PML Protein (PML) (NM_033240) Human Untagged Clone

CAT#: SC109584

PML (untagged)-Human promyelocytic leukemia (PML), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PML Protein
• Synonyms: MYL; PP8675; RNF71; TRIM19
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033240 edited
  GAATTCGGCACGAGGCTAAACCGAGAATCGAAACTAAGCTGGGGTCCATGGAGCCTGCAC
  CCGCCCGATCTCCGAGGCCCCAGCAGGACCCCGCCCGGCCCCAGGAGCCCACCATGCCTC
  CCCCCGAGACCCCCTCTGAAGGCCGCCAGCCCAGCCCCAGCCCCAGCCCTACAGAGCGAG
  CCCCCGCTTCGGAGGAGGAGTTCCAGTTTCTGCGCTGCCAGCAATGCCAGGCGGAAGCCA
  AGTGCCCGAAGCTGCTGCCTTGTCTGCACACGCTGTGCTCAGGATGCCTGGAGGCGTCGG
  GCATGCAGTGCCCCATCTGCCAGGCGCCCTGGCCCCTAGGTGCAGACACACCCGCCCTGG
  ATAACGTCTTTTTCGAGAGTCTGCAGCGGCGCCTGTCGGTGTACCGGCAGATTGTGGATG
  CGCAGGCTGTGTGCACCCGCTGCAAAGAGTCGGCCGACTTCTGGTGCTTTGAGTGCGAGC
  AGCTCCTCTGCGCCAAGTGCTTCGAGGCACACCAGTGGTTCCTCAAGCACGAGGCCCGGC
  CCCTAGCAGAGCTGCGCAACCAGTCGGTGCGTGAGTTCCTGGACGGCACCCGCAAGACCA
  ACAACATCTTCTGCTCCAACCCCAACCACCGCACCCCTACGCTGACCAGCATCTACTGCC
  GAGGATGTTCCAAGCCGCTGTGCTGCTCGTGCGCGCTCCTTGACAGCAGCCACAGTGAGC
  TCAAGTGCGACATCAGCGCAGAGATCCAGCAGCGACAGGAGGAGCTGGACGCCATGACGC
  AGGCGCTGCAGGAGCAGGATAGTGCCTTTGGCGCGGTTCACGCGCAGATGCACGCGGCCG
  TCGGCCAGCTGGGCCGCGCGCGTGCCGAGACCGAGGAGCTGATCCGCGAGCGCGTGCGCC
  AGGTGGTAGCTCACGTGCGGGCTCAGGAGCGCGAGCTGCTGGAGGCTGTGGACGCGCGGT
  ACCAGCGCGACTACGAGGAGATGGCCAGTCGGCTGGGCCGCCTGGATGCTGTGCTGCAGC
  GCATCCGCACGGGCAGCGCGCTGGTGCAGAGGATGAAGTGCTACGCCTCGGACCAGGAGG
  TGCTGGACATGCACGGTTTCCTGCGCCAGGCGCTCTGCCGCCTGCGCCAGGAGGAGCCCC
  AGAGCCTGCAAGCTGCCGTGCGCACCGATGGCTTCGACGAGTTCAAGGTGCGCCTGCAGG
  ACCTCAGCTCTTGCATCACCCAGGGGAAAGATGCAGCTGTATCCAAGAAAGCCAGCCCAG
  AGGCTGCCAGCACTCCCAGGGACCCTATTGACGTTGACCTGGATGTCTCCAATACAACGA
  CAGCCCAGAAGAGGAAGTGCAGCCAGACCCAGTGCCCCAGGAAGGTCATCAAGATGGAGT
  CTGAGGAGGGGAAGGAGGCAAGGTTGGCTCGGAGCTCCCCGGAGCAGCCCAGGCCCAGCA
  CCTCCAAGGCAGTCTCACCACCCCACCTGGATGGACCGCCTAGCCCCAGGAGCCCCGTCA
  TAGGAAGTGAGGTCTTCCTGCCCAACAGCAACCACGTGGCCAGTGGCGCCGGGGAGGCAG
  AGGAACGCGTTGTGGTGATCAGCAGCTCGGAAGACTCAGATGCCGAAAACTCGGTGAGTG
  GCCCAGAAGTTCAGCCCAGGACTCCTGCCTCCCCCCATTTCAGGTCCCAGGGGGCACAGC
  CACAGCAGGTGACTCTCAGACTTGCCTTGCGCCTGGGGAATTTTCCAGTGAGGCATTGAG
  TCCCAAGCTGTGCTGAGGACAGTCTCCAAATGACAGCTGCATGCCTGGACCACCCCAGCC
  CTCCCACTACACCAGGGCCAGGAGCTCTTCTGAAATGCTGATAGCATGTGTCCAGAGCCC
  TGTCTCTTTTCTGGAATGTCCAAGACCTTTGTCTGGAGCTTCCTTATGCATTTTCTGTCC
  TCTAAGTCCTCAGTGAGGAGGGCTGGACAAGAATCCATTCCTTGGTTTATTACAGCCAGT
  GGGGGCCAGTGAAGGGGTGTCAGGCCACAGGGCAGCTATATAGGGGCCAAACAAGTGAGG
  TTTGACTCCATCCATGTAGAAAGATATATAAATCCATTCCACAGTGAAACAGGTGGCCTC
  GTGGGTAGTGACCCTTCTGTCCCTAGAGGTTTATTACTAGAGGCTGGACTATCACCTGTC
  CAGGGGAAAAGGGGATCTGAATAGAGTGAAAGGTTGGACTGGGTGGCCCCTGAGGTCTCT
  TCCAGCCCTCAGTCTGAGGTTCTGTATTGGAAAGTGCATGGAGCCCATGGAGACCGCCGA
  GCCACAGTCCTCGCCAGCCCACTCCTCGCCAGCCCACTCCTCGCCAGCCCACTCCTCGCC
  AGTCCAGTCTCTGCTGAGAGCACAAGGAGCCTCCAGCCTGCCCTGTGGCACATACCACCC
  CCCAGCTTGGCCTCCCCACCAGCCCGCTGAGCAGGCTGCCACCCCCGATGCTGAGCCTCA
  CAGCGAGCCTCCTGATCACCAGGAGCGCCCTGCCGTCCACCGTGGGATCCGCTACCTGTT
  GTACAGAGCACAGAGAGCCATCCGCCTTCGCCATGCCCTCCGCTTGCACCCTCAATTGCA
  TCGGGCCCCTATTCGGACTTGGTCTCCCCATGTGGTCCAAGCCAGCACTCCTGCCATCAC
  AGGGCCCCTCAACCATCCTGCCAATGCCCAGGAACATCCTGCCCAGCTGCAAAGGGGCAT
  CAGCCCACCCCACCGGATACGAGGGGCTGTGCGATCCCGCAGCCGCTCCCTCCGGGGCTC
  CTCCCATTTATCCCAGTGGCTCAACAACTTTTTTGCCCTCCCCTTCTCCTCCATGGCTTC
  CCAGCTTGACATGTCTTCCGTGGTGGGGGCAGGGGAAAGCAGAGCCCAGACTCTTGGAGC
  AGGTGTTCCCCCTGGGGACTCTGTCAGAGGCTCCATGGAGGCCTCTCAAGTCCAAGTGCC
  TCTGGAAGCCTCTCCAATTACATTCCCACCACCCTGTGCCCCAGAAAGGCCCCCCATCAG
  CCCAGTCCCAGGCGCCCGTCAAGCAGGCCTCTGAGAGTGCTACCCTTCTCTTGTAACCTT
  GCAGCCAACACCCCTGCCCGGCCCCTGAGCTGCCTCCTCCAGCCCATGCTCTTACAGGCC
  CTGCACAGAGTAGCACTCATTAATTCTTGGTTAAGGAATGAATCAACGAATGAATGGCTA
  TGCATGGACCTCTGGGCAGGGAGACCTGGGTCTTCTCTGGCTGAGAGGGGAAGGCTAAGG
  CATGGCTGAGATTCAAGCCACCATTCCAGGCCTCTTTGCCCAAGAAAGAAACTTCTGTCA
  CCCTTGCACTCTCCTGTATTCTGAGTCCCTGGCCAATAGCACAGCCTTCCATGCCCCGAC
  CCCCACCCCAAGCCTCTCCACTAGGCCTCTGCCAGGATCTAAGCCCATGAGCACAGGGAC
  TGGCTATCCCAAGACCTGGCAGATGTGGCTGCTCAATAAACACTTGTTGAACCATCAAAA
  AAAAAAAAAAAAAACTCGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033240
• Insert Size: 3630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033240.2, NP_150243.2
• RefSeq Size: 3751 bp
• RefSeq ORF: 1836 bp
• Locus ID: 5371
• UniProt ID: P29590
• Cytogenetics: 15q24.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer, Ubiquitin mediated proteolysis
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 3' UTR and the 3' coding region compared to variant 1. The resulting isoform (2, also known PML-2, PML-V and TRIM19beta) contains a distinct C-terminus compared to isoform 1.