p38 (CRK) (NM_005206) Human Untagged Clone

CAT#: SC109105

CRK (untagged)-Human v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant I

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: p38
• Synonyms: CRKII; p38
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005206, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGGCAACTTCGACTCGGAGGAGCGGAGTAGCTGGTACTGGGGGAGGTTGAGTCGGCAGGAGGCGG
  TGGCGCTGCTGCAGGGCCAGCGGCACGGGGTGTTCCTGGTGCGGGACTCGAGCACCAGCCCCGGGGACTA
  TGTGCTCAGCGTCTCAGAGAACTCGCGCGTCTCCCACTACATCATCAACAGCAGCGGCCCGCGCCCGCCG
  GTGCCACCGTCGCCCGCCCAGCCTCCGCCCGGGGTGAGCCCCTCCAGACTCCGAATAGGAGATCAAGAGT
  TTGATTCATTGCCTGCTTTACTGGAATTCTACAAAATACACTATTTGGACACTACAACGTTGATAGAACC
  AGTTTCCAGATCCAGGCAGGGTAGTGGAGTGATTCTCAGGCAGGAGGAGGCGGAGTATGTGCGAGCCCTC
  TTTGACTTTAATGGGAATGATGAGGAAGATCTTCCCTTTAAGAAAGGAGACATCTTGAGAATCCGGGACA
  AGCCTGAAGAGCAGTGGTGGAATGCGGAGGACAGCGAAGGCAAGAGAGGGATGATTCCAGTCCCTTACGT
  CGAGAAGTATAGACCTGCCTCCGCCTCAGTATCGGCTCTGATTGGAGGTCGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005206 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTCGGGCGCTGCTG
  TGAAGCTGAAACCGGAGCCGGTCCGCTGGGCGGCGGGCGCCGGGGGCCGGAGGGGCGCGC
  GCGGCGGCGGCACCCCAGCGTTTAGGCGCGGAGGCAGCCATGGCGGGCAACTTCGACTCG
  GAGGAGCGGAGTAGCTGGTACTGGGGGCGGTTGAGTCGGCAGGAGGCGGTGGCGCTGCTG
  CAGGGCCAGCGGCACGGGGTGTTCCTGGTGCGGGACTCGAGCACCAGCCCCGGGGACTAT
  GTGCTCAGCGTCTCAGAGAACTCGCGCGTCTCCCACTACATCATCAACAGCAGCGGCCCG
  CGCCCGCCGGTGCCACCGTCGCCCGCCCAGCCTCCGCCCGGGGTGAGCCCCTCCAGACTC
  CGAATAGGAGATCAAGAGTTTGATTCATTGCCTGCTTTACTGGAATTCTACAAAATACAC
  TATTTGGACACTACAACGTTGATAGAACCAGTTTCCAGATCCAGGCAGGGTAGTGGAGTG
  ATTCTCAGGCAGGAGGAGGCGGAGTATGTGCGAGCCCTCTTTGACTTTAATGGGAATGAT
  GAGGAAGATCTTCCCTTTAAGAAAGGAGACATCTTGAGAATCCGGGACAAGCCTGAAGAG
  CAGTGGTGGAATGCGGAGGACAGCGNAAGCAAGAGAGGGATGATTCCAGTCCCTTACGTC
  GAGAAGTATAGACCTGCCTNCGCCTCAGTATCGGCTCTGATTGGAGGGTCGTGAGCTGGT
  AAAGGNTACGAAGATTAATGTGAGTGGNTCANTGGGAANGGGGGAGTGTATGGCAANACG
  AGTCACTTNCCATTACACATGTNCGTCTGCTGGATCAACAGAANTCCGATGAGGGACTCA
  GCTGAGTATAGNTCAACAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005206 unedited
  TTTTTTGCTTTGTACCGGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAG
  ACTGAGTCTTGCCCTGTCGCTCAGGCTGGAACGCAGTGGTGCGATCTCGGCTCACTGCAG
  CCTCCACCTCCCAGGTTGAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTAC
  AGGCGTGCACCACCACGCCGGGCTAATTTTTTATATCTTTAGCAGAGATGGGGTCTCACT
  ATGTCGGCCAGGCTGGCCTCGAACTCCTGACCTCATGATCCACCCGCCTCGGCCTTCCAA
  AGTGCTGGGATTACAGACGTGAGCCACCGTGCCCAGTCGGTCCAACATTTTTTTAAAGCA
  CTAACCCTACCCCAAATTCCTGTCACTACTAGCCCCGCCTCTTATCCCCGTTGCACCCCC
  TCGCAGACGGCCCCAACCTGACCCCTAAACCCTCCCCCACCACTATTATTAGGTGATATG
  TTCCCTGGGACCCACCCGACCCCCACCTTCGCGACCCCCCCCGAGGCTGCCTACTCCGCC
  TCCCGCCCCCTCTCGCCCTACACGGCTCGTGTCGAGACATCCTAAATTTTCTTCCTTCTA
  ATCATCCCCCCTCCCCTCCTTTTCGCATTCCCCCCCGTCCCCCCTCTCCCGCAACTCTTA
  CACTATCTCCCCTTCCCCCCCCTCCGCCCCCCCACCCACCCCCACACCCCGCCTTCCTCC
  TCCGCCCCCACCATGGTTTTCTCCCTCTTCCGCCGCCCTCTCNTCCCACATTATATTAAT
  TAATTTCGCGGCCCCCGCCCGAACCGCCCTGCGTACCCTCTCGTTGTTGTGCACCTCCTT
  TCTCTTTCCCTTCCCTTCCTTCCCTCTACACCATNTTCCCCCCCTATCCCTCCTCATTCA
  TGATTTCCCGCCCCCGCGATAGCCCTACAGGCCACTCCCCCCGCCGCTTCGCGTCACCAC
  CGCTCCCCCGCTCGTATCTCCCTGCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005206
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005206.3, NP_005197.3
• RefSeq Size: 2245 bp
• RefSeq ORF: 615 bp
• Locus ID: 1398
• UniProt ID: P46108
• Cytogenetics: 17p13.3
• Domains: SH2, SH3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Regulation of actin cytoskeleton, Renal cell carcinoma
• Gene Summary: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (I) represents the shorter transcript and encodes the shorter isoform (b). Isoform b increases transformation activity due to the loss of a SH3 domain.