L1CAM (NM_000425) Human Untagged Clone

CAT#: SC109376

L1CAM (untagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: L1CAM
• Synonyms: CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109376 sequence for NM_000425 edited (data generated by NextGen Sequencing)
  ATGGTCGTGGCGCTGCGGTACGTGTGGCCTCTCCTCCTCTGCAGCCCCTGCCTGCTTATC
  CAGATCCCCGAGGAATATGAAGGACACCATGTGATGGAGCCACCTGTCATCACGGAACAG
  TCTCCACGGCGCCTGGTTGTCTTCCCCACAGATGACATCAGCCTCAAGTGTGAGGCCAGT
  GGCAAGCCCGAAGTGCAGTTCCGCTGGACGAGGGATGGTGTCCACTTCAAACCCAAGGAA
  GAGCTGGGTGTGACCGTGTACCAGTCGCCCCACTCTGGCTCCTTCACCATCACGGGCAAC
  AACAGCAACTTTGCTCAGAGGTTCCAGGGCATCTACCGCTGCTTTGCCAGCAATAAGCTG
  GGCACCGCCATGTCCCATGAGATCCGGCTCATGGCCGAGGGTGCCCCCAAGTGGCCAAAG
  GAGACAGTGAAGCCCGTGGAGGTGGAGGAAGGGGAGTCAGTGGTTCTGCCTTGCAACCCT
  CCCCCAAGTGCAGAGCCTCTCCGGATCTACTGGATGAACAGCAAGATCTTGCACATCAAG
  CAGGACGAGCGGGTGACGATGGGCCAGAACGGCAACCTCTACTTTGCCAATGTGCTCACC
  TCCGACAACCACTCAGACTACATCTGCCACGCCCACTTCCCAGGCACCAGGACCATCATT
  CAGAAGGAACCCATTGACCTCCGGGTCAAGGCCACCAACAGCATGATTGACAGGAAGCCG
  CGCCTGCTCTTCCCCACCAACTCCAGCAGCCACCTGGTGGCCTTGCAGGGGCAGCCATTG
  GTCCTGGAGTGCATCGCCGAGGGCTTTCCCACGCCCACCATCAAATGGCTGCGCCCCAGT
  GGCCCCATGCCAGCCGACCGTGTCACCTACCAGAACCACAACAAGACCCTGCAGCTGCTG
  AAAGTGGGCGAGGAGGATGATGGCGAGTACCGCTGCCTGGCCGAGAACTCACTGGGCAGT
  GCCCGGCATGCGTACTATGTCACCGTGGAGGCTGCCCCGTACTGGCTGCACAAGCCCCAG
  AGCCATCTATATGGGCCAGGAGAGACTGCCCGCCTGGACTGCCAAGTCCAGGGCAGGCCC
  CAACCAGAGGTCACCTGGAGAATCAACGGGATCCCTGTGGAGGAGCTGGCCAAAGACCAG
  AAGTACCGGATTCAGCGTGGCGCCCTGATCCTGAGCAACGTGCAGCCCAGTGACACAATG
  GTGACCCAATGTGAGGCCCGCAACCGGCACGGGCTCTTGCTGGCCAATGCCTACATCTAC
  GTTGTCCAGCTGCCAGCCAAGATCCTGACTGCGGACAATCAGACGTACATGGCTGTCCAG
  GGCAGCACTGCCTACCTTCTGTGCAAGGCCTTCGGAGCGCCTGTGCCCAGTGTTCAGTGG
  CTGGACGAGGATGGGACAACAGTGCTTCAGGACGAACGCTTCTTCCCCTATGCCAATGGG
  ACCCTGGGCATTCGAGACCTCCAGGCCAATGACACCGGACGCTACTTCTGCCTGGCTGCC
  AATGACCAAAACAATGTTACCATCATGGCTAACCTGAAGGTTAAAGATGCAACTCAGATC
  ACTCAGGGGCCCCGCAGCACAATCGAGAAGAAAGGTTCCAGGGTGACCTTCACGTGCCAG
  GCCTCCTTTGACCCCTCCTTGCAGCCCAGCATCACCTGGCGTGGGGACGGTCGAGACCTC
  CAGGAGCTTGGGGACAGTGACAAGTACTTCATAGAGGATGGGCGCCTGGTCATCCACAGC
  CTGGACTACAGCGACCAGGGCAACTACAGCTGCGTGGCCAGTACCGAACTGGATGTGGTG
  GAGAGTAGGGCACAGCTCTTGGTGGTGGGGAGCCCTGGGCCGGTGCCACGGCTGGTGCTG
  TCCGACCTGCACCTGCTGACGCAGAGCCAGGTGCGCGTGTCCTGGAGTCCTGCAGAAGAC
  CACAATGCCCCCATTGAGAAATATGACATTGAATTTGAGGACAAGGAAATGGCGCCTGAA
  AAATGGTACAGTCTGGGCAAGGTTCCAGGGAACCAGACCTCTACCACCCTCAAGCTGTCG
  CCCTATGTCCACTACACCTTTAGGGTTACTGCCATAAACAAATATGGCCCCGGGGAGCCC
  AGCCCGGTCTCTGAGACTGTGGTCACACCTGAGGCAGCCCCAGAGAAGAACCCTGTGGAT
  GTGAAGGGGGAAGGAAATGAGACCACCAATATGGTCATCACGTGGAAGCCGCTCCGGTGG
  ATGGACTGGAACGCCCCCCAGGTTCAGTACCGCGTGCAGTGGCGCCCTCAGGGGACACGA
  GGGCCCTGGCAGGAGCAGATTGTCAGCGACCCCTTCCTGGTGGTGTCCAACACGTCCACC
  TTCGTGCCCTATGAGATCAAAGTCCAGGCCGTCAACAGCCAGGGCAAGGGACCAGAGCCC
  CAGGTCACTATCGGCTACTCTGGAGAGGACTACCCCCAGGCAATCCCTGAGCTGGAAGGC
  ATTGAAATCCTCAACTCAAGTGCCGTGCTGGTCAAGTGGCGGCCGGTGGACCTGGCCCAG
  GTCAAGGGCCACCTCCGCGGATACAATGTGACGTACTGGAGGGAGGGCAGTCAGAGGAAG
  CACAGCAAGAGACATATCCACAAAGACCATGTGGTGGTGCCCGCCAACACCACCAGTGTC
  ATCCTCAGTGGCTTGCGGCCCTATAGCTCCTACCACCTGGAGGTGCAGGCCTTTAACGGG
  CGAGGATCGGGGCCCGCCAGCGAGTTCACCTTCAGCACCCCAGAGGGAGTGCCTGGCCAC
  CCCGAGGCGTTGCACCTGGAGTGCCAGTCGAACACCAGCCTGCTGCTGCGCTGGCAGCCC
  CCACTCAGCCACAACGGCGTGCTCACCGGCTACGTGCTCTCCTACCACCCCCTGGATGAG
  GGGGGCAAGGGGCAACTGTCCTTCAACCTTCGGGACCCCGAACTTCGGACACACAACCTG
  ACCGATCTCAGCCCCCACCTGCGGTACCGCTTCCAGCTTCAGGCCACCACCAAAGAGGGC
  CCTGGTGAAGCCATCGTACGGGAAGGAGGCACTATGGCCTTGTCTGGGATCTCAGATTTT
  GGCAACATCTCAGCCACAGCGGGTGAAAACTACAGTGTCGTCTCCTGGGTCCCCAAGGAG
  GGCCAGTGCAACTTCAGGTTCCATATCTTGTTCAAAGCCTTGGGAGAAGAGAAGGGTGGG
  GCTTCCCTTTCGCCACAGTATGTCAGCTACAACCAGAGCTCCTACACGCAGTGGGACCTG
  CAGCCTGACACTGACTACGAGATCCACTTGTTTAAGGAGAGGATGTTCCGGCACCAAATG
  GCTGTGAAGACCAATGGCACAGGCCGCGTGAGGCTCCCTCCTGCTGGCTTCGCCACTGAG
  GGCTGGTTCATCGGCTTTGTGAGTGCCATCATCCTCCTGCTCCTCGTCCTGCTCATCCTC
  TGCTTCATCAAGCGCAGCAAGGGCGGCAAATACTCAGTGAAGGATAAGGAGGACACCCAG
  GTGGACTCTGAGGCCCGACCGATGAAAGATGAGACCTTCGGCGAGTACAGGTCCCTGGAG
  AGTGACAACGAGGAGAAGGCCTTTGGCAGCAGCCAGCCATCGCTCAACGGGGACATCAAG
  CCCCTGGGCAGTGACGACAGCCTGGCCGATTATGGGGGCAGCGTGGATGTTCAGTTCAAC
  GAGGATGGTTCGTTCATTGGCCAGTACAGTGGCAAGAAGGAGAAGGAGGCGGCAGGGGGC
  AATGACAGCTCAGGGGCCACTTCCCCCATCAACCCTGCCGTGGCCCTAGAATAG
  
  Clone variation with respect to NM_000425.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000425 unedited
  NTTTGTTCGAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGG
  CGGCGGGTCCGAGGCCGGCGTGCGCGGAGGCTGGGCGGGCAGCCCGAGCGGTGGCCGCAG
  CGCAGTGCCCCCACTCCCAACTCCCGCCCCAAGCCGCCCACCAGCCCCCTTCCCCTCCGG
  CCGGAGCCTGAACCGAGCCCGGCTGGCTGTGCTGCGCGGTGCCGCCGGGAAAGATGGTCG
  TGGCGCTGCGGTACGTGTGGCCTCTCCTCCTCTGCAGCCCCTGCCTGCTTATCCAGATCC
  CCGAGGAATATGAAGGACACCATGTGATGGAGCCACCTGTCATCACGGAACAGTCTCCAC
  GGCGCCTGGTTGTCTTCCCCACAGATGACATCAGCCTCAAGTGTGAGGCCAGTGGCAAGC
  CCGAAGTGCAGTTCCGCTGGACGAGGGATGGTGTCCACTTCAAACCCAAGGAAGAGCTGG
  GTGTGACCGTGTACCAGTCGCCCCACTCTGGCTCCTTCACCATCACGGGCAACAACAGCA
  ACTTTGCTCAGAGGTTCCAGGGCATCTACCGCTGCTTTGCCAGCAATAAGCTGGGCACCG
  CCATGTCCCATGAGATCCGGCTCATGGCCGAGGGTGCCCCCAAGTGGCCAAAGGAGACAG
  TGAAGCCCGTGGAGGTGGAGGAAGGGNAGTCAGTGGNTCTGCCTTGCAACCCTNCCCCCA
  GTGCAGAGCCTCTCCGGATCTACTGGATGAACAGCAAGATCTTGCACATCAAGCAGGACG
  AAGCGGGTGACGATGGCCCAGAACGGGCACCTCTACTTTGCCATGTGCTCACCTNCGACA
  CCACTCAGATACATCTGCCACGCCCACTTCCAAGCAACCAGACATCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000425 unedited
  NNTTTTACTCTGGACCGGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGAT
  TTTCAGGTTTTATTTGCCTGTTATAATTCTGATATTATGTACATATATATATATACTTTT
  GTTTTTTTGTAGAGGGCTTATCAGTAGACCAAGCACAGGCATACAGGGAGGCTGTGGCTG
  CTGAGCAGTGCTGGGAGAACCAATCTTTCCTTTTTTTTTGTCTGTTTTTATTTTTCCTGG
  GATGGGTTCATCAAAGCTAATTGCTATCAATCCTAGAAACCACCTAGGGACTCAGACATC
  TGCCTACACACTAGTGGCGTAAAGGGAAGGACAGGGGTACAAACTTTCAAAGTCACGGTG
  TATTTACATTCCCAAGGTGGGGGCGGGAGGAGGCTGAGGAAGACAACTGTTCAGACGATA
  GGGAGGGCAGGGCTGCTAAGGGGTCCTGGGGGGTGGGGGCGGCTGGAAGAGGCCGGCCAG
  TGGGCAGTGGGAGGAGGTGTGCCCGCCCCGCAGCCAGGTAGTGACGGAGGCCACTTGGAT
  GTTGTGTGGTGGGTACCGAAGGCAGCGTGTGTATGGAGCTCCTGAAAGCCGGCATGGGGT
  GGGCTGGGCGGCGGGAGAAGGCGGTGCTGCCAGAGTGCGATGCTGGGGAGTGGNGGGACT
  CTGGGCTCTNCAGATGGCCTCCCCTGCCCCCGCGGCCAGTTTGCCCGGCTCCCATCCCGG
  TGGAGCANAGATGGCAAAGAAAAGAAGCAGATGGTGGGGACACCCGGTTCTGTTCCCAGT
  CCCACCCAAGCTGCTGGGGAATGGCTTGGGGAGAGGGAAAAAGGGCCAACCTTGGTCCTG
  AGGAAACCCCCCATTTTTGTCCCCACTTCCGGCACCAAGAAAGACAAATTTTCGGGAAAC
  ACTTTTGGGCCACCCCTTACCTTTTCTTCCCCAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000425
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: A TrueClone.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000425.2, NP_000416.1
• RefSeq Size: 4525 bp
• RefSeq ORF: 3774 bp
• Locus ID: 3897
• UniProt ID: P32004
• Cytogenetics: Xq28
• Domains: ig, IGc2, IG, FN3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Axon guidance, Cell adhesion molecules (CAMs)
• Gene Summary: The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (1, also known as FL-L1CAM) differs in the 5' UTR and represents use of an alternate promoter, compared to variant 4. Variants 1 and 4 encode the same isoform (1).