CDC42 (NM_044472) Human Untagged Clone

CAT#: SC109056

CDC42 (untagged)-Human cell division cycle 42 (GTP binding protein, 25kDa) (CDC42), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC42
• Synonyms: CDC42Hs; G25K; TKS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_044472 edited
  GAATTCGGCACGAGGCTGAGGTCATCATCAGATTTGAAATATTTAAAGTGGATACAAAAC
  TATTTCAGCAATGCAGACAATTAAGTGTGTTGTTGTGGGCGATGGTGCTGTTGGTAAAAC
  ATGTCTCCTGATATCCTACACAACAAACAAATTTCCATCGGAATATGTACCGACTGTTTT
  TGACAACTATGCAGTCACAGTTATGATTGGTGGAGAACCATATACTCTTGGACTTTTTGA
  TACTGCAGGGCAAGAGGATTATGACAGATTACGACCGCTGAGTTATCCACAAACAGATGT
  ATTTCTAGTCTGTTTTTCAGTGGTCTCTCCATCTTCATTTGAAAACGTGAAAGAAAAGTG
  GGTGCCTGAGATAACTCACCACTGTCCAAAGACTCCTTTCTTGCTTGTTGGGACTCAAAT
  TGATCTCAGAGATGACCCCTCTACTATTGAGAAACTTGCCAAGAACAAACAGAAGCCTAT
  CACTCCAGAGACTGCTGAAAAGCTGGCCCGTGACCTGAAGGCTGTCAAGTATGTGGAGTG
  TTCTGCACTTACACAGAGAGGTCTGAAGAATGTGTTTGATGAGGCTATCCTAGCTGCCCT
  CGAGCCTCCGGAAACTCAACCCAAAAGGAAGTGCTGTATATTCTAAACTGTTTTCTCCTT
  CCCTTCTTTGCTGCTGCTTCCTGTCCCACTACTGTAGAAAGATCGTTTAAAAACAAAGGA
  ATAAAACCATCCTGTTTGAAAGCCTCTGCGTCTTTTTACTCACCACCTTAXXXXXXXXXX
  XXXXCTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_044472 unedited
  AGTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTGAGGTCAT
  CATCAGATTTGAAATATTTAAAGTGGATACAAAACTATTTCAGCAATGCAGACAATTAAG
  TGTGTTGTTGTGGGCGATGGTGCTGTTGGTAAAACATGTCTCCTGATATCCTACACAACA
  AACAAATTTCCATCGGAATATGTACCGACTGTTTTTGACAACTATGCAGTCACAGTTATG
  ATTGGTGGAGAACCATATACTCTTGGACTTTTTGATACTGCAGGGCAAGAGGATTATGAC
  AGATTACGACCGCTGAGTTATCCACAAACAGATGTATTTCTAGTCTGTTTTTCAGTGGTC
  TCTCCATCTTCATTTGAAAACGTGAAAGANAAGTGGGTGCCTGAGATAACTCACCACTGT
  CCAAAGACTCCTTTCTTGCTTGTTGGGACTCAAATTGATCTCAGAGATGACCCCTCTACT
  ATTGAGAAACTTGCCAAGAACAAACAGAAGCCTATCACTCCAGAGACTGCTGAAAAGCTG
  GCCCGTGACCTGAAGGCTGTCAAGTATGTGGAGTGTTCTGCACTTACACAGAGAGGTCTG
  AAGAATGTGTTTGATGAGGCTATCCTAGCTGCCCTCGAGCCCTCGGAACTCNACCCAAAA
  GAAGTGCTGTATATTCTAAACTGGTTTCTCCTTCCCTTNCTTGCTGCTGCTTCCTGTCCC
  ACTACTGTAGAAAGATCGTTTAANAACAAAGGAATAAAACATCCTGTTTGAAAGCCTCTG
  CGTCCTTTTACTCACCACCTAGAGCACCTCTGTATTAGTTTTTGATCAAGAATGCATTAT
  CATATAAATTTTTTGGGATCAGTAGCCAAGTGGGACTTGTTTTAAACGTTCTGCTGCCTG
  GATTGGCCTGAAGCTCAAAGCTTTTTGGTTGGAATACTATTGGCAAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_044472 unedited
  GGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTAACAAACGGGTGTATATTTTTAATTTCAAAATTTGAAGGTAACCACAATTGTTT
  TTTTCCCTCACAATAAAATCAAAAGCCGGAAAATATAAAGGGGGCAGCTTTACATCTCAA
  CATTAACAAACTGTAAGTCAACTGTTTAAATGGATGTTATTCTTAAAAAATAATGTAACA
  AATGAACTCATAAAGCAGTAGCAAATACCTCAATTTCTTCCCCACCCCAAACTGACAAAT
  ACTCCACTACAGCACAAACAGCCAAAGCAACAGCAACACAAAGTTTCACCGCCCAAAGGA
  AAACCAGAAAAGAATCCTCCCTTCCCCATTACACTTTACTAGGGCACCCCTTTTCCATAA
  GGGTTGGCATGCAATTACAATTAACAATTGATCATAAAAGTAAAAGGATGTTTGGGTTAC
  TTTCTCCCTCTAAAACTAAAATCTAAAAGTGTTAAAACTCTTGTTATTTTCTCCAAAAGG
  ACATTCTTAAAGCCAAACCAAGTTCCCTTTTGCAATAGTAATCCAAACCAAAAAGCTCTG
  AGCATCAGGCAACTCAAGCAGCAGAACGTTAAAACAAGTCCAACTTGACTCCTGATCACA
  AAAAATTTATTTGATATTGCCTTCTGGATCAAAACTATACAGAGGTTGCTCTAAGGGGGG
  AGTAAAAAACACGAAGCTTTAAACAGGAGGTTTTATTCCTTGTTTTAAAAATCTTTTTCA
  GTTGTGGGCANGAACCCCNCCAAAAAAAGGAAGAAAAAACAGTTAAAATTTCCCCCCTTC
  CTTTAGGTGTGATTTCCCGAGGCCCAGGCAACTAGATAACCTATAAAACACTTTTAAACC
  TCTGGGAAGGGAAAACCCCCATCTTGAAGCCTTAGGCACGGGCACTTTTAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_044472
• Insert Size: 1460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_044472.1, NP_426359.1
• RefSeq Size: 1135 bp
• RefSeq ORF: 576 bp
• Locus ID: 998
• UniProt ID: P60953
• Cytogenetics: 1p36.12
• Domains: ras, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Axon guidance, Chemokine signaling pathway, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Fc gamma R-mediated phagocytosis, Focal adhesion, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, Pancreatic cancer, Pathogenic Escherichia coli infection, Pathways in cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Tight junction, VEGF signaling pathway
• Gene Summary: The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
  Transcript Variant: This variant (2) has a novel 3' structure, resulting in a distinct 3' coding region compared to variant 1. The encoded isoform (2) is the same length as isoform 1 but has a distinct protein sequence. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.