PAX6 (NM_001604) Human Untagged Clone

CAT#: SC109551

PAX6 (untagged)-Human paired box 6 (PAX6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAX6
• Synonyms: AN; AN1; AN2; ASGD5; D11S812E; FVH1; MGDA; WAGR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109551 sequence for NM_001604 edited (data generated by NextGen Sequencing)
  ATGCAGAACAGTCACAGCGGAGTGAATCAGCTCGGTGGTGTCTTTGTCAACGGGCGGCCA
  CTGCCGGACTCCACCCGGCAGAAGATTGTAGAGCTAGCTCACAGCGGGGCCCGGCCGTGC
  GACATTTCCCGAATTCTGCAGACCCATGCAGATGCAAAAGTCCAAGTGCTGGACAATCAA
  AACGTGTCCAACGGATGTGTGAGTAAAATTCTGGGCAGGTATTACGAGACTGGCTCCATC
  AGACCCAGGGCAATCGGTGGTAGTAAACCGAGAGTAGCGACTCCAGAAGTTGTAAGCAAA
  ATAGCCCAGTATAAGCGGGAGTGCCCGTCCATCTTTGCTTGGGAAATCCGAGACAGATTA
  CTGTCCGAGGGGGTCTGTACCAACGATAACATACCAAGCGTGTCATCAATAAACAGAGTT
  CTTCGCAACCTGGCTAGCGAAAAGCAACAGATGGGCGCAGACGGCATGTATGATAAACTA
  AGGATGTTGAACGGGCAGACCGGAAGCTGGGGCACCCGCCCTGGTTGGTATCCGGGGACT
  TCGGTGCCAGGGCAACCTACGCAAGATGGCTGCCAGCAACAGGAAGGAGGGGGAGAGAAT
  ACCAACTCCATCAGTTCCAACGGAGAAGATTCAGATGAGGCTCAAATGCGACTTCAGCTG
  AAGCGGAAGCTGCAAAGAAATAGAACATCCTTTACCCAAGAGCAAATTGAGGCCCTGGAG
  AAAGAGTTTGAGAGAACCCATTATCCAGATGTGTTTGCCCGAGAAAGACTAGCAGCCAAA
  ATAGATCTACCTGAAGCAAGAATACAGGTATGGTTTTCTAATCGAAGGGCCAAATGGAGA
  AGAGAAGAAAAACTGAGGAATCAGAGAAGACAGGCCAGCAACACACCTAGTCATATTCCT
  ATCAGCAGTAGTTTCAGCACCAGTGTCTACCAACCAATTCCACAACCCACCACACCGGTT
  TCCTCCTTCACATCTGGCTCCATGTTGGGCCGAACAGACACAGCCCTCACAAACACCTAC
  AGCGCTCTGCCGCCTATGCCCAGCTTCACCATGGCAAATAACCTGCCTATGCAACCCCCA
  GTCCCCAGCCAGACCTCCTCATACTCCTGCATGCTGCCCACCAGCCCTTCGGTGAATGGG
  CGGAGTTATGATACCTACACCCCCCCACATATGCAGACACACATGAACAGTCAGCCAATG
  GGCACCTCGGGCACCACTTCAACAGGACTCATTTCCCCTGGTGTGTCAGTTCCAGTTCAA
  GTTCCCGGAAGTGAACCTGATATGTCTCAATACTGGCCAAGATTACAGTAA
  
