Factor XI (F11) (NM_000128) Human Untagged Clone

CAT#: SC109207

F11 (untagged)-Human coagulation factor XI (F11)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Factor XI
• Synonyms: FXI; PTA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109207 sequence for NM_000128 edited (data generated by NextGen Sequencing)
  ATGATTTTCTTATATCAAGTGGTACATTTCATTTTATTTACTTCAGTTTCTGGTGAATGT
  GTGACTCAGTTGTTGAAGGACACCTGCTTTGAAGGAGGGGACATTACTACGGTCTTCACA
  CCAAGCGCCAAGTACTGCCAGGTAGTCTGCACTTACCACCCAAGATGTTTACTCTTCACT
  TTCACGGCGGAATCACCATCTGAGGATCCCACCCGATGGTTTACTTGTGTCCTGAAAGAC
  AGTGTTACAGAAACACTGCCAAGAGTGAATAGGACAGCAGCGATTTCTGGGTATTCTTTC
  AAGCAATGCTCACACCAAATAAGCGCTTGCAACAAAGACATTTATGTGGACCTAGACATG
  AAGGGCATAAACTATAACAGCTCAGTTGCCAAGAGTGCTCAAGAATGCCAAGAAAGATGC
  ACGGATGACGTCCACTGCCACTTTTTCACGTACGCCACAAGGCAGTTTCCCAGCCTGGAG
  CATCGTAACATTTGTCTACTGAAGCACACCCAAACAGGGACACCAACCAGAATAACGAAG
  CTCGATAAAGTGGTGTCTGGATTTTCACTGAAATCCTGTGCACTTTCTAATCTGGCTTGT
  ATTAGGGACATTTTCCCTAATACGGTGTTTGCAGACAGCAACATCGACAGTGTCATGGCT
  CCCGATGCTTTTGTCTGTGGCCGAATCTGCACTCATCATCCCGGTTGCTTGTTTTTTACC
  TTCTTTTCCCAGGAATGGCCCAAAGAATCTCAAAGAAATCTTTGTCTCCTTAAAACATCT
  GAGAGTGGATTGCCCAGTACACGCATTAAAAAGAGCAAAGCTCTTTCTGGTTTCAGTCTA
  CAAAGCTGCAGGCACAGCATCCCAGTGTTCTGCCATTCTTCATTTTACCATGACACTGAT
  TTCTTGGGAGAAGAACTGGATATTGTTGCTGCAAAAAGTCACGAGGCCTGCCAGAAACTG
  TGCACCAATGCCGTCCGCTGCCAGTTTTTTACCTATACCCCAGCCCAAGCATCCTGCAAC
  GAAGGGAAGGGCAAGTGTTACTTAAAGCTTTCTTCAAACGGATCTCCAACTAAAATACTT
  CACGGGAGAGGAGGCATCTCTGGATACACATTAAGGTTGTGTAAAATGGATAATGAGTGT
  ACCACCAAAATCAAGCCCAGGATCGTTGGAGGAACTGCGTCTGTTCGTGGTGAGTGGCCG
  TGGCAGGTGACCCTGCACACAACCTCACCCACTCAGAGACACCTGTGTGGAGGCTCCATC
  ATTGGAAACCAGTGGATATTAACAGCCGCTCACTGTTTCTATGGGGTAGAGTCACCTAAG
  ATTTTGCGTGTCTACAGTGGCATTTTAAATCAATCTGAAATAAAAGAGGACACATCTTTC
  TTTGGGGTTCAAGAAATAATAATCCATGATCAGTATAAAATGGCAGAAAGCGGGTATGAT
  ATTGCCTTGTTGAAACTGGAAACCACAGTGAATTACACAGATTCTCAACGACCCATATGC
  CTGCCTTCCAAAGGAGATAGAAATGTAATATACACTGATTGCTGGGTGACTGGATGGGGG
  TACAGAAAACTAAGAGACAAAATACAAAATACTCTCCAGAAAGCCAAGATACCCTTAGTG
  ACCAACGAAGAGTGCCAGAAGAGATACAGAGGACATAAAATAACCCATAAGATGATCTGT
  GCCGGCTACAGGGAAGGAGGGAAGGACGCTTGCAAGGGAGATTCGGGAGGCCCTCTGTCC
  TGCAAACACAATGAGGTCTGGCATCTGGTAGGCATCACGAGCTGGGGCGAAGGCTGTGCT
  CAAAGGGAGCGGCCAGGTGTTTACACCAACGTGGTCGAGTACGTGGACTGGATTCTGGAG
  AAAACTCAAGCAGTGTGA
  
  Clone variation with respect to NM_000128.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000128 unedited
  NGGGCTGTCAGAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGCTCATT
  CAGAGGAGGATGAAGACCTTTTGGAGGAAGAAAACACCCTTATTAAGAATTGCAGCAAGT
  AAGCCAACAAGGTCTTTTCAGGATGATTTTCTTATATCAAGTGGTACATTTCATTTTATT
  TACTTCAGTTTCTGGTGAATGTGTGACTCAGTTGTTGAAGGACACCTGCTTTGAAGGAGG
  GGACATTACTACGGTCTTCACACCAAGCGCCAAGTACTGCCAGGTAGTCTGCACTTACCA
  CCCAAGATGTTTACTCTTCACTTTCACGGCGGAATCACCATCTGAGGATCCCACCCGATG
  GTTTACTTGTGTCCTGAAAGACAGTGTTACAGAAACACTGCCAAGAGTGAATAGGACAGC
  AGCGATTTCTGGGTATTCTTTCAAGCAATGCTCACACCAAATAAGCGCTTGCAACAAAGA
  CATTTATGTGGACCTAGACATGAAGGGCATAAACTATAACAGCTCAGTTGCCAAGAGTGC
  TCAAGAATGCCAAGAAAGATGCACGGATGACGTCCACTGCCACTTTTTCACGTACGCCAC
  AAGGCAGTTTCCCAGCCTGGAGCATCGTAACATTTGTCTACTGAAGCACACCCAAACAGG
  GACACCAACCAGAATAACGAAGCTCNGATAAAGTGGTGTCTGGGATTTTCACTGANATCC
  TGTGCACTNTCTAATCTGGCTTGTATTAAGGACATTNTCCCCTATACNGTGTTTGCAGAC
  AGCACATCGACAGTGTCATGGCTNCCCGATGCTTTGTCTGTGGCCGAATCTGCACTATCA
  TNCCGGNTGCTNGNTTTTTACCTTNNCTTTCCCAGNAATGGCCCAAGATCTCAAGAATAC
  TTNGTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000128
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000128.2, NP_000119.1
• RefSeq Size: 2217 bp
• RefSeq ORF: 1878 bp
• Locus ID: 2160
• UniProt ID: P03951
• Cytogenetics: 4q35.2
• Domains: APPLE, Tryp_SPc, PAN
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).