PLAT (NM_033011) Human Untagged Clone

CAT#: SC109578

PLAT (untagged)-Human plasminogen activator, tissue (PLAT), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLAT
• Synonyms: T-PA; TPA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033011 edited
  GAATTCGGCACGAGGCAGGAGTCCAGGGCTGGAGAGAAAACCTCTGCGAGGAAAGGGAAG
  GAGCAAGCCGTGAATTTAAGGGACGCTGTGAAGCAATCATGGATGCAATGAAGAGAGGGC
  TCTGCTGTGTGCTGCTGCTGTGTGGAGCAGTCTTCGTTTCGCCCAGCCAGGAAATCCATG
  CCCGATTCAGAAGAGGAGCCAGATCTTACCAAGGTTGCAGCGAGCCAAGGTGTTTCAACG
  GGGGCACCTGCCAGCAGGCCCTGTACTTCTCAGATTTCGTGTGCCAGTGCCCCGAAGGAT
  TTGCTGGGAAGTGCTGTGAAATAGATACCAGGGCCACGTGCTACGAGGACCAGGGCATCA
  GCTACAGGGGCACGTGGAGCACAGCGGAGAGTGGCGCCGAGTGCACCAACTGGAACAGCA
  GCGCGTTGGCCCAGAAGCCCTACAGCGGGCGGAGGCCAGATGCCATCAGGCTGGGCCTGG
  GGAACCACAACTACTGCAGAAACCCAGATCGAGACTCAAAGCCCTGGTGCTACGTCTTTA
  AGGCGGGGAAGTACAGCTCAGAGTTCTGCAGCACCCCTGCCTGCTCTGAGGGAAACAGTG
  ACTGCTACTTTGGGAATGGGTCAGCCTACCGTGGCACGCACAGCCTCACCGAGTCGGGTG
  CCTCCTGCCTCCCGTGGAATTCCATGATCCTGATAGGCAAGGTTTACACAGCACAGAACC
  CCAGTGCCCAGGCACTGGGCCTGGGCAAACATAATTACTGCCGGAATCCTGATGGGGATG
  CCAAGCCCTGGTGCCACGTGCTGAAGAACCGCAGGCTGACGTGGGAGTACTGTGATGTGC
  CCTCCTGCTCCACCTGCGGCCTGAGACAGTACAGCCAGCCTCAGTTTCGCATCAAAGGAG
  GGCTCTTCGCCGACATCGCCTCCCACCCCTGGCAGGCTGCCATCTTTGCCAAGCACAGGA
  GGTCGCCCGGAGAGCGGTTCCTGTGCGGGGGCATACTCATCAGCTCCTGCTGGATTCTCT
  CTGCCGCCCACTGCTTCCAGGAGAGGTTTCCGCCCCACCACCTGACGGTGATCTTGGGCA
  GAACATACCGGGTGGTCCCTGGCGAGGAGGAGCAGAAATTTGAAGTCGAAAAATACATTG
  TCCATAAGGAATTCGATGATGACACTTACGACAATGACATTGCGCTGCTGCAGCTGAAAT
  CGGATTCGTCCCGCTGTGCCCAGGAGAGCAGCGTGGTCCGCACTGTGTGCCTTCCCCCGG
  CGGACCTGCAGCTGCCGGACTGGACGGAGTGTGAGCTCTCCGGCTACGGCAAGCATGAGG
  CCTTGTCTCCTTTCTATTCGGAGCGGCTGAAGGAGGCTCATGTCAGACTGTACCCATCCA
  GCCGCTGCACATCACAACATTTACTTAACAGAACAGTCACCGACAACATGCTGTGTGCTG
  GAGACACTCGGAGCGGCGGGCCCCAGGCAAACTTGCACGACGCCTGCCAGGGCGATTCGG
  GAGGCCCCCTGGTGTGTCTGAACGATGGCCGCATGACTTTGGTGGGCATCATCAGCTGGG
  GCCTGGGCTGTGGACAGAAGGATGTCCCGGGTGTGTACACCAAGGTTACCAACTACCTAG
  ACTGGATTCGTGACAACATGCGACCGTGCCCAGGAACACCCGACTCCTCAAAAGCAAATG
  AGATCCCGCCTCTTCTTCTTCAGAAGACACTGCAAAGGCGCAGTGCTTCTCTACAGACTT
  CTCCAGACCCACCACACCGCAGAAGCGGGACGAGACCCTACAGGAGAGGGAAGAGTGCAT
  TTTCCCAGATACTTCCCATTTTGGAAGTTTTCAGGACTTGGTCTGATTTCAGGATACTCT
  GTCAGATGGGAAGACATGAATGCACACTAGCCTCTCCAGGAATGCCTCCTCCCTGGGCAG
  AAAGTGGCCATGCCACCCTGTTTTCAGCTAAAGCCCAACCTCCTGACCTGTCACCGTGAG
  CAGCTTTGGAAACAGGACCACAAAAATGAAAGCATGTCTCAATAGTAAAAGATAACAAGA
  TCTTTCAGGAAAGACGGATTGCATTAGAAATAGACAGTATATTTATAGTCACAAGAGCCC
  AGCAGGGCCTCAAAGTTGGGGCAGGCTGGCTGGCCCGTCATGTTCCTCAAAAGCACCCTT
  GACGTCAAGTCTCCTTCCCCTTTCCCCACTCCCTGGCTCTCAGAAGGTATTCCTTTTGTG
