Factor VIII (F8) (NM_019863) Human Untagged Clone

CAT#: SC109206

F8 (untagged)-Human coagulation factor VIII, procoagulant component (F8), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Factor VIII
• Synonyms: AHF; DXS1253E; F8B; F8C; FVIII; HEMA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109206 sequence for NM_019863 edited (data generated by NextGen Sequencing)
  ATGCGGATCCAAGACCCTGGGAAGGTCTTCTTTGGCAATGTGGATTCATCTGGGATAAAA
  CACAATATTTTTAACCCTCCAATTATTGCTCGATACATCCGTTTGCACCCAACTCATTAT
  AGCATTCGCAGCACTCTTCGCATGGAGTTGATGGGCTGTGATTTAAATAGTTGCAGCATG
  CCATTGGGAATGGAGAGTAAAGCAATATCAGATGCACAGATTACTGCTTCATCCTACTTT
  ACCAATATGTTTGCCACCTGGTCTCCTTCAAAAGCTCGACTTCACCTCCAAGGGAGGAGT
  AATGCCTGGAGACCTCAGGTGAATAATCCAAAAGAGTGGCTGCAAGTGGACTTCCAGAAG
  ACAATGAAAGTCACAGGAGTAACTACTCAGGGAGTAAAATCTCTGCTTACCAGCATGTAT
  GTGAAGGAGTTCCTCATCTCCAGCAGTCAAGATGGCCATCAGTGGACTCTCTTTTTTCAG
  AATGGCAAAGTAAAGGTTTTTCAGGGAAATCAAGACTCCTTCACACCTGTGGTGAACTCT
  CTAGACCCACCGTTACTGACTCGCTACCTTCGAATTCACCCCCAGAGTTGGGTGCACCAG
  ATTGCCCTGAGGATGGAGGTTCTGGGCTGCGAGGCACAGGACCTCTACTGA
  
  Clone variation with respect to NM_019863.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019863 unedited
  GGTCGCATTTCGACACACGTTTCACTATAGGCGGCCGCGCAATTCCGCACGAGGCCCTGA
  TGACGTGCAAAGAGCGGCATCGGAGGCGGGGCCTGGCCGGGCTGTGAGCGGCGTATGCAA
  ATCGAGGGTCTCGGGGATGCGGATCCAAGACCCTGGGAAGGTCTTCTTTGGCAATGTGGA
  TTCATCTGGGATAAAACACAATATTTTTAACTCTTTTTATTATTGCTCGATACATCCGTT
  TGGACCCAACTCATTATAGCATTCGCAGCACTCTTCGCATGGAGTTGATGGGCTGTGATT
  CGAATAGTTGCAGCATGCCATTGGGAATGGAGAGTAAAGCAATATCAGATGCACAGATTA
  CTGCTTCATCCTACTTTACCAATATGTTTGCCACCTGGTCTCCTTCAAAAGCTCGACTTC
  ACCTCCAAGGGAGGAGTAATGCCTGGAGACCTCAGGCGAATAATCCAAAAGAGTGGCTGC
  AAGTGGACTTCCAGAAGACAATGAAAGTCACAGGAGTAACTACTCACGGAGTAAAATCTC
  TGCTTACCAGCATGTATGTGAAGGAGTTTCTCATCTCCACCAGCCAAGACGGTCATCAGC
  GCACTCTCTCTTTCCACAATGGGACAGTAAAGGTTCCTTAGGGAAATCAAGACTCCTTCA
  CACCTGTGGTGAACTCTCTAGACCCACCGTTACTGACTCGCTACCTTCGAATTCACCCCC
  ACACTTGGGTGCACCCCATTGCCCTGAGGACGGAGGTTCTGGGCTTGCAAGCACAGGACC
  TTCTACTGAGGCGGCCACTGCAGCACCTGCAACTGGCGCAACTCTCCCTCCTCAGCCTCA
  GGGGAGTGTCCCTCCCTGGCTTGCGTCTTACCTTCGCGCCAAACCTACCAAAACTCGCTC
  TGAACCCTCCGGAATCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019863 unedited
  ACTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTATGTGTC
  AATGTTTTATTTAACTCATTACTGAAGAAACCAGCAGGAAAATAAAAGAGCTAGTTCCAT
  GAACATTTGAGAAATAGAGATGTATATAGTCAATGGGAAAAGAATGCCAAAATAAGATTA
  TTAAGTTAAACATAACAGGAAATAAAACCATATCACCTTTGGGGTTAACTCCTCTACCGG
  GCTATAAAACAAAACAACTTATTCATTTCTGTAAGTTAGCCTCTAAACTTTAAGAACTTC
  ACAGGGCTATAAACCATTTGCACCTGCATCATTTGTGGATTGTGACTATACTGTGACAGC
  TATGTTATAGTTTCCAAAGAGGTCAGATAAGGGTCAAGCAGGATTATTAGAAAATACTCC
  AGCATAGAAAAAAGTAGCCATTCTTTTGCCTTTTCTCAGATTGGGATCCATTTTTCTTTC
  ATATTAGGATCTCCTGTTTTCCATTTGCCCTGGGTTGCAATCTTTGGATGCTTCCTCCAA
  CTGCTCTATAACTTATCCTTGCCTCCAGCCCCCTTTACTATCAGGAGATTCTGTGTAGTC
  ATAGTGAAGGGGTCAGGCAGATGGAAGGAGCAGTAAACCCTTCACAATCTTATGGGGGTG
  GAGGGCAAGAANGGGGATCCTATTGTGTGGTGATATGGCAGACTGGAGTGTTTTTTCTGT
  TTTCACCAGTCCAATTTTCTTCTGACCTCCTTGATATTTCAACTATGTAAGTAGGACTCT
  AGGTTTCAGTCACGGTATATAATTTCCCCTGTTACATTGTTTGTTGGGTTATTTTCAGCA
  GGAAATAGTAGAGGGAGAAAGTAAACTGATTGCATTATTAGATTACAAATTTGTATCTCC
  TTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_019863
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019863.2, NP_063916.1
• RefSeq Size: 2617 bp
• RefSeq ORF: 651 bp
• Locus ID: 2157
• UniProt ID: P00451
• Cytogenetics: Xq28
• Domains: F5_F8_type_C
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) contains an unique 5' exon located within intron 22 of transcript variant 1. This exon codes for eight amino acids and is spliced to exons 23-26 maintaining the reading frame. The resulting isoform (b) is considerably shorter compared to isoform a, and includes the phospholipid binding domain.