Cathepsin B (CTSB) (NM_147781) Human Untagged Clone

CAT#: SC109130

CTSB (untagged)-Human cathepsin B (CTSB), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cathepsin B
• Synonyms: APPS; CPSB; RECEUP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_147781, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGCAGCTCTGGGCCTCCCTCTGCTGCCTGCTGGTGTTGGCCAATGCCCGGAGCAGGCCCTCTTTCC
  ATCCCCTGTCGGATGAGCTGGTCAACTATGTCAACAAACGGAATACCACGTGGCAGGCCGGGCACAACTT
  CTACAACGTGGACATGAGCTACTTGAAGAGGCTATGTGGTACCTTCCTGGGTGGGCCCAAGCCACCCCAG
  AGAGTTATGTTTACCGAGGACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTC
  CCACCATCAAAGAGATCAGAGACCAGGGCTCCTGTGGCTCCTGCTGGGCCTTCGGGGCTGTGGAAGCCAT
  CTCTGACCGGATCTGCATCCACACCAATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGGACCTGCTCACA
  TGCTGTGGCAGCATGTGTGGGGACGGCTGTAATGGTGGCTATCCTGCTGAAGCTTGGAACTTCTGGACAA
  GAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCATGTAGGGTGCAGACCGTACTCCATCCCTCCCTG
  TGAGCACCACGTCAACGGCTCCCGGCCCCCATGCACGGGGGAGGGAGATACCCCCAAGTGTAGCAAGATC
  TGTGAGCCTGGCTACAGCCCGACCTACAAACAGGACAAGCACTACGGATACAATTCCTACAGCGTCTCCA
  ATAGCGAGAAGGACATCATGGCCGAGATCTACAAAAACGGCCCCGTGGAGGGAGCTTTCTCTGTGTATTC
  GGACTTCCTGCTCTACAAGTCAGGAGTGTACCAACACGTCACCGGAGAGATGATGGGTGGCCATGCCATC
  CGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCCTACTGGCTGGTTGCCAACTCCTGGAACACTGACT
  GGGGTGACAATGGCTTCTTTAAAATACTCAGAGGACAGGATCACTGTGGAATCGAATCAGAAGTGGTGGC
  TGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_147781 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTGGGTGGATCTAGGATC
  CGGCTTCCAACATGTGGCAGCTCTGGGCCTCCCTCTGCTGCCTGCTGGTGTTGGCCAATG
  CCCGGAGCAGGCCCTCTTTCCATCCCCTGTCGGATGAGCTGGTCAACTATGTCAACAAAC
  GGAATACCACGTGGCAGGCCGGGCACAACTTCTACAACGTGGACATGAGCTACTTGAAGA
  GGCTATGTGGTACCTTCCTGGGTGGGCCCAAGCCACCCCAGAGAGTTATGTTTACCGAGG
  ACCTGAAGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCA
  AAGAGATCAGAGACCAGGGCTCCTGTGGCTCCTGCTGGGCCTTCGGGGCTGTGGAAGCCA
  TCTCTGACCGGATCTGCACGCACACCAATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGG
  ACCTGCTCACATGCTGTGGCAGCATGTGTGGGGACGGCTGTAATGGTGGCTATCCTGCTG
  AAGCTTGGAACTTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCATG
  TAGGGTGCAGACCGTACTCCATCCCTCCCTGTGAGCACCACGTCAACGGCTCCCGGCCCC
  CATGCACGGNGGAGGGGAGATACCCNCAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCC
  CGACCTACANACAGGGACAGCACTACGGATACAATTCCTACAGCGTCTCCAATAGCGAGA
  AGGACATCATGGCCCGAGATCTCCCCAAACGGNCCCGTGGNAGGAGCTTTCTCTGTGTAT
  TCGGACTTCCTGCTCTACAGTCAGGAGTGTACAACACGTCACGGAGAGATGATGGTGGCC
  ATGCATNCGCATNCTGGGCTGGGAGTGGAGATGGCACACCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_147781 unedited
  GTCATGGACCGCGCCGCAATCTANGATCGAGTTATTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTAGTTGGGAAAAACCTTTTATTGGCACAGGCATTCCTTGTTA
  ACTTGACAGGGTGAAGCTGTAATTTTTCAAAAACAGTAAAGGCTGGTTTCTCCTAAACTA
  TTTTCCTTGGGGTAGTAAAAATACAGTGGGTCAAAAACAACTCCTGACCACTTGGTTTCC
  TTTTGAGCCGCGTCATTAGGAGGCAATCTGTAAAACTAGCACAGGTCTCCCGCTGTTCCA
  CTGGCTCACCCACATGATTAGCAGAGTGCACCAAAAAATAAAACTTCTATTAAAGAATCA
  TGCTGAGCACAACATCAGAGAGGTTGTGACATTGCAAACTCGATAAATGCAGGGGGCCTG
  GGAAACTGGCGTTCTCCAAAGGGCTCCCAACACCATCTCTCCTTTGATTTCTGTGACAAA
  TGTGGAAAGCTACTTGCTTGGAGGTACTGGGGGAACTGATGGGGGAACTTTCATCCTGTT
  AGGAACTCCGCTTTCCATTCCTGCGTCTCTGTCTTGCTCCCTGGAGATGGATGGATCACG
  GAGGGGGCCACAGTCCAGCTGGGGCAGCAAGTACTCCCTACGGCACTTGTCTACAGGGGG
  AAAGACCCTCTGTGCTTGGCCCGGTGGGCTCACATGGCCTGTTTGCCCTGTAACCCCCGG
  CTGGGATGGAACCCCGACTTGGTCTCCTTTGAAAAACAGTCTGAAGTTTGGGCCAATCCG
  TCCCTTTAACCCTGCCTGGAACTTTTTTCTTTACGGGAATTAAAAGAATAAAAAGCGCTT
  TTCTCCCCGTATTCCCCCCCCAGAACTTGCACCCAAGGGCCCCAGGGAGATATAATTTTT
  TTCCCATTCCTGTTCGCGGGCGTGGAAATTCCGACCCCCCTTTTTTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_147781
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_147781.2, NP_680091.1
• RefSeq Size: 3902 bp
• RefSeq ORF: 1020 bp
• Locus ID: 1508
• UniProt ID: P07858
• Cytogenetics: 8p23.1
• Domains: Pept_C1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Antigen processing and presentation, Lysosome
• Gene Summary: This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors. Both Cathepsin B and Cathepsin L are involved in the cleavage of the spike protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) upon its entry to the human host cell. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Sep 2020]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1-5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.