WNT4 (NM_030761) Human Untagged Clone

CAT#: SC109791

WNT4 (untagged)-Human wingless-type MMTV integration site family, member 4 (WNT4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WNT4
• Synonyms: SERKAL; WNT-4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_030761 edited
  ATGAGTCCCCGCTCGTGCCTGCGTTCGCTGCGCCTCCTCGTCTTCGCCGTCTTCTCAGCC
  GCCGCGAGCAACTGGCTGTACCTGGCCAAGCTGTCGTCGGTGGGGAGCATCTCAGAGGAG
  GAGACGTGCGAGAAACTCAAGGGCCTGATCCAGAGGCAGGTGCAGATGTGCAAGCGGAAC
  CTGGAAGTCATGGACTCGGTGCGCCGCGGTGCCCAGCTGGCCATTGAGGAGTGCCAGTAC
  CAGTTCCGGAACCGGCGCTGGAACTGCTCCACACTCGACTCCTTGCCCGTCTTCGGCAAG
  GTGGTGACGCAAGGGACTCGGGAGGCGGCCTTCGTGTACGCCATCTCTTCGGCAGGTGTG
  GCCTTTGCAGTGACGCGGGCGTGCAGCAGTGGGGAGCTGGAGAAGTGCGGCTGTGACAGG
  ACAGTGCATGGGGTCAGCCCACAGGGCTTCCAGTGGTCAGGATGCTCTGACAACATCGCC
  TACGGTGTGGCCTTCTCACAGTCGTTTGTGGATGTGCGGGAGAGAAGCAAGGGGGCCTCG
  TCCAGCAGAGCCCTCATGAACCTCCACAACAATGAGGCCGGCAGGAAGGCCATCCTGACA
  CACATGCGGGTGGAATGCAAGTGCCACGGGGTGTCAGGCTCCTGTGAGGTAAAGACGTGC
  TGGCGAGCCGTGCCGCCCTTCCGCCAGGTGGGTCACGCACTGAAGGAGAAGTTTGATGGT
  GCCACTGAGGTGGAGCCACGCCGCGTGGGCTCCTCCAGGGCACTGGTGCCACGCAACGCA
  CAGTTCAAGCCGCACACAGATGAGGACCTGGTGTACTTGGAGCCTAGCCCCGACTTCTGT
  GAGCAGGACATGCGCAGCGGCGTGCTGGGCACGAGGGGCCGCACATGCAACAAGACGTCC
  AAGGCCATCGACGGCTGTGAGCTGCTGTGCTGTGGCCGCGGCTTCCACACGGCGCAGGTG
  GAGCTGGCTGAACGCTGCAGCTGCAAATTCCACTGGTGCTGCTTCGTCAAGTGCCGGCAG
  TGCCAGCGGCTCGTGGAGTTGCACACGTGCCGATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030761 unedited
  NNGGTTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGTCTGG
  TCCGCGCCGGGCAGCGGGCGCAGCAGCGGGCAGGCTGCCGGCAGGCACACGGAGGCAGAG
  CCCCGCCGCGCGCGCCCCGGCCCGCCCGCGGGCGCCCACCTGCAGCCCCGACGGGAGGCC
  CCCCGCGGCCGCAGCCGCTGCCCCGGGCCGGGCGCCCGCGGCGGCACCATGAGTCCCCGC
  TCGTGCCTGCGTTCGCTGCGCCTCCTCGTCTTCGCCGTCTTCTCAGCCGCCGCGAGCAAC
  TGGCTGTACCTGGCCAAGCTGTCGTCGGTGGGGAGCATCTCAGAGGAGGAGACGTGCGAG
  AAACTCAAGGGCCTGATCCAGAGGCAGGTGCAGATGTGCAAGCGGAACCTGGAAGTCATG
  GACTCGGTGCGCCGCGGTGCCCAGCTGGCCATTGAGGAGTGCCAGTACCAGTTCCGGAAC
  CGGCGCTGGAACTGCTCCACACTCGACTCCTTGCCCGTCTTCGGCAAGGTGGTGACGCAA
  GGGACTCGGGAGGCGGCCTTCGTGTACGCCATCTCTTCGGCAGGTGTGGCCTTTGCAGTG
  ACGCGGGCGTGCAGCAGTGGGGAGCTGGAGAAGTGCGGCTGTGACAGGACAGTGCATGGG
  GTCAGCCCACAGGGCTTCCAGGGGTCAGGATGCTCTGACACATCGCCTACGGTGTGGCCT
  TTCTACAGTCTTTGTGGATGTGCCGGAAAGAGCAAGGGGGCCTCGTCCAGCAAAGCCCTC
  ATGAACCCTCACACCATGAGGCCGGCAGAAGGCCTCCTGACACACATGCGGTGGATGGAA
  GGCCACGGGGTGGAAGCTCCTGTAGGAAAAAACGGCTGGGAAGCCCGCCGCCCTTCGCCA
  GGGGGTACCCCCTGAAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030761
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030761.3, NP_110388.2
• RefSeq Size: 1595 bp
• RefSeq ORF: 1056 bp
• Locus ID: 54361
• UniProt ID: P56705
• Cytogenetics: 1p36.12
• Domains: wnt
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]