  Clone variation with respect to NM_001604.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001604 unedited
  GTCACAATTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGCCTCGTGCC
  GAATTCGGCACGAGGCACACACCAACCCAGCAACATCCGTGGAGAAAACTCTCACCAGCA
  ACTCCTTTAAAACACCGTCATTTCAAACCATTGTGGTCTTCAAGCAACAACAGCAGCACA
  AAAAACCCCAACCAAACAAAACTCTTGACAGAAGCTGTGACAACCAGAAAGGATGCCTCA
  TAAAGGGGGAAGACTTTAACTAGGGGCGCGCAGATGTGTGAGGCCTTTTATTGTGAGAGT
  GGACAGACATCCGAGATTTCAGAGCCCCATATTCGAGCCCCGTGGAATCCCGCGGCCCCC
  AGCCAGAGCCAGCATGCAGAACAGTCACAGCGGAGTGAATCAGCTCGGTGGTGTCTTTGT
  CAACGGGCGGCCACTGCCGGACTCCACCCGGCAGAAGATTGTAGAGCTAGCTCACAGCGG
  GGCCCGGCCGTGCGACATTTCCCGAATTCTGCAGACCCATGCAGATGCAAAAGTCCAAGT
  GCTGGACAATCAAAACGTGTCCAACGGATGTGTGAGTAAAATTCTGGGCAGGTATTACGA
  GACTGGCTCCATCAGACCCAGGGCAATCGGTGGTAGTAAACCGAGAGTAGCGACTCCAGA
  AGTTGTAAGCAAAATAGCCCAGTATAAGCGGGAGTGCCCGTCCATCTTTGCTTGGGAAAT
  CCGAGACAGATTACTGTCCCGAGGGGTCTGTACCAACGATAACATACCAAGCGTGTCATC
  AATAAAACAGAGTCTTCGCACCCTGGCTAGCGAAAAGCACCAGATGGGCCCAGACCGGAT
  GTATGATTAACTAAAGATGTTGAACGGGCAAACCCGAAGCTGGGGCACCCGCCCTGGTTG
  GTATCCGGGGGACTTCGGTGCCACGGCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001604 unedited
  CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTACTGTAATCTTG
  GCCAGTATTGAGACATATCAGGTTCACTTCCGGGAACTTGAACTGGAACTGACACACCAG
  GGGAAAATGAGTCCTGTTGAAGTGGTGCCCGAGGTGCCCATTGGCTGACTGTTCATGTGT
  GTCTGCATATGTGGGGGGGTGTAGGTATCATAACTCCGCCCATTCACCGAAGGGCTGGTG
  GGCAGCATGCAGGAGTATGAGGAGGTCTGGCTGGGGACTGGGGGTTGCATAGGCAGGTTA
  TTTGCCATGGTGAAGCTGGGCATAGGCGGCAGAGCGCTGTAGGTGTTTGTGAGGGCTGTG
  TCTGTTCGGCCCAACATGGAGCCAGATGTGAAGGAGGAAACCGGTGTGGTGGGTTGTGGA
  ATTGGTTGGTAGACACTGGTGCTGAAACTACTGCTGATAGGAATATGACTAGGTGTGTTG
  CTGGCCTGTCTTCTCTGATTCCTCAGTTTTTCTTCTCTTTTCCATTTGGCCCTTCGATTA
  GAAAACCATACCTGTATTCTTGCTTCAGGTAGATCTATTTTGGCTGCTAGTCTTTCTCGG
  GCAAACACATCTGGATAATGGGTTCTCTCAAACTCTTTTTCCAGGGCCTTAATTTGCTCT
  TGGTAAAGGATGTTCTATTTTTTTGCAGCTTCCGCTTCAGCTGAAATCGCATTTGAGCCT
  AATCTGAATCTTCTCCGCTTGAACTGATGGGATTTGTTATTCTTTCCCCCTTCTTTCTTG
  TTGCTGGCAGCCTTCTTGCGTAAGGTTCGCCTGGGCACCGAAATCCCCCGTAAACCAACC
  ACGGGCGGGTTGCCCCAGCTTTCGGGTCTGCCCCGTCAACAATCCTTTATTTTTATAACT
  GCCGTGTGCGCCCTTTTGTGGTTTTTCCTTACCAGTTGCCAACAATCTTTTTATTGTTTA
  ACCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001604
• Insert Size: 1710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001604.3, NP_001595.2
• RefSeq Size: 2781 bp
• RefSeq ORF: 1311 bp
• Locus ID: 5080
• UniProt ID: P26367
• Cytogenetics: 11p13
• Domains: homeobox, PAX
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: This gene encodes paired box protein Pax-6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to a conserved paired box domain, a hallmark feature of this gene family, the encoded protein also contains a homeobox domain. Both domains are known to bind DNA and function as regulators of gene transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing results in multiple transcript variants encoding different isoforms. Interestingly, inclusion of a particular alternate coding exon has been shown to increase the length of the paired box domain and alter its DNA binding specificity. Consequently, isoforms that carry the shorter paired box domain regulate a different set of genes compared to the isoforms carrying the longer paired box domain. [provided by RefSeq, Mar 2019]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, and includes an alternate in-frame exon in the 5' coding region, compared to variant 1. It initiates from the B (P1) promoter. The encoded isoform (b, also known as 5a) is longer than isoform a. Variants 2, 4, 5, 8, and 17-19 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.