NFAT2 (NFATC1) (NM_172388) Human Untagged Clone

CAT#: SC109490

NFATC1 (untagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATC1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFAT2
• Synonyms: NF-ATC; NF-ATc1.2; NFAT2; NFATc
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_172388, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCAGCTTTTCATTGGGACGGCGGACGACCGCCTGCTGCGCCCGCACGCCTTCTACCAGGTGCACC
  GCATCACAGGGAAGACCGTGTCCACCACCAGCCACGAGGCCATCCTCTCCAACACCAAAGTCCTGGAGAT
  CCCACTCCTGCCGGAGAACAGCATGCGAGCCGTCATTGACTGTGCCGGAATCCTGAAACTCAGAAACTCC
  GACATTGAACTTCGGAAAGGAGAGACGGACATCGGGAGGAAGAACACACGGGTACGGCTGGTGTTCCGCG
  TTCACGTCCCGCAACCCAGCGGCCGCACGCTGTCCCTGCAGGTGGCCTCCAACCCCATCGAATGCTCCCA
  GCGCTCAGCTCAGGAGCTGCCTCTGGTGGAGAAGCAGAGCACGGACAGCTATCCGGTCGTGGGCGGGAAG
  AAGATGGTCCTGTCTGGCCACAACTTCCTGCAGGACTCCAAGGTCATTTTCGTGGAGAAAGCCCCAGATG
  GCCACCATGTCTGGGAGATGGAAGCGAAAACTGACCGGGACCTGTGCAAGCCGAATTCTCTGGTGGTTGA
  GATCCCGCCATTTCGGAATCAGAGGATAACCAGCCCCGTTCACGTCAGTTTCTACGTCTGCAACGGGAAG
  AGAAAGCGAAGCCAGTACCAGCGTTTCACCTACCTTCCCGCCAACGTTCCAATTATAAAAACAGAACCCA
  CTGATGATTATGAGCCTGCTCCAACCTGTGGACCGGTGAGCCAGGGGTTAAGTCCTCTCCCAAGACCATA
  CTACAGCCAGCAGCTCGCGATGCCACCCGACCCCAGCTCCTGCCTCGTGGCCGGCTTCCCGCCCTGTCCG
  CAGAGAAGCACCCTGATGCCAGCGGCCCCTGGCGTGAGCCCCAAGCTCCACGACCTTTCTCCCGCTGCCT
  ACACCAAGGGCGTTGCCAGCCCGGGCCACTGTCACCTCGGACTCCCGCAGCCGGCCGGAGAGGCCCCCGC
  CGTCCAGGACGTGCCCAGGCCAGTGGCCACGCACCCCGGCTCGCCCGGGCAGCCACCCCCGGCCCTGCTG
  CCACAGCAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172388 unedited
  NGTTTCGGCATTTTNGTATACGATCTCACTATAGGGCGGCCGCGATTCGGCACGAGGAAC
  CGAACCCCTGGCGGCCGCGACCCCGGCTCCCGCCCCGGCCCCGGCCCCGGCCCGACCCGC
  CATGACGGGGCTGGAGGACCAGGAGTTCGACTTCGAGTTCCTCTTCGAGTTTAACCAGCG
  CGACGAGGGCGCCGCCGCGGCCGCCCCAGCCCGACCCTGCCCGCCCTGGACTGGCAGCTG
  CCGTCCCACTCAGGCCCGTATGAGCTTCGGATTGAGGTGCAGCCCAAGTCCCACCACCGA
  GCCCACTACGAGACGGAGGGCAGCCGGGGGGCCGTGAAGGCGTCGGCCGGAGGACACCCC
  ATCGTGCAGCTGCATGGCTACTTGGAGAATGAGCCGCTGATGCTGCAGCTTTTCATTGGG
  ACGGCGGACGACCGCCTGCTGCGCCCGCACGCCTTCTACCAGGTGCACCGCATCACAGGG
  AAGACCGTGTCCACCACCAGCCACGAGGCCATCCTCTCCAACACCAAAGTCCTGGAGATC