  TACAGTGTGTAAAGTGTAAATCCTTTTTCTTTATAAACTTTAGAGTAGCATGAGAGAATT
  GTATCATTTGAACAACTAGGCTTCAGCATATTTATAGCAATCCATGTTAGTTTTTACTTT
  CTGTTGCCACAACCCTGTTTTATACTGTACTTAATAAATTCAGATATATTTTTCACAGTT
  TTTCCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033011 unedited
  AAAAACCTTCGGCACGAGGCAGGAGTCCATTGCTGGAGAGAAAACCTCTGCGAGGAAAGG
  GAAGGAGCAAGCCGTGAATTTAAGGGACGCTGTGAAGCAATCATGGATGCAATGAAGAGA
  GGGCTCTGCTGTGTGCTGCTGCTGTGTGGAGCAGTCTTCGTTTCGCCCAGCCAGGAAATC
  CCCTCCCGATTCAGAAGATGAGCCTGATCTTACCAAGGTTGCAGCGAGCCAAGGTGTTTC
  AACGGGGGCACCTGCCAGCAGGCCCTGTACTTCTCAGATTTCGTGTGCCAGTGCCCCGAA
  GGATTTGCTGGGAAGTGCTGTGAAATAGATACCAGGGCCACGTGCTACGAGGACCAGGGC
  ATCAGCTACAGGGGCACGTGGAGCACAGCGGAGAGTGGCGCCGAGTGCACCAACTGGAAC
  AGCAGCGCGTTGGCCCAGAAGCCCTACAGCGGGCGGAGGCCAGATGCCATCAGGCTGGGC
  CTGGGGAACCACAACTACTGCAGAAACCCAGATCGAGACTCAAAGCCCTGGTGCTACGTC
  TTTAATGCGGGGAAGTACAGCTCAGAGTTCTGCAGCACCCCTGCCTGCTCTGAGGGAAAC
  AGTGACTGCTACTTTGGGAATGGGTCAGCCTACCGTGGCACGCACAGCCCTCCCGAGTCG
  GGTGCCTCCTGGCTCCCGTGGAATTCCCTGATCCTGATAGGCATTGTTTACACAGCACAG
  AACCCCAGTGCCCATGCACTTGGGCCTGGGGCAACCTAATTACTGGCCGGATTCCTGGTG
  GGGATGGCCCAGCCCTGGTGCCACTTGCTGGAATACCCGCGGCTGGACGTGGGAGGTCCT
  GGGATGGGCCCTCCTGCTCTCCCTGGGGGCCGGGGACAGTCCAGCCAGCCTCATTTTTCA
  TAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033011 unedited
  NNTTTTAGCTATGNACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGG
  AAAAACTGTGAAAAATATATCTGAATTTATTAAGTACAGTATAAAACAGGGTTGTGGCAA
  CAGAAAGTAAAAACTAACATGGATTGCTATAAATATGCTGAAGCCTAGTTGTTCAAATGA
  TACAATTCTCTCATGCTACTCTAAAGTTTATAAAGAAAAAGGATTTACACTTTACACACT
  GTACACAAAAGGAATACCTTCTGAGAGCCAGGGAGTGGGGAAAGGGGAAGGAGACTTGAC
  GTCAAGGGTGCTTTTGAGGAACATGACGGGCCAGCCAGCCTGCCCCAACTTTGAGGCCCT
  GCTGGGCTCTTGTGACTATAAATATACTGTCTATTTCTAATGCAATCCGTCTTTCCTGAA
  AGATCTTGTTATCTTTTACTATTGAGACATGCTTTCATTTTTGTGGTCCTGTTTCCAAAG
  CTGCTCACGGTGACAGGTCAGGAGGTTGGGCTTTAGCTGAAAACAGGGTGGCATGGCCAC
  TTTCTGCCCAGGGAGGAGGCATTCCTGGAGAGGCTAGTGTGCATTCATGTCTTCCCATCT
  GACAGAGTATCCTGAAATCAGACCAAGTCCTGAAAACTTCCAAAATGGGAAGTATCTGGG
  AAAATGCACTCTTCCCTCTCCTGTAGGGTCTCGTCCCGCTTCTGNCGTGTGGTGGGTCTG
  GAGAAGTCTGTAAAGAGCACTGCGCCTTTGCAGTGTCTTCTGAAGAAGAAGAGGCGGATC
  TCATTTGCTTTTTGAGAGTCGGGTGTTTCTGGGCACGGTCGCATGNTGTCACGAATCCAG
  TCTAGGTAGTTGGGTACCCTGGTGTACACACCCGGNACATNCTTNTGTCCACANCCCCAG
  CCCAGCTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033011
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033011.1, NP_127509.1
• RefSeq Size: 2534 bp
• RefSeq ORF: 1551 bp
• Locus ID: 5327
• UniProt ID: P00750
• Cytogenetics: 8p11.21
• Domains: KR, Tryp_SPc, EGF
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1. This isoform (3) may undergo proteolytic processing similar to isoform 1.