KRAS (NM_004985) Human Untagged Clone

CAT#: SC109374

KRAS (untagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRAS
• Synonyms: C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004985 edited
  GAATTCGGCACGAGGCGGCGGAGGCAGCAGCGGCGGCGGCAGTGGCGGCGGCGAAGGTGG
  CGGCGGCTCGGCCAGTACTCCCGGCCCCCGCCATTTCGGACTGGGAGCGAGCGCGGCGCA
  GGCACTGAAGGCGGCGGCGGGGCCAGAGGCTCAGCGGCTCCCAGGTGCGGGAGAGAGGCC
  TGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTG
  CCTTGACGATACAGCTAATTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAGG
  ATTCCTACAGGAAGCAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTCGACA
  CAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGGACTGGGGAGGGCT
  TTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGAG
  AACAAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAAAT
  GTGATTTGCCTTCTAGAACAGTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATG
  GAATTCCTTTTATTGAAACATCAGCAAAGACAAGACAGGGTGTTGATGATGCCTTCTATA
  CATTAGTTCGAGAAATTCGAAAACATAAAGAAAAGATGAGCAAAGATGGTAAAAAGAAGA
  AAAAGAAGTCAAAGACAAAGTGTGTAATTATGTAAATACAATTTGTACTTTTTTCTTAAG
  GCATACTAGTACAAGTGGTAATTTTTGTACATTACACTAAATTATTAGCATTTGTTTTAG
  CATTACCTAATTTTTTTCCTGCTCCATGCAGACTGTTAGCTTTTACCTTAAATGCTTATT
  TTAAAATGACAGTGGAAGTTTTTTTTTCCTCGAAGTGCCAGTATTCCCAGAGTTTTGGTT
  TTTGAACTAGCAATGCCTGTGAAAAAGAAACTGAATACCTAAGATTTCTGTCTTGGGGTT
  TTTGGTGCATGCAGTTGATTACTTCTTATTTTTCTTACCAATTGTGAATGTTGGTGTGAA
  ACAAATTAATGAAGCTTTTGAATCATCCCTATTCTGTGTTTTATCTAGTCACATAAATGG
  ATTAATTACTAATTTCAGTTGAGACCTTCTAATTGGTTTTTACTGAAACATTGAGGGAAC
  ACAAATTTATGGGCTTCCTGATGATGATTCTTCTAGGCATCATGTCCTATAGTTTGTCAT
  CCCTGACGAATGTAAAGXXXXXXXXXXXXXXXXXXXXXXXXAATGGTTTCTTGATAATTG
  TGTAGTAATGTTTTTTAGAACCCAGCAGTTACCTTAAAGCTGAATTTATATTTAGTAACT
  TCTGTGTTAATACTGGATAGCATGAATTCTGCATTGAGAACCTGAATAGCTGTCATAAAA
  TGAAACTTTCTTTCTAAAGAAAGATACTCACATGAGTTCTTGAAGAATAGTCATAACTAG
  ATTAAGATCTGTGTTTTAGTTTAATAGTTTGAAGTGCCTGTTTGGGATAATGATAGGTAA
  TTTAGATGAATTTAGGGGAAAAAAGTTATCTGCAGAAATGTTGAGGGCCCATCTCTCCCC
  CCACACCCCCACAGAGCTAACTGGGTTACAGTGTTTTATCCGAAAGTTTCCAATTCCACT
  GTCTTGTGTTTTCATGTTGAAAATACTTTTGCATTTTTCCTTTGAGTGCCAATTTCTTAC
  TAGTACTATTTCTTAATGTAACATGTTTACCTGGAATGTATTTTAACTATTTTTGTATAG
  TGTAAACTGAAACATGCACATTTTGTACATTGTGCTTTCTTTTGTGGGACATATGCAGTG
  TGATCCAGTTGTTTTCCATCATTTGGTTGCGCTGACCTAGGAATGTTGGTCATATCAAAC
  ATTAAAAATGACCACTCTTTTAATTGAAATTAACTTTTAAATGTTTATAGGAGTATGTGC
  TGTGAAGTGATCTAAAATTTGTAATATTTTTGTCATGAACTGTACTACTCCTAATTATTG
  TAATGTAATAAAAATAGTTACAGTGACAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004985 unedited
  TATTACACCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGCGGCGGAGGCAGCAGCGGCGGCGGCAGTGGCGGCGGCGAAGGTG
  GCGGCGGCTCGGCCAGTACTCCCGGCCCCCGCCATTTCGGACTGGGAGCGAGCGCGGCGC
  AGGCACTGAAGGCGGCGGCGGGGCCAGAGGCTCAGCGGCTCCCAGGTGCGGGAGAGAGGC
  CTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGT
  GCCTTGACGATACAGCTAATTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAG
  GATTCCTACAGGAAGCAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTCGAC
  ACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGGACTGGGGAGGGC
  TTTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGA
  GAACAAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAAA
  TGTGATTTGCCTTCTAGAACAGTAGACACANAACAGGCTCAGGACTTAGCAAGAAGTTAT
  GGAATTCCTTNTATTGAAACATCAGCAAAGACAAGACAGGGTGTTGATGATGCCTTCTAT
  ACATTAGNTCGAGAAATTNCGAAACATANAGAANAGATGAGCANAGATGGGTAAAAGAAG
  AAAAAGAAGTCNAAGACAAGTGTGTAANTATGTAAATACCATTNGTACTTTTNTCTTAAG
  CATACTAGTACAAGGGGTAATTTTTGTACATTACACTAAATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004985 unedited
  NGGGAAATCTATGNACCGCGCCGCATTCTANGTCGAGTTTTTTTTTTTTTTTTTTGTCAC
  TGTAACTATTTTTATTACATTACAATAATTAGGAGTAGTACAGTTCATGACAAAAATTTA
  CAAATTTTAGATCACTTCACAGCACATACTCCTATAAACATTTAAAAGTTAATTTCAATT
  AAAAGAGTGGTCATTTTTAATGTTTGATATGACCAACATTCCTAGGTCAGCGCAACCAAA
  TGAATGAAAACAACTGGATCACACTGCATATGTCCCACAAAAGAAAGCACAATGTACAAA
  ATGTGCATGTTTCAGTTTACACTATACAAAAATAGTTAAAATACATTCCAGGTAAACATG
  TTACATTAAGAAATAGTACTAGTAAGAAATTGGCACTCAAAGGAAAAATGCAAAAGTATT
  TTCAACATGAAAACACAAGACAGTGGAATTGAAAACTTTCGGATAAAACACTGTAACCCA
  GTTAGCTCTGTGGGGGTGTGGGGGGAAGAGATGGGCCCTCAACATTTCTGCAGATAACTT
  TTTTCCCCTAAATTCATCTAAATTACCTATCATTATCCCAAACAGGCACTTCAAACTATT
  AAACTAAAACACAGATCTTAATCTAGTTATGACTATTCTTCAAGAACTCATGTGAGTATC
  TTTCTTTAGAAAGAAAGTTTCATTTTATGACAGCTATTCACGTTCTCAATGCAGAATTCA
  TGCTATCCAGTATTAACACAGAAAGTACTAAATATAAATTCAGCTTTAAGGTAACTGCTG
  GGTNTCTAAAAACATTTCTACACAATTATCAAGAAACATTACTTTTTGACAATGGGAATC
  TTAAATAGTTTTGCTGTCTAAAAAAAATCCCTAAAAAGTTATTACTGGTTGAAGAAAAAT
  GTTAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004985
• Insert Size: 2067 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004985.3, NP_004976.2
• RefSeq Size: 5312 bp
• RefSeq ORF: 567 bp
• Locus ID: 3845
• UniProt ID: P01116
• Cytogenetics: 12p12.1
• Domains: ras, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway
• Gene Summary: This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (b) is composed of five exons and lacks exon 4a which the longer transcript variant (a) includes. This predominant variant (b) has a cds that terminates in exon 4b and encodes isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.