  CCACTCCTGCCGGAGAACAGCATGCGAGCCGTCATTGACTGTGCCGGAATCCTGAAACTC
  AGAAACTCCGACATTGAACTTCGGAAAGGAGAGACGGACATCGGGAGGAAGAACACACGG
  GTACGGCTGGTGTTCCGCGTTCACGTCCCGCAACCCAGCGGCCGCACGCTGTCCCTGCAG
  GTGGCCTCCAACCCCATCGAATGCTCCCAGCGCTCAGCTCAGGAGCTGCCTCTGGTGGAG
  AAGCAGAGCACGGACAGCTATCCGTTCGTGGGCGGNAAGAAGATGGCCCTGTCCGGCCAC
  ACTTTCTGCAGGACTCCAAGGTCATTTNCGTGGAGAAAGCCCAGATGGCCACCTGTCTGG
  AAGATGGAGCGAAACTGACCGGGCCTGTGCAAGCGCATTTCTGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_172388 unedited
  GTACCGCGCCGCAATCTAGTAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTACAGCAAA
  AAAAACGGCTTTATTGGATCTATTTCCTAACTACAAAGACAGCTGACACAGACATCAAAC
  GTTTCCTTTTCAATGACAGTCCCCTGAAAAAGGCTGCACGTGACTCCTACAGTGCCGGGT
  GCAGGGTACCCAGCCGCAGGGGGGACGCGGCCACACGTCTTCACGGTTACGTGCAGACCG
  ACGGGATGGCCTTCAGGTTGTTTCTTTCCGTGAGTGAAACACAAGAGACGCGATTGTGCC
  GGAGCGCACGGTACAGGCCGTTCCTTCTGCGGGAGCCCTTTTTCCATCAGCGACGTTCTG
  TTCAGGGACTTCACAGCCGAAGCCTCCACCCGCAGGGGCATTTTAGGCAGGTCCCATAGA
  GGGGCTTTATTTTCTAAGGCCCTAGTCCAGGCCCCCCCGGCTTCAATGAATAACACCTGC
  CTCCCGCGCTTCCGGACCACTAGATCGTTGGGAGTATTCTTGCCTGCTAACCCCCTCTTT
  GGCCTTAGTGCCCCCCATCGCCTCTACACCTCTACCGGAGCCTTGTCCCCTTCGCACCAC
  ACCCTCCCCCGTCCCCTCACCAATCCCGCGTCACCGGAATTCCCTACCCAATTCACCCCG
  TTCTGTCTTCTGGTCCCTCCAACCTGAGTCCCCCTGCCTCCTTCCTTTATCATGCTCCAC
  AAGTTCGGACCCCCTCGGTCTCCCCGATGCCTTCATTATTGTTATATTTCTCATGTTTAG
  TTCTCGCAACCTCCCGACCTCCAGCTACCGAGCTTCATGAGTTTCGCCTGTTACAGGGCA
  ACTACATCCCTACGCACCCCTCACCGCTCTATTGCCCACCCGCCGTTTTTCCTCGCCCAT
  TCGTCCTTCCCACCCGCTCCTATTATGTGCCCTACTCCCGCGCACCGGAGCTGGGTCCAC
  CGAGTATATGGCATCTCTTACTGACCATATCATTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_172388
• Insert Size: 3250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172388.1, NP_765976.1
• RefSeq Size: 3671 bp
• RefSeq ORF: 1062 bp
• Locus ID: 4772
• UniProt ID: O95644
• Cytogenetics: 18q23
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (4) lacks an internal exon in the 5' region, which results in a downstream translation start codon, and has an alternate splice site in the 3' coding region, compared to variant 6. The encoded isoform (D) is shorter at both the N- and C- termini, compared to isoform F.