAATGTGCTCTTGCGAAGCCTGGTGAAAGTGGTGGGG
  CTGTATGGCTTCATGCTCAGCATATCGCCTCGACTCACCCTCCTTTCTCTGCTGCACATGCCCTTCACAA
  TAGCAGCGGAGAAGGTGTACAACACCCGCCATCAGGAAGTGCTTCGGGAGATCCAGGATGCAGTGGCCAG
  GGCGGGGCAGGTGGTGCGGGAAGCCGTTGGAGGGCTGCAGACCGTTCGCAGTTTTGGGGCCGAGGAGCAT
  GAAGTCTGTCGCTATAAAGAGGCCCTTGAACAATGTCGGCAGCTGTATTGGCGGAGAGACCTGGAACGCG
  CCTTGTACCTGCTCGTAAGGAGGGTGCTGCACTTGGGGGTGCAGATGCTGATGCTGAGCTGTGGGCTGCA
  GCAGATGCAGGATGGGGAGCTCACCCAGGGCAGCCTGCTTTCCTTTATGATCTACCAGGAGAGCGTGGGG
  AGCTATGTGCAGACCCTGGTATACATATATGGGGATATGCTCAGCAACGTGGGAGCTGCAGAGAAGGTTT
  TCTCCTACATGGACCGACAGCCAAATCTGCCTTCACCTGGCACGCTTGCCCCCACCACTCTGCAGGGGGT
  TGTGAAATTCCAAGACGTCTCCTTTGCATATCCCAATCGCCCTGACAGGCCTGTGCTCAAGGGGCTGACG
  TTTACCCTACGTCCTGGTGAGGTGACGGCGCTGGTGGGACCCAATGGGTCTGGGAAGAGCACAGTGGCTG
  CCCTGCTGCAGAATCTGTACCAGCCCACAGGGGGACAGGTGCTGCTGGATGAAAAGCCCATCTCACAGTA
  TGAACACTGCTACCTGCACAGCCAGGTGGTTTCAGTTGGGCAGGAGCCTGTGCTGTTCTCCGGTTCTGTG
  AGGAACAACATTGCTTATGGGCTGCAGAGCTGCGAAGATGATAAGGTGATGGCGGCTGCCCAGGCTGCCC
  ACGCAGATGACTTCATCCAGGAAATGGAGCATGGAATATACACAGATGTAGGGGAGAAGGGAAGCCAGCT
  GGCTGCGGGACAGAAACAACGTCTGGCCATTGCCCGGGCCCTTGTACGAGACCCGCGGGTCCTCATCCTG
  GATGAGGCTACTAGTGCCCTAGATGTGCAGTGCGAGCAGGCCCTGCAGGACTGGAATTCCCGTGGGGATC
  GCACAGTGCTGGTGATTGCTCACAGGCTGCAGGCAGTTCAGCGCGCCCACCAGATCCTGGTGCTCCAGGA
  GGGCAAGCTGCAGAAGCTTGCCCAGCTCCAGGAGGGACAGGACCTCTATTCCCGCCTGGTTCAGCAGCGG
  CTGATGGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000544 unedited
  CACAATTTGTATACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGCGGTAGCGAGG
  TTGGGAGAGACGGAGCGGACCTCAGCGCTGAAGCAGAAGCCCCCGGAGCTGCGGTCTCCC
  CGCCGCGGCTGAGCCATGCGGCTCCCTGACCTGAGACCCTGGACCTCCCTGCTGCTGGTG
  GACGCGGCTTTACTGTGGCTGCTTCAGGGCCCTCTGGGGACTTTGCTTCCTCAAGGGCTG
  CCAGGACTATGGCTGGAGGGGACCCTGCGGCTGGGAGGGCTGTGGGGGCTGCTAAAGCTA
  AGAGGGCTGCTGGGATTTGTGGGGACACTGCTGCTCCCGCTCTGTCTGGCCACCCCCCTG
  ACTGTCTCCCTGAGAGCCCTGGTCGCGGGGGCCTCACGTGCTCCCCCAGCCAGAGTCGCT
  TCAGCCCCTTGGAGCTGGCTGCTGGTGGGGTACGGGGCTGCGGGGCTCAGCTGGTCACTG
  TGGGCTGTTCTGAGCCCTCCTGGAGCCCAGGAGAAGGAGCAGGACCAGGTGAACAACAAA
  GTCTTGATGTGGAGGCTGCTGAAGCTCTCCAGGCCGGACCTGCCTCTCCTCGTTGCCGCC
  TTCTTCTTCCTTGTCCTTGCTGTTTTGGGTGAGACATTAATCCCTCACTATTCTGGTCGT
  GTGATTGACATCCTGNGAGGTGATTTTGACCCCCATGCCTTTGCCAGTGCCATCTTCTTC
  ATGTGCCCTCTCTCCTTTGGCAGCTCACTGTCTGCANGCTGCCCGAGAGGCTGCTTCACC
  TACACCATGTCTCGAATCAACTTGCNGATCCGGGAGCAGCTTTTCTCCTCCCTGCTGCGC
  CCAGACCCCTCGGTTCTTCCCAGNAGACTAAGACAAGGGGAGCTGAACTCACGCCTGACC
  TCCGATACCAACCTGATGAAGTAACTGGCTTCCTTTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000544 unedited
  ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTATTTTAAAATATATGTA
  TTATGCCACAAAATACTCCTCATCACCAGGCAAAGCTCTAGTCACCAGGGAATTAAGTTT
  CCTGGACACAGACAGCCCCCACCCCACCCCACCCCACCTTTCCACCCCACCAAAAGCACA
  CAGTGTCCAAATCTCCATCATGCCTGCAACTCAGGAACAGCTATCTGGCCGCACAGCTCT
  AGGGAAACTCAAAGCAGGAACAGCTCTGGGTCCTGGAGACGCCCCTGAGAAGAGGGCCCA
  GTATCCCTGGGGCCTCAGTCCATCAGCCGCTGCTGCACCAGGCGGGAATAAAGGTCCTGT
  CCCTCCTAGAGCTGGGCAAGCTTTCTGCAGCTTGCCCTCCTGGAACACCCAGGATCTGGT
  GGGCGCGCTGAACTGTCTGCAGCCTGTGAGCAATCACCACCACTGTGCGATCCCCACGGG
  AATTCCATTCCTGCAGGGCCTGCTTTCACTTGCCATTTAGGCACTAGTTCCCCTCTCTCA
  CTATGATGACCCTTCGGTTTCTCCATGGCCCGCGCCTTGTCCATACTTTGTTTTTGTCCC
  GGCAATACATTGGCTCCCCTTCTCCCGACCTCTGGGGTATTCCCATGCCCCCATTCCCGC
  ATGAAAAATCGGCGTGGACATCTGGCCAGCGCCTCACCTTTTATTCCTGTAATCTTCCTA
  CCCATAACATCGTTTTTTCTACAATAACTCGTTATAATATATGTTTTTGCCAACTTAAAC
  CCTCGGTTCTGCGCTTCCACGCTATTCCCGTNCATGGCTCTTGCCCCTCTCCCCTCGTCC
  TCTTTGTCCTCTCATCTCCTCCCACAGCTACCCTTTGTTTTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000544
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000544.3, NP_000535.3
• RefSeq Size: 5679 bp
• RefSeq ORF: 2112 bp
• Locus ID: 6891
• UniProt ID: Q03519
• Cytogenetics: 6p21.32
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters, Antigen processing and presentation, Primary immunodeficiency
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1, B allele) represents the longer transcript and encodes the longest isoform (1). An allele (variant 1, A allele) exists in which a single nt change creates an internal stop codon, leading to a protein that is 17 aa shorter at the C-terminus.