Factor VII (F7) (NM_019616) Human Untagged Clone

CAT#: SC109205

F7 (untagged)-Human coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Factor VII
• Synonyms: SPCA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109205 sequence for NM_019616 edited (data generated by NextGen Sequencing)
  ATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGGGCTTCAGGGCTGCCTGGCT
  GCAGTCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCACCGGCGCCGGCGCGCCAAC
  GCGTTCCTGGAGGAGCTACGGCCGGGCTCCCTGGAGAGGGAGTGCAAGGAGGAGCAGTGC
  TCCTTCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAGGACGAAGCTGTTCTGGATT
  TCTTACAGTGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAG
  GACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAG
  ACGCACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGGCGGCTGTGAGCAGTACTGC
  AGTGACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGAGGGGTACTCTCTGCTGGCA
  GACGGGGTGTCCTGCACACCCACAGTTGAATATCCATGTGGAAAAATACCTATTCTAGAA
  AAAAGAAATGCCAGCAAACCCCAAGGCCGAATTGTGGGGGGCAAGGTGTGCCCCAAAGGG
  GAGTGTCCATGGCAGGTCCTGTTGTTGGTGAATGGAGCTCAGTTGTGTGGGGGGACCCTG
  ATCAACACCATCTGGGTGGTCTCCGCGGCCCACTGTTTCGACAAAATCAAGAACTGGAGG
  AACCTGATCGCGGTGCTGGGCGAGCACGACCTCAGCGAGCACGACGGGGATGAGCAGAGC
  CGGCGGGTGGCGCAGGTCATCATCCCCAGCACGTACGTCCCGGGCACCACCAACCACGAC
  ATCGCGCTGCTCCGCCTGCACCAGCCCGTGGTCCTCACTGACCATGTGGTGCCCCTCTGC
  CTGCCCGAACGGACGTTCTCTGAGAGGACGCTGGCCTTCGTGCGCTTCTCATTGGTCAGC
  GGCTGGGGCCAGCTGCTGGACCGTGGCGCCACGGCCCTGGAGCTCATGGTCCTCAACGTG
  CCCCGGCTGATGACCCAGGACTGCCTGCAGCAGTCACGGAAGGTGGGAGACTCCCCAAAT
  ATCACGGAGTACATGTTCTGTGCCGGCTACTCGGATGGCAGCAAGGACTCCTGCAAGGGG
  GACAGTGGAGGCCCACATGCCACCCACTACCGGGGCACGTGGTACCTGACGGGCATCGTC
  AGCTGGGGCCAGGGCTGCGCAACCGTGGGCCACTTTGGGGTGTACACCAGGGTCTCCCAG
  TACATCGAGTGGCTGCAAAAGCTCATGCGCTCAGAGCCACGCCCAGGAGTCCTCCTGCGA
  GCCCCATTTCCCTAG
  
  Clone variation with respect to NM_019616.2
  138 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019616 unedited
  GGGCATTATTCGGATACGACTCATATAGGCGGCCGTCGAATTCGCACGAGGGCAGGGGCA
  GCACTGCAGAGATTTCATCATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGG
  GCTTCAGGGCTGCCTGGCTGCAGTCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCA
  CCGGCGCCGGCGCGCCAACGCGTTCCTGGAGGAGCTACGGCCGGGCTCCCTGGAGAGGGA
  GTGCAAGGAGGAGCAGTGCTCCTTCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAG
  GACGAAGCTGTTCTGGATTTCTTACAGTGATGGGGACCAGTGTGCCTCAAGTCCATGCCA
  GAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTT
  CGAGGGCCGGAACTGTGAGACGCACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGG
  CGGCTGTGAGCAGTACTGCAGTGACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGA
  GGGGTACTCTCTGCTGGCAGACGGNGTGTCCTGCACACCCACAGTTGAATATCCATGTGG
  AAAAATACCTATTCTAGAAAAAAGAAATGCCAGNCAACCCCAAGGCCGAATTGTGGGGGG
  CAAGGTGTGCCCCANAGGGGAGTGTCCATGGCANGTCCTGTTGTTGGTGAATGGAGCTCA
  TTTGTGTGGGGGGGACCCTGATCAACACCATCTGGGGTGGTCTCCGCGGCCCACTGGTTT
  CGACAAAATCAAGAACTTGGAGAACCTGATCGCCGTGCTGGGCGAGCACGACCTCAGCGA
  GCACGACGGGGATGACCAAAGCCCGCGGGTGGCGCCAGTAATTATCCCCACACGTACGTC
  CCGGGCACCACCAACCATAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019616 unedited
  NGCCCCTACTTGAACCCGGCCGCTTTTTAGATCGAGTTTTTTTTTTTTTTTTTTTTTTGG
  CAGGCTCCACAGGTGTGCGGAGGAGACGCAGCCGTTTATTGTGAAGCTGGGAAGCAGGTG
  GGGAATATCTGTGCACCCGCCCCACGAAGGAGAAATACATTTGGGGGAGAGTTTGGGGGC
  ATTCGCCACCATGCATGGGGATGCTTCTGAATTGTCTGAAGTGAAGATGATAATGGATAA
  GAACCAGAAAAGTGAGAGCTAAACAACCGCCTTCAGAGGACAGCACACATGGAGTCAGCA
  TCGGTGTGTGTGCATCTGTGTGTGCATCTGTGTGTGTACATCGGTGTGTGTGCGCATTGG
  TGTGTGTGTGCTCATCCATGTGTGTGCATATCTGTGTGTGCGCACATCGGTGTGTGCATA
  TCTCTGTGCATCCGTGTGCACTGATGTGTGCGTGCATTGGCATGTGCGTGCATCCATGTG
  TATATCTGTGTGTGCATCTGTGTGTGCATATCTCTATGTGCGTGTGCATCGGTGTGTTTG
  CGTATCTCTGTGTGACCATCTGTGTGTGCATCCGTGTGTGTGCATATCTCTGTGTGTGTG
  CATTGGCGTGTGTGTGTGTGCATCCATGTGTGTTCATGCCTCTACTGGCTCCAGCAATGA
  AGGCAGAGCATCAGCCTTGAGTCCACCACATCACTGCCCAGTTGCCTTGTCTGAAAGGAG
  GTAAAAGAAGCCTCACCTGGGCTTGTCTGCCTCCAGAACAACCTTGGGCACCTCCCCTGC
  CCCCTTTGTACAACGGCCTGTTGGCCTTTGCCTTTCATTTTTTTTATACCCTCCCTGGGG
  CCCTTTATACCCTCTTGAATCTTGGAAGCTTTTTGGAGTCCCTCCCCCTGGTCCCAAAAT
  TTCTCCCTTAAGTTTTCTTTGTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_019616
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019616.1, NP_062562.1
• RefSeq Size: 2412 bp
• RefSeq ORF: 1335 bp
• Locus ID: 2155
• UniProt ID: P08709
• Cytogenetics: 13q34
• Domains: GLA, Tryp_SPc, EGF_CA, EGF, EGF
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a. This isoform (b) may undergo proteolytic processing similar to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. A downstream translational start codon is selected for this RefSeq based on its better conservation in mammalian species. An upstream in-frame start codon is also present and would result in a protein that is 15 aa longer at the N-terminus, but this start codon is poorly conserved and located within a protein-binding site identified in the promoter region, as described in PubMed: 8576177. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